Laser Microbeams and Optical Tweezers in Ageing Research

We show how a technique developed within the framework of physics and physical chemistry—in a true interdisciplinary approach—can answer questions in life sciences that are not solvable by using other techniques. Herein, we focus on blood‐pressure regulation and DNA repair in ageing studies. Laser microbeams and optical tweezers are now established tools in many fields of science, particularly in the life sciences. A short glimpse is given on the wide field of non‐age‐research applications in life sciences. Then, optical tweezers are used to show that exerting a vertical pressure on cells representing the inner lining of blood vessels results in bursts of NO liberation concomitant with large changes in cell morphology. Repeated treatment of such human umbilical vein endothelial cells (HUVEC) results in stiffening, a hallmark of manifest high blood pressure, a disease primarily of the elderly. As a second application in ageing research, a laser microbeam is used to induce, with high spatial and temporal resolution, DNA damages in the nuclei of U2OS human osteosarcoma cells. A pairwise study of the recruitment kinetics of different DNA repair proteins reveals that DNA repair starts with non‐homologous end joining (NHEJ), a repair pathway, and may only after several minutes switch to the error‐free homologous recombination repair (HRR) pathway. Since DNA damages—when incorrectly repaired—accumulate with time, laser microbeams are becoming well‐used tools in ageing research.     

生长条件对KDP 晶体激光损伤阈值的影响

 研究了各种生长条件对晶体激光损伤阈值的影响, 发现提纯原料用的EDTA对晶体的光损伤阈值没有显著的影响, 溶液陈化能够降低晶体的散射强度,从而使光损伤阈值提高。多磷酸盐阻碍晶体锥面生长, 影响晶体的生长习性, 并降低晶体的激光损伤阈值。     

连续CO2激光对红外窗口材料损伤研究

研究了连续ＣＯ２激光对几种红外窗口材料的表面损伤特性，研究表明，损伤机制在于杂质缺陷吸收造成的热冲击应力破坏。深入研究了杂质缺陷密度、焦斑与材料损伤阈值的关系，并建立了一个损伤模型。同时分析了热冲击应力破坏方式。     

Surface instability of orthotropic laminated materials under compression

The piecewise-homogeneous material model and the three-dimensional linearized theory of stability with the assumption of small subcritical strains are used to study the surface buckling of orthotropic and transtropic laminates. A plane problem is formulated, and characteristic equations are derived. A solution is found for a specific transtropic material with different orientations of the isotropy axis __________ Translated from Prikladnaya Mekhanika, Vol. 42, No. 7, pp. 64–72, July 2006.     

Micro-Raman analysis on LiNbO3 substrates and surfaces: Compositional homogeneity and effects of etching and polishing processes on structural properties

In this work we report on micro-Raman analysis on lithium niobate (LN) substrates in order to study the compositional homogeneity of the crystals and to clear up the effects of etching and polishing processes on the surface of wafers and crystals.The fact that the linewidth of some Raman modes scale with the composition of LN crystals, together with the use of a confocal microscope, allowed a three-dimensional determination of the sample stoichiometry and of the crystalline quality. This local tool can supply additional information, which can be complementary to the electro-optic coefficients, carefully measured as well in order to check functional parameters.Raman spectra from buried regions were obtained on as-grown, etched and polished crystals and wafers. The depth profile of the peak energy and the linewidth of the Raman mode at 872 cm⁻¹ indicate that mechanical processing of surfaces causes, in some cases, structural modifications till a depth of 15 μm.     

A stochastic theory of cumulative fatigue damage

A stochastic theory for the cumulative fatigue damage of structural components with random fatigue strength under random loading is proposed on the basis of Stratonovich-Khasminskii theorem. The analytical solutions for the probability densities of the cumulative fatigue damage and fatigue life and for the reliability function are given for steel and reinforced concrete components with constant fatigue strength subject to a narrow band stationary Gaussian stress process with zero mean. The results agree very well with those of digital simulation. It is noted that the theory can be applied, in principle, to both metallic and non-metallic materials, narrow band and wide band stress process, and adapted to a sequence ofn, stationary stress processes or quasi-stationary stress process. The scatter and degradation of fatigue strength and the inspection maintenance can also be incorporated into the theory.     

飞秒激光烧蚀石英玻璃的实验与理论研究

实验研究了800nm飞秒激光作用下石英玻璃的破坏机理和烧蚀规律，给出了破坏阈值与脉冲宽度的关系.发展了雪崩击穿模型，计算了材料的烧蚀阈值与脉冲宽度的依赖关系，烧蚀深度、烧蚀体积与脉冲能量的依赖关系，研究了导带电子的扩散对材料中激光能量的沉积、分布，以及材料的破坏阈值和烧蚀规律的影响. 关键词： 飞秒激光脉冲 破坏机理 石英玻璃 电子扩散     

Calculation of the Thermoelastoplastic Nonaxisymmetric Stress-Strain State of Layered Orthotropic Shells of Revolution

The methods for determining the nonaxisymmetric thermoelastoplastic stress-strain state of layered orthotropic shells of revolution are developed. It is assumed that the layered package deforms without mutual slippage or separation of layers. The problem is solved using the geometrically nonlinear theory of shells based on the Kirchhoff-Love hypotheses. In the isotropic layers, plastic deformations may appear, whereas the orthotropic layers deform in the elastic region. It is assumed that the mechanical properties of the materials are temperature-dependent. The thermoplasticity equations are presented in a form corresponding to the method of additional deformations. The order of the system of partial differential equations obtained is reduced with the help of trigonometric series in the circumferential coordinate. The resulting systems of ordinary differential equations are solved by the Godunov technique of discrete orthogonalization. The nonaxisymmetric thermoelastoplastic stress-strain states of layered shells of revolution are considered as examples.     

韧性断裂的微观模型及其在约束断裂中的应用

详细分析了不同形状断裂试件及小范围屈服模型裂纹端部的损伤演化，提出了韧性断裂的宏观起裂相当于裂尖前方一特征位置处的损伤达到一临界值。利用此模型获得了与实验相一致的宏观断裂韧性及与约束无关的理论断裂韧性。     

硅酸铋(BSO)晶体闪烁性能研究

报道对BSO晶体闪烁性能研究的若干结果. 包括晶体的激发光谱和荧光光谱、光产额、发光衰减时间和抗辐照能力等特性.