Detection of processed genetically modified food using CIM monolithic columns for DNA isolation

The availability of sufficient quantities of DNA of adequate quality is crucial in polymerase chain reaction (PCR)-based methods for genetically modified food detection. In this work, the suitability of anion-exchange CIM (Convective Interaction Media; BIA Separations, Ljubljana, Slovenia) monolithic columns for isolation of DNA from food was studied. Maize and its derivates corn meal and thermally pretreated corn meal were chosen as model food. Two commercially available CIM disk columns were tested: DEAE (diethylaminoethyl) and QA (quaternary amine). Preliminary separations were performed with standard solution of salmon DNA at different pH values and different NaCl concentrations in mobile phase. DEAE groups and pH 8 were chosen for further isolations of DNA from a complex matrix-food extract. The quality and quantity of isolated DNA were tested on agarose gel electrophoresis, with UV-scanning spectrophotometry, and by amplification with real-time PCR. DNA isolated in this way was of suitable quality for further PCR analyses. The described method is also applicable for DNA isolation from processed foods with decreased DNA content. Furthermore, it is more effective and less time-consuming in comparison with the existing proposed methods for isolation of DNA from plant-derived foods.     

SARS CoV内对照RNA病毒样颗粒的构建和表达

在SARS-CoV的核酸检测方法中,由于普遍缺乏安全、稳定、特异的内对照,而不能对样品处理、反转录、扩增以及定量检测实施全程监控。构建的病毒样核蛋白颗粒内对照,能够对SARS-CoV临床检测实施监控。本文通过克隆1.7Kb大肠杆菌噬菌体MS2的装配蛋白和壳蛋白基因以及SARS冠状病毒经过突变产生的270bp内对照片断,并将这些基因连接到载体pTrc99a上表达,进行纯化、定量分析、RT-PCR检测和稳定性试验。获得SARS内对照病毒样核蛋白颗粒,在人血清和SM缓冲液中37℃稳定性可达到30天,能抵抗核糖核酸酶降解。将内对照颗粒加入临床样本中一同检测,能够对检测的全过程(样品处理、反转录和PCR)有效进行监控,与SARS-CoV没有交叉反应。研究表明制备的该病毒样核蛋白颗粒稳定、安全、可靠,可作为SARS冠状病毒RT-PCR检测、定量分析的有效内对照参考品。     

亚运会兴奋剂检测

本文建立了检查IOC禁用的五大类100种药物的筛选和确证方法。对药物检查机构及资格认可,药物检验的一般步骤,第十一届亚运会兴奋剂的检测均作了简要的介绍。     

Characterisation of Sol-Gel TiO2 Films by Etching

This article describes the SmartKom Companion, one of three demonstrators developed within the SmartKom project whose goal has been to provide an intuitive and intelligent interface for non-expert users to everyday computer-based applications in different scenarios of use. Conceived for the SmartKom Mobile scenario, the Companion allows intuitive multimodal interaction for drivers and pedestrians. The development of the SmartKom prototypes was constantly accompanied by Wizard-of-Oz experiments as well as system evaluations at different stages of the project. Some usability evaluation results relative to the SmartKom Companion will also be presented.     

一种含有亚微米孔洞的新型微孔磷酸盐晶体材料的合成及其生长机理

采用水热法成功地合成了一种含有亚微米级孔洞的微孔磷酸盐晶体材料(记为HAP-TAP),其形貌特征是:六棱柱形的晶体表面分布着大量0.4～0.8μm的亚微米级孔洞,孔洞内长有片层状晶体.在样品晶化的过程中,通过控制合成时间,获得了纯六棱柱形晶体的大单晶(记为HAP).使用扫描电子显微镜(SEM)、粉末X射线衍射(XRD)、红外光谱和电子能谱(EDX)对HAP-TAP独特形貌的形成机理进行了研究和揭示.HAP的单晶XRD数据表明,HAP是一种具有二维空旷骨架结构的新型微孔磷酸铝晶体,其分子式为Al5(OH)2(PO4)7(C2N2H10)3.0.5H2O.EDX分析结果表明,生长于六棱柱形晶体孔洞内的片层状晶体为磷酸钛铝材料.     

兴奋剂检测中的化学衍生化方法

本文评述了兴奋剂检测中的各种化学衍生化方法,对蛋白同化激素、麻醉镇痛剂、β-阻断剂和利尿剂分别进行了讨论。这四类药物结构各不相同,但绝大多数属于高沸点、难挥发的极性化合物,不适于直接进行GC或GC/MS分析。通过化学衍生化.不仅可增大试样的挥发性和稳定性,减小样品的极性,使原来难以进行GC分析的试样转变成适合于色谱分析的试样,而且通过衍生化还可达到改善分离效果、提高检测灵敏度的目的。     

Existence of finitely optimal solutions for infinite-horizon optimal control problems

In this paper, we investigate the existence of finitely optimal solutions for the Lagrange problem of optimal control defined on [0, ) under weaker convexity and seminormality hypotheses than those of previous authors. The notion of finite optimality has been introduced into the literature as the weakest of a hierarchy of types of optimality that have been defined to permit the study of Lagrange problems, arising in mathematical economics, whose cost functions either diverge or are not bounded below. Our method of proof requires us to analyze the continuous dependence of finite-interval Lagrange problems with respect to a prescribed terminal condition. Once this is done, we show that a finitely optimal solution can be obtained as the limit of a sequence of solutions to a sequence of corresponding finite-horizon optimal control problems. Our results utilize the convexity and seminormality hypotheses which are now classical in the existence theory of optimal control.This research forms part of the author's doctoral dissertation written at the University of Delaware, Newark, Delaware under the supervision of Professor Thomas S. Angell.     

Sufficient conditions for a strong relative minimum in an optimal control problem

In this paper, on the basis of Young's method (Ref. 1), sufficient conditions for a strong relative minimum in an optimal control problem are given. Young's method generalizes geodesic coverings and the simplest Hilbert integral from the standard variational calculus. This paper carries Young's method over to nonparametric problems.     

司帕沙星软膏的制备与质量控制

为研制司帕沙星软膏，建立其含量测定方法。用差示紫外分光光度法在306nm外测定司帕沙星的含量。平均回收率为99．98％，RSD为0．72％，线性范围15．62－40．14μg/ml。该法简便、准确、快捷。     

微机控制离子色谱仪自动操作与数据处理系统

用转换器和ＩＢＭＰＣ／ＸＴ微机与Ｄｉｏｎｅｘ２０２０ｉ型离子色谱仪连接组成自动控制与数据处理系统，转换器由８０３１及８２５５等芯片组成，其功能是采集数据和转送数据，软件由汇编及Ｃ语言编制。这套新系统增加了注入／记录同步，分级梯度淋洗，自动连续进样等功能以及很强的数据处理能力。