Analysis of post-translational modification and characterization of the domain structure of dynamin A from Dictyostelium discoideum

The post-translational modifications of the 96 kDa protein dynamin A from Dictyostelium discoideum were analyzed using Q-TOF mass spectrometry. The accurate molecular mass of the intact protein revealed a covalent modification causing an additional mass of 42 Da. The modification could be identified as N-terminal acetylation by tandem mass spectrometry. Extracted ion chromatograms for the a(1) and b(1) ion of the tryptic T1 peptide were used to detect the acetylated peptide within 54 nanoelectrospray ionization tandem mass spectra. Owing to the accurate molecular mass of the intact protein, additional covalent modifications could be excluded. In addition to the covalent modification, the domain structure of dynamin A was determined by applying a combination of limited proteolysis, sodium dodecylsulfate polyacrylamide gel electrophoresis, automated tandem mass spectrometry and protein database searching.     

The domain of partial attraction of a Poisson law

Groshev gave a characterization of the union of domains of partial attraction of all Poisson laws in 1941. His classical condition is expressed by the underlying distribution function and disguises the role of the mean of the attracting distribution. In the present paper we start out from results of the recent probabilistic approach and derive characterizations for any fixed >0 in terms of the underlying quantile function. The approach identifies the portion of the sample that contributes the limiting Poisson behavior of the sum, delineates the effect of extreme values, and leads to necessary and sufficient conditions all involving . It turns out that the limiting Poisson distributions arise in two qualitatively different ways depending upon whether >1 or <1. A concrete construction, illustrating all the results, also shows that in the boundary case when =1 both possibilities may occur.     

A characterization of brownian motion in a lipschitz domain by its killing distributions

Let (Y _t, Q^x) be a strong Markov process in a bounded Lipschitz domainD with continuous paths up to its lifetime , and let (X _t, P^x) be a Brownian motion inD. IfY _– exists in D andQ ^x(Y_– C)=P^x(X_– C) for all Borel subsetsC of D and allx, thenY is a time change ofX.     

Imaging Magnetic Domain Structures with Soft X-Ray Microscopy

The current achievements in magnetic transmission soft X-ray microscopy will be reviewed. The magnetic contrast is given by X-ray magnetic circular dichroism (X-MCD), i.e., the dependence of the absorption coefficient of circularly polarized X rays on the projection of the magnetization in a ferromagnetic system onto the photon propagation direction. X-MCD contrast can reach, e.g., at L_2,3 edges in transition metals, large values up to 50%. Combined with a soft X-ray microscope where Fresnel zone plates acting as optical elements provide a lateral resolution down at 25 nm, it allows for imaging magnetic microstructures. Specific features of this photon-based technique are the recording of images in varying external magnetic fields, an inherent chemical specificity, a high sensitivity to thin magnetic layers, due to the large contrast, and the possibility to distinguish between in-plane and out-of plane contributions. In this report, recent results obtained with the XM-1 microscope at the ALS (Berkeley/CA) demonstrate the broad applicability of this novel experimental technique to both fundamental and technological relevant issues in nanomagnetism. The future potential will be briefly outlined.     

脉冲泄流时土壤条件对冲击接地阻抗的影响

 为了探寻电流脉冲泄流时，设备的接地是否有效可靠，用FDTD法分析了脉冲泄流时均一土壤、分层土壤及局部改善土壤的电导率对冲击接地阻抗（TGR）的影响。结果表明：大地电导率对冲击接地阻抗的影响很大；对均一土壤，TGR随大地电导率的增大而减小，大地电导率越大，TGR对接地体的埋深越不敏感。对分层土壤，接地体要埋在大地电导率大的土壤层中，且不要靠近交界面；局部改善大地导电性能对降低TGR效果明显，TGR对改善区域的体积非常敏感，而当电导率大于一定值时，大地电导率的再增加，对TGR的影响不明显。     

含一般模糊弹性约束的广义模糊变量线性规划

建立并讨论了一类含有一般模糊弹性约束的广义模糊变量线性规划问题.首先,简单介绍了结构元方法并对结构元加权排序中权函数表征决策者风险态度进行了深入分析.然后选取风险中性的决策者来定义序关系,应用Verdegay模糊线性规划方法将含一般模糊弹性约束的广义模糊变量线性规划转化经典的线性规划问题,简化了原问题的求解.最后通过数值算例进一步说明了该方法的有效性.     

Cramer’s rule over residue class rings of Bézout domains

Cramer’s rule over residue class rings of Bézout domains is given.     

A new formulation of regularized meshless method applied to interior and exterior anisotropic potential problems

This paper proposes a new formulation of regularized meshless method (RMM), which differs from the traditional RMM in that the traditional formulation generates the diagonal elements of influence matrix via null-field integral equations, while our new one directly employs the boundary integral equations at the domain point to evaluate the diagonal elements. We test the present RMM formulation to two-dimensional anisotropic potential problems in finite and infinite domains in comparison with the traditional RMM. Numerical results show that the present RMM sharply outperforms the traditional RMM in the solution of interior problems, while the latter is clearly superior for exterior problems. A rigorous theoretical analysis of circular domain case also corroborates such numerical experiment observations and is provided in the appendix of this paper.     

Design and analysis of a Schwarz coupling method for a dimensionally heterogeneous problem

In the present work, we propose and analyse an efficient iterative coupling method for a dimensionally heterogeneous problem. We consider the case of a 2D Laplace equation with non‐symmetric boundary conditions coupled with a corresponding 1D Laplace equation. We first show how to obtain the 1D model from the 2D one by integration along one direction, by analogy with the link between shallow water equations and the Navier–Stokes system. Then we focus on the design of a Schwarz‐like iterative coupling method. We discuss the choice of boundary conditions at coupling interfaces. We prove the convergence of such algorithms and give some theoretical results related to the choice of the location of the coupling interface, and to the control of the difference between a global 2D reference solution and the 2D coupled solution. These theoretical results are illustrated numerically. Copyright © 2014 John Wiley & Sons, Ltd.