High-Throughput Screening of Ten Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins, by Reversed-Phase Liquid Chromatography-Electrospray Ionisation Mass Spectrometry

Liquid chromatography-electrospray ionisation mass spectrometry was evaluated in the high-throughput analysis of microcystins and nodularins, cyanobacterial peptide hepatotoxins. Extracts originating from cyanobacterial strains and field material were separated on a 30 mm × 4 mm I.D. Merck Purospher STAR RP-18e column using a rapid gradient of aqueous formic acid and acetonitrile, ionised by electrospray technique and analysed on a Micromass Quattro II triple-quadrupole mass spectrometer operated in the selected ion recording (SIR) mode. The total analysis time per sample was 2.8 min corresponding to 514 samples a day. The system showed good robustness during a series of 320 repetitive injections of a field sample containing three major microcystins.     

Vehicle density and velocity estimation on highways for on-ramp metering control

A scheme for vehicle density and velocity estimation in a stretch of highway based on a modified cell transmission model [C. F. Daganzo, Transportation Research, Part B, 28B(4),269–287, 1994. Elsevier is presented. The scheme is intended for use with on-ramp metering control algorithms, providing local knowledge of densities and velocities that is helpful to improve on-ramp metering control performance. Estimation of density is obtained by nonlinear estimators, while velocity estimation is obtained by gradient algorithms. There is one density–velocity estimator for free traffic flow and other for congested traffic flow. Both estimator schemes work in parallel. The final estimation of density and velocity results from a convex combination of the predictions of the two estimators. This combination depends on occupancy or density measurements at the boundaries of the stretch and is produced by a fuzzy inference system. Stability and convergence of the density and velocity estimation scheme is proved by Lyapunov based techniques. Simulation results comparing measured and estimated traffic data are presented. They confirm good performance of the estimators. Research sponsored by grants UNAM PAPIIT IN110403 and CONACYT 47583.     

Design and operation characteristics of a high power transverse flow pulser sustained cw CO2 laser

A transverse flow, transverse discharge cw CO₂ laser in which de discharge is sustained by employing high repetition rate high voltage pulses has been developed. Pulser sustained discharge through electrodes of innovative design provided uniform excitation at electrical input power densities more than 10 W/cc. Laser output power more than 2.5 kW was obtained in a laser gas mixture consisting of 0.5 mbar of CO₂, 16 mbar of N₂ and 38.5 mbar of He. Design details and operational characteristics of this laser are presented.     

Estimates from Below for the Spectral Function and for the Remainder in Local Weyl’s Law

We obtain asymptotic lower bounds for the spectral function of the Laplacian and for the remainder in local Weyl’s law on manifolds. In the negatively curved case, thermodynamic formalism is applied to improve the estimates. Key ingredients of the proof include the wave equation parametrix, a pretrace formula and the Dirichlet box principle. Our results develop and extend the unpublished thesis of A. Karnaukh [Ka]. The first author was supported by NSERC, FQRNT, Alfred P. Sloan Foundation Fellowship and Dawson Fellowship. The second author was supported by NSERC and FQRNT. Received: April 2006 Revision: October 2006 Accepted: October 2006     

Lower bounds for the blow-up time in a temperature dependent Navier-Stokes flow

In this paper we consider two different initial-boundary value problems in temperature dependent viscous flow when the temperature equation has a nonlinear heat source term. When blow-up occurs we derive lower bounds for the blow-up time in each case.     

Spatial decay in a double diffusive convection problem in Darcy flow

This paper deals with a model of time-dependent double diffusive convection in Darcy flow. In particular it is concerned with the spatial decay of solutions when the flow is confined to a semi-infinite cylinder. Decay bounds for an energy expression are derived.     

小长径比垂直管气液两相流动特性分析

实验观察了小长径比垂直上流管内流型及特点,并对管入口处的压力波动特性和系统的压差波动特性进行了试验研究.结果表明:小长径比(L/D)垂直管内流型表现为泡状流、塞状流、乳沫状流、环状流和液束环状流;分别增加管线中的气量、液量,或者同时增加气液流量,均会造成垂直管入口处压力波动的均值和最大压力的增加;压力信号的概率密度(PDF)大部分呈双峰分布,也存在单峰和多峰分布;差压信号的概率密度符合正态分布,其功率谱密度同压力信号相比具有频率波动范围宽、幅值小的特点.     

液态泡沫结构及其稳定性

液态泡沫由大量气泡密集堆积在少量的表面活性剂溶液中形成，是具有高度自组织结构的典型的软物质．文章从泡沫物理学角度简要介绍了液态泡沫的结构特征和稳定性方面的研究．     

Chromatographic investigations of furocoumarins fromHeracleum genus fruits

Summary Furocoumarins from extracts ofHeracleum genus fruits were separated using normal and reversed phase TLC and HPLC and isolated in the milligram scale using overloaded systems of column chromatography. Binary or ternary solvents containing a polar modifier (methanol, diisopropyl ether or acetonitrile) were used as the mobile phases. Preliminary report on this work was presented at the 47th International Congress of Pharmaceutical Sciences of F.I.P. in Amsterdam. The Netherlands, 30 August–5 September 1987.