Open tubular capillary electrochromatography: A useful microreactor for collagen I glycation and interaction studies with low-density lipoprotein particles

Diabetes, a multifunctional disease and a major cause of morbidity and mortality in the industrialized countries, strongly associates with the development and progression of atherosclerosis. One of the consequences of high level of glucose in the blood circulation is glycation of long-lived proteins, such as collagen I, the most abundant component of the extracellular matrix (ECM) in the arterial wall. Glycation is a long-lasting process that involves the reaction between a carbonyl group of the sugar and an amino group of the protein, usually a lysine residue. This reaction generates an Amadori product that may evolve in advanced glycation end products (AGEs). AGEs, as reactive molecules, can provoke cross-linking of collagen I fibrils. Since binding of low-density lipoproteins (LDLs) to the ECM of the inner layer of the arterial wall, the intima, has been implicated to be involved in the onset of the development of an atherosclerotic plaque, collagen modifications, which can affect the affinity of native and oxidized LDL for collagen I, can promote the entrapment of LDLs in the intima and accelerate the progression of atherosclerosis.In this study, open tubular capillary electrochromatography is proposed as a new microreactor to study in situ glycation of collagen I. The kinetics of glycation was first investigated in a fused silica collagen I-coated capillary. Dimethyl sulphoxide, injected as an electroosmotic flow marker, gave information about the charge of coating. Native and oxidized LDL, and selected peptide fragments from apolipoprotein B-100, the protein covering LDL particles, were injected as marker compounds to clarify the interactions between LDLs and the glycated collagen I coating. The method proposed is simple and inexpensive, since only small amounts of collagen and LDL are required. Atomic force microscopy images complemented our studies, highlighting the difference between unmodified and glycated collagen I surfaces.     

A new sample preparation method for determination of acidic drugs in sewage sludge applying microwave assisted solvent extraction followed by gas chromatography-mass spectrometry

This paper presents a new sample preparation procedure for determination of selected acidic pharmaceuticals (ibuprofen, naproxen, ketoprofen, and diclofenac) in sewage sludge using microwave assisted solvent extraction, dispersive matrix extraction (DME) followed by the conventionally applied solid phase extraction (SPE), derivatization, and gas chromatography-mass spectrometry. The recoveries calculated from analytical data of spiked sludge samples changed in the range of 80-105% ± 15% for the four pharmaceuticals in mixed and activated sludge depending on the efficiency of the clean-up procedure. The measured concentration values of ibuprofen and naproxen were identical in the mixed and the activated sludge samples. However, ketoprofen and diclofenac showed about twice as high concentration in activated sludge than in the mixed one independently of the applied extraction method. The typical concentration ranges of ibuprofen, naproxen, ketoprofen and diclofenac in sewage sludge were 10-30 ng/g, 30-50 ng/g, 50-130 ng/g, and 50-140 ng/g respectively.     

Water Adsorption on Platinum Dioxide and Dioxygen Complex: Matrix Isolation Infrared Spectroscopic and Theoretical Study of Three PtO2–H2O Complexes

The interactions of water molecule with platinum dioxygen complex and dioxide molecule are investigated by means of matrix isolation infrared spectroscopy and density functional calculations. The platinum atoms reacted with dioxygen to form the previously reported Pt(O₂) complex. The Pt(O₂) complex reacted with water molecule to give the Pt(O₂)–H₂O complex, which was characterized to involve hydrogen bonding between one O atom of Pt(O₂) and one H atom of H₂O (structure A ). Upon visible light irradiation, the hydrogen bonded Pt(O₂)???HOH complex rearranged to another Pt(O₂)–H₂O isomer (structure B ), which involves (O₂)Pt???OH₂ interaction. The Pt(O₂)–H₂O complex in structure B can be isomerized to the weakly bound platinum dioxide‐water complex (structure C ) under UV irradiation.     

The Design of Polymer‐based Nanocarriers for Effective Transdermal Delivery

This study reports a facile and practical means to non‐invasively deliver biologically active ingredients through the skin using polymer‐based nanocarriers. For this, polymer nanocapsules were fabricated with different surface charges as well as glass transition temperatures and we observed their ability to deliver the encapsulated active ingredient, coenzyme Q10, through the skin layer. Direct imaging of a probe molecule, Nile Red, and a matrix polymer labeled with fluorescence moiety, Lucifer Yellow, allowed us to demonstrate that the probe molecule readily permeates into the deep skin, while the matrix polymer stays in the stratum corneum layer due to electrostatic interactions. Quantitative characterization of the penetrating amount of coenzyme Q10 using the Frantz cell method proved that, to achieve improved delivery efficiency, the nanocapsule should have a low glass transition temperature as well as positive surface charges.

     

A convenient purification and preconcentration of peptides with α‐cyano‐4‐hydroxycinnamic acid matrix crystals in a pipette tip for matrix‐assisted laser desorption/ionization mass spectrometry

Peptide samples derived from enzymatic in‐gel digestion of proteins resolved by gel electrophoresis often contain high amount of salts originating from reaction and separation buffers. Different methods are used for desalting prior to matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry (MS), e.g. reversed‐phase pipette tip purification, on‐target washing, adding co‐matrices, etc. As a suitable matrix for MALDI MS of peptides, α‐cyano‐4‐hydroxycinnamic acid (CHCA) is frequently used. Crystalline CHCA shows the ability to bind peptides on its surface and because it is almost insoluble in acidic water solutions, the on‐target washing of peptide samples can significantly improve MALDI MS signals. Although the common on‐target washing represents a simple, cheap and fast procedure, only a small portion of the available peptide solution is efficiently used for the subsequent MS analysis. The present approach is a combination of the on‐target washing principle carried out in a narrow‐end pipette tip (e.g. GELoader tip) and preconcentration of peptides from acidified solution by passing it through small CHCA crystals captured inside the tip on a glass microfiber frit. The results of MALDI MS analysis using CHCA‐tip peptide preconcentration are comparable with the use of homemade POROS R2 pipette tip microcolumns. Advantages and limitations of this approach are discussed. Copyright © 2009 John Wiley & Sons, Ltd.     

含葡萄糖的混浊介质的Mueller矩阵模拟与分析

利用矢量Monte Carlo算法结合葡萄糖的旋光特性模拟了含糖混浊介质的背散射Mueller矩阵.数值模拟表明:含糖混浊介质的背散射Mueller矩阵元素中除Mll、M14、M41、M44外的其它元素花样均发生龙卷风状的不同程度的扭曲,其中M24、M34、M42、M43的扭曲程度更为显著.研究发现M24、M34、M4...     

特征值问题的预变换方法(I): 杨辉三角阵变换与二阶PDE 特征多项式

本文提出一类求解特征值问题的下三角预变换方法, 目标是通过相似变换后矩阵下三角元素平方和明显减少、且变换后的特征值及其特征向量较易求解, 使变换后的对角线可作为全体特征值很好的一组初值, 其作用如同对于解方程组找到好的预条件子, 加速迭代收敛. 以二阶PDE 数值计算为例,对于以Laplace 方程为代表的特征波向量组及正交多项式组有广泛的应用前景.
杨辉三角是我国古代数学家的一项重要成就. 本文引入杨辉三角矩阵作为预变换子, 给出一般矩阵用杨辉三角矩阵作为左、右预变换子时变为上三角矩阵的充要条件, 给出了元素为行指标二次多项式的两个矩阵类(三对角线阵与五对角线阵) 中特征值何时保持二次多项式的充要条件, 并应用于构造新的二元PDE 正交多项式.     

交换环上的严格上三角代数上的双导子

Let Nn（R）be the algebra consisting of all strictly upper triangular n × n matrices over a commutative ring R with the identity.An R-bilinear map φ ：Nn（R）×Nn（R）→ Nn（R）is called a biderivation if it is a derivation with respect to both arguments.In this paper,we define the notions of central biderivation and extremal biderivation of Nn（R）,and prove that any biderivation of Nn（R）can be decomposed as a sum of an inner biderivation,central biderivation and extremal biderivation for n ≥ 5.     

Incomplete active polarimetry: Measurement of the block-diagonal scattering matrix

The problem of measurement of block-diagonal scattering matrix as being part of the so-called incomplete or partial Mueller polarimetry is addressed. It has been shown that non-zero elements of the scattering matrix with block-diagonal structure can be measured using only two elliptical polarizations of input light. Optimal pairs of these polarizations are derived. Utilization of the optimal input polarizations results in halving measurement time and decreasing measurement errors by 30%.     

A novel terahertz wave reflective polarizer for THz communication

We design terahertz wave reflective polarizer that operates over a wide terahertz wavelength range and is based on a periodic bilayer structure. The structure is characterized by transfer matrix calculations. Results of simulations show that the mirror is highly reflecting for incidence angle θ ≤ 60°and TE as well as TM polarization in the wavelength range between 541.6 μm and 574.2 μm (i.e. frequency band between 522.5GHZ and 533.9 GHZ). As the incidence angle increases this reflection band blueshifts for both TM and TE polarizations.