稳定表达egfp基因细胞的构建与克隆

将增强的绿色荧光蛋白（enhanced green fluorescent pprotein,EGFP）基因插在HCMV（humancytomegolovirus）启动子下游,构建了表达质粒pCA13-eG,用脂质体Lipofectin介导分别转染HeLa细胞、Vero细胞,仅通过细胞传代,就最能稳定高效表达EGFP的绿色细胞,比较发现,质粒pCS13-eG转染后,产生能高效表达EGFP的HeLa细胞其比率高于Vero细胞;EGFP高效表达对Vero细胞的毒性大于对HeLa细胞的毒性,本研究表明,绿色细胞轮廓清晰,由于其特有的性质,在用于细胞的形态观察、细胞分裂等研究时会有所作为。     

超导探测器与太赫兹天文应用

太赫兹频段(0.1—10 THz)占有宇宙空间近一半的光子能量,特别适合观测早期遥远天体、正在形成冷暗天体,以及被尘埃遮掩天体,且具有非常丰富的分子、原子及离子谱线,是其他频段不可替代的宇宙观测窗口。近四十年来,低温超导探测器技术得到快速发展,在天文学领域率先实现应用并取得系列有显著影响的研究成果,如黑洞成像、原行星盘精细结构观测以及近邻宇宙水分子刻画和宇宙最先诞生的电离氢化氦离子探测等。文章将主要介绍4种国际主流太赫兹超导探测器(即超导隧道结(SIS)混频器、超导热电子(HEB)混频器、超导相变边缘(TES)探测器和超导动态电感(KID)探测器)的研究进展、应用突破和未来发展趋势。     

Satellite infrared imagery, rawinsonde data, and gravity wave remote sensing of severe convective storms

GOES digital infrared data during the time period between two hours before the touchdown of tornado and the tornado touchdown time were used in this study. Comparison between tornado-associated clouds and non-tornado-associated clouds indicates that the difference between overshooting cloud top temperature and the tropopause temperature, or how much the cloud has penetrated above the tropopause, rather than either the absolute temperature of penetrative cloud top or the height of the top of overshooting turret is significant for the possible formation of severe storms. The penetrative overshooting cloud top collapses about 15 to 30 minutes before the touchdown of tornado. Gravity waves were detected from the severe convective storms.     

Observation,Measurement, and Computation in Finite Games

Limitations in agents ability to observe, measure and compute are studied. A formulation in terms of induced changes in the players subjective perception of the game makes it possible to study these phenomena on a common footing with objective variation in the game. We study a model where payoffs undergo continuous variation and players reason about the process from limited local information. Specifically, finite games, and statements about them, are interpreted within sheaf models, which incorporate both variation and a logic of limited observation. Nashs theorem on the existence of equilibrium is not valid unless special observational properties hold, although closely related statements are valid. The possibility of learning to play a mixed strategy Nash equilibrium from observation of past play is also examined within the framework.I thank the referee and associate editor for their comments and suggestions. I also received very helpful suggestions from Josh Epstein. All remaining errors are my own.Received: April 2000 / Revised: December 2003     

Thermotropic Liquid Crystalline Poly(Azomethine‐Ether)s Containing Dibenzylidene Derivatives in the Main Chain

A new homologous series of thermotropic liquid crystalline poly (azomethine‐ether)s based on dibenzylidene derivatives were synthesized by solution polycondensation of various diformyl‐α,ω-diphenoxyalkanes, I–VIII with 2,6‐bis(m‐aminobenzylidene)‐4‐phenylcyclohexanone X, and characterized by ¹H‐NMR, IR and elemental analyses. The inherent viscosities of the polymers were in the range 0.29–0.58 dI/g. All the poly (azomethine‐ether)s were insoluble in common organic solvents but dissolved completely in concentrated H₂SO₄ and methanesulphonic acid. The mesomorphic properties were studied as a function of the diphenoxyalkane space length. Analysis by DSC and optical polarized microscopy demonstrated that the poly (azomethine‐ether)s from nematic mesophases over wide temperature ranges.     

基于压缩感知的252Cf源驱动核材料浓度识别技术研究

针对252Cf源驱动噪声分析测量法中核材料浓度识别问题,采用压缩感知理论,在K最近邻(KNN)识别算法基础上,研究了一种基于压缩采样的K最近邻（CSKNN）分类识别方法,进而研究并分析了CSKNN方法的识别概率。实验结果表明,CSKNN分类识别方法只需少量的观测值(观测比M/N0.1),即可达到分类识别的目的;当信噪比提高时,识别概率将会以更快的速度收敛至100%,且对K值的敏感程度也会随之降低。这样,不仅提高了核军控核查的实时性,而且还有效降低了采样成本,为核材料浓度的在线判读提供了一种新的理论基础和实现方法。     

经纬仪测星评估惯导系统姿态角误差方法

针对动态条件航天测量船惯导系统姿态角精度鉴定和评估这个困扰多年的难题,提出一种利用经纬仪观测恒星对惯导姿态角误差进行解算的方法:用短时间(每颗星记录2 s)观测方位角大致均匀分布的多颗恒星数据解算惯导姿态角误差的稳态分量;用较长时间(每颗星记录200 s以上)观测特殊方位角单颗恒星的数据,观察惯导姿态角误差的动态特性,详细介绍了该方法数学模型的推导过程,从理论上分析了该方法各种数据误差源对解算精度的影响,并利用实际观测数据对惯导姿态角误差稳态分量和动态特性进行了解算和观察,结果与航天测量船惯导系统的设计指标基本吻合,表明该方法可以作为评估航天测量船惯导系统姿态角动态精度的一种有效手段.     

Estimating a mixing distribution in a multiple observation setting

Given unobservable i.i.d. r.v.'s M_i with c.d.f. F, and observable r.v.'s Y_ij with E(Y_ij|M_i) = M_i; we study a nonparametric estimate of F based on the Y_ij's by conditioning and kernel techniques. This investigation extends previous works of Blischke (1965) and James (1978).