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981.
Müller R Marchetti M Kratzmeier M Elgass H Kuschel M Zenker A Allmaier G 《Analytical and bioanalytical chemistry》2007,389(6):1859-1868
Three different analytical techniques (planar SDS-PAGE, CGE-on-a-chip and MALDI-TOF-MS) applied for determination of the molecular
weight of intact and partly and completely de-N-glycosylated human serum glycoproteins (antithrombin III and coagulation factor IX) have been compared. N-Glycans were removed from the protein backbone of both complex glycoproteins using PNGase F, which cleaves all types of asparagine-attached
N-glycan provided the oligosaccharide has at least the length of a chitobiose core unit. Two of the applied techniques were
based on gel electrophoretic separation in the liquid phase while the third technique was the gas-phase technique mass spectrometry.
It was demonstrated that the enzymatic de-N-glycosylation generally worked well (completely or partially) with both glycoproteins (one containing only N-glycans and the second N- and O-glycans). All three methods were suitable for monitoring the de-N-glycosylation progress. While the molecular weights determined with MALDI-TOF-MS were most accurate, both gel electrophoretic
methods provided molecular weights that were too high because of the attached glycan structures.
Figure CGE-on-a-chip, SDS-PAGE and MALDI mass spectrometric pattern obtained from therapeutic glycoprotein 相似文献
982.
Colnaghi Simionato AV da Silva DS Lambais MR Carrilho E 《Journal of mass spectrometry : JMS》2007,42(4):490-496
Xylella fastidiosa (X.f.) is a plant pathogen with high levels of genomic similarity to Xanthomonas campestris pv. campestris (X.c.c.). It has been shown that X. fastidiosa synthesizes a putative diffusible signal factor (X.f.-DSF) that activates regulation of pathogenicity factor (rpf) genes in a X.c.c. reporter system, which might be involved in the regulation of pathogenesis associated genes as in X.c.c., as well as in quorum-sensing. The nature of the X.f.-DSF is not known, whereas the X.c.c.-DSF has been identified as cis-11-methyl-2-dodecenoic acid. In this work, the chemical nature of a putative X.f.-DSF molecule, able to restore endoglucanase activity in a X.c.c. rpfF mutant, was investigated as if it was a fatty acid derivative. Bioassays with X.c.c. reporter bacterium and X.f. culture extracts, based on endoglucanase restoration activity, were also carried out in order to confirm the DSFs molecules similarities. For this reason, a gas chromatography-mass spectrometry method was developed with standard fatty acids methyl esters mixtures. The retention time, as well as the fragmentation patterns, of each standard was used to identify the DSF molecule synthesized by X.f. in the culture medium. Typical ester fragmentation patterns (the derivatized analyte) were observed, such as: McLafferty rearrangement and migration of the Hdelta followed by 1,4-hydrogen shift and cleavage of the bond Cbeta-Cgamma, confirming the nature of this molecule. This confirmation was corroborated by the common peaks in both spectra. Besides, the observed retention time reinforces our conclusion since it corresponds to a methyl ester with 15 carbons. Since the X.f.-DSF molecule was tentatively identified as 12-methyl-tetradecanoic acid (by mass spectra library comparison), this standard compound was also analyzed, strongly suggesting that this is the identification of such a molecule. To our knowledge, this is the first time a DSF produced by X.f. has been characterized. 相似文献
983.
Huang LF Wu MJ Zhong KJ Sun XJ Liang YZ Dai YH Huang KL Guo FQ 《Analytica chimica acta》2007,588(2):216-223
In this paper, chromatographic fingerprint was firstly used for quality control of tobacco flavors. Based on gas chromatography-mass spectrometry (GC-MS) and combined chemometrics methods, a simple, reliable and reproducible method for developing chromatographic fingerprint of coffee flavor, one of tobacco flavors, was described. Six coffee flavor samples obtained from different locations were used to establish the fingerprint. The qualitative and quantitative analysis of coffee flavor sample from Shenzhen was completed with the help of subwindow factor analysis (SFA). Fifty-two components of 68 separated constituents in coffee flavor sample from Shenzhen, accounting for 88.42% of the total content, were identified and quantified. Then, spectral correlative chromatography (SCC) was used to extract the common peaks from other five studied coffee flavor samples. Thirty-eight components were found to exist in all six samples. Finally, the method validation of fingerprint analysis was performed based on the relative retention time and the relative peak area of common peaks, sample stability and similarity analysis. The similarities of six coffee flavor samples were more than 0.9104 and showed that samples from different locations were consistent to some extent. The developed chromatographic fingerprint was successfully used to differentiate coffee flavor from coco flavor and some little difference sample prepared with coffee flavor and coco flavor by both similarity comparison and principal component projection analysis. The developed method can be used for quality control of coffee flavor. 相似文献
984.
Compound CpMoI2(iPr2dad) (iPr2dad = iPrNCHCHNiPr), obtained by halide exchange from CpMoCl2(iPr2dad) and NaI, has been isolated and characterized by EPR spectroscopy, cyclic voltammetry, and X-ray crystallography. Its action as a catalyst in atom transfer radical polymerization (ATRP) and as a spin trap in organometallic radical polymerization (OMRP) of styrene and methyl acrylate (MA) monomers has been investigated and compared with that of the dichloro analogue. Compound CpMoCl2(iPr2dad) catalyzes the ATRP of styrene and MA with low efficiency factors f (as low as 0.37 for MA and ethyl 2-chloropropionate as initiator), while it irreversibly traps the corresponding growing radical chains under OMRP conditions. On the other hand, compound CpMoI2(iPr2dad) has a greater ATRP catalytic activity than the dichloro analogue and yields f = 1 for MA and ethyl 2-iodopropionate as initiator. Under OMRP conditions, it does not irreversibly trap the growing radical chains. This comparison serves to illustrate the general principle that low initiator efficiency factors, sometimes observed in ATRP, may result from the interplay of the ATRP and OMRP mechanisms, when the latter ones involves an irreversible radical trapping process. 相似文献
985.
袁中扬 《纯粹数学与应用数学》2007,23(2):283-288
借助快速付立叶变换(FFT),本文给出一种求n阶鳞状因子循环矩阵的逆阵、自反g-逆、群逆、Moore-Penrose逆的快速算法,该算法的计算复杂性为O(nlog2n),最后给出的两个数值算例表明了该算法的有效性. 相似文献
986.
987.
消偏光纤陀螺的理论和实验研究 总被引:7,自引:5,他引:2
本文首次在理论上导出消偏型光纤陀螺的零漂和标度因子表达式,并得出以下两个结论:1)在使用约 40 dB偏振器时,导致陀螺漂移的主要因素是强度误差而并不是振幅误差;2)由光学标度因子与各种器件参量关系表明开环解调时陀螺的线性误差较大.试验样机证明了以上结果,并找出减小漂移的方法. 相似文献
988.
989.
LINEAR AND NONLINEAR SEMICLASSICAL THEORY OF WHISPERING-GALLERY-MODE MICROSPHERE LASER 总被引:1,自引:0,他引:1 下载免费PDF全文
A complete whispering-gallery-mode microsphere laser theory is developed, which combines usual laser semiclassical theory with Lorenz-Mie theory. The linear and nonlinear problems of the microsphere lasing are dealt with in the universal mode picture. The threshold condition, average amplitude of the steady state and frequency effect are obtained. A comparison between the theory and experiments is also made. 相似文献
990.
A.N. Ivanov H. Oberhummer N.I. Troitskaya M. Faber 《The European Physical Journal A - Hadrons and Nuclei》2000,7(4):519-535
The Nambu–Jona–Lasinio model of the deuteron suggested by Nambu and Jona–Lasinio (Phys. Rev. 124 (1961) 246) is formulated
from the first principles of QCD. The deuteron appears as a neutron–proton collective excitation, i.e. a Cooper np–pair, induced
by a phenomenological local four–nucleon interaction in the nuclear phase of QCD. The model describes the deuteron coupled
to itself, nucleons and other particles through one–nucleon loop exchanges providing a minimal transfer of nucleon flavours
from initial to final nuclear states and accounting for contributions of nucleon–loop anomalies which are completely determined
by one–nucleon loop diagrams. The dominance of contributions of nucleon–loop anomalies to effective Lagrangians of low–energy
nuclear interactions is justified in the large N
C expansion, where N
C is the number of quark colours.
Received: 10 March 2000 相似文献