Feasibility of sterilizing traditional Chinese medicines by gamma-irradiation

The feasibility of sterilizing traditional Chinese medicine (TCMs) by γ-irradiation has been systematically evaluated by the biological, toxicological and physicochemical tests on irradiated hundreds of TCMs. Those TCMs investigated in general show no significant biological or toxicological changes after irradiation, yet physicochemical changes are detectable in some irradiated TCMs, and water in TCMs enhances the effects. Those results obtained from radiolysis of some major effective components of TCMs in aqueous or ethanolic solutions reveal that the site selection of radiolytically generated radicals follows the example of simple compounds with same function groups. Wholesomeness and chemical clearance present a bright future to sterilizing TCMs by γ irradiation, however, some important measures and steps should be adopted: (1) The producers must strictly execute manufacturing procedure to reduce microbiological contamination thus lower the applied dose for sterilization which is recommended to be controlled under 5, 7 or 10 kGy, 10 kGy for dry herb, 7 kGy for herbal medicine and 5 kGy for some special herbal medicine; (2) Herb to be sterilized by γ-irradiation should exist in possible dry state; (3) Powder TCMs is recommended to mix with honey forming bolus, which can minimize the decomposition of herb.     

Degradable Molybdenum Oxide Nanosheets with Rapid Clearance and Efficient Tumor Homing Capabilities as a Therapeutic Nanoplatform

Molybdenum oxide (MoOx) nanosheets with high near‐infrared (NIR) absorbance and pH‐dependent oxidative degradation properties were synthesized, functionalized with polyethylene glycol (PEG), and then used as a degradable photothermal agent and drug carrier. The nanosheets, which are relatively stable under acidic pH, could be degraded at physiological pH. Therefore, MoOx‐PEG distributed in organs upon intravenous injection would be rapidly degraded and excreted without apparent in vivo toxicity. MoOx‐PEG shows efficient accumulation in tumors, the acidic pH of which then leads to longer tumor retention of those nanosheets. Along with the capability of acting as a photothermal agent for effective tumor ablation, MoOx‐PEG can load therapeutic molecules with high efficiencies. This concept of inorganic theranostic nanoagent should be relatively stable in tumors to allow imaging and treatment, while being readily degradable in normal organs to enable rapid excretion and avoid long‐term retention/toxicity.     

In Vivo Imaging of Glycol Chitosan‐Based Nanogel Biodistribution

The preclinical development of nanomedicines raises several challenges and requires a comprehensive characterization. Among them is the evaluation of the biodistribution following systemic administration. In previous work, the biocompatibility and in vitro targeting ability of a glycol chitosan (GC) based nanogel have been validated. In the present study, its biodistribution in the mice is assessed, using near‐infrared (NIR) fluorescence imaging as a tool to track the nanogel over time, after intravenous administration. Rapid whole body biodistribution of both Cy5.5 labeled GC nanogel and free polymer is found at early times. It remains widespreadly distributed in the body at least up to 6 h postinjection and its concentration then decreases drastically after 24 h. Nanogel blood circulation half‐life lies around 2 h with the free linear GC polymer presenting lower blood clearance rate. After 24 h, the blood NIR fluorescence intensity associated with both samples decreases to insignificant values. NIR imaging of the organs shows that the nanogel had a body clearance time of ≈48 h, because at this time point a weak signal of NIR fluorescence is observed only in the kidneys. Hereupon it can be concluded that the engineered GC nanogel has a fairly long blood circulation time, suitable for biomedical applications, namely, drug delivery, simultaneously allowing efficient and quick body clearance.

     

Modeling and Dynamic Analysis of Telescopic Systems of Structural Members with Clearance

The dynamic behavior of multi-section constructions with clearance during extending and retracting motion of the sections is analyzed. First, an appropriate physical modeling is introduced before next, the governing boundary value problem is derived by applying Hamilton's principle. Then, a classical discretization procedure is used to generate a coupled system of nonlinear ordinary differential equations as the corresponding truncated mathematical model. Performing appropriate simulations to be verified by results of an alternative software package and partly validated by some preliminary experiments, the vibrational behavior of the suggested type of multi-section telescoping systems can be studied in detail.     

考虑径向内间隙的滚动轴承平衡转子系统的非线性动力稳定性

研究滚动轴承平衡转子系统在不同轴承内间隙量,不同转速下系统的稳定性及其分岔特性和混沌．考虑Hertz接触力、 滚动体通过振动和轴承径向内间隙等非线性因素建立数学模型,根据Floquet理论分析不同间隙量下滚动轴承转子系统的周期解稳定性, 找到了3种导致周期解失稳的方式:倍周期分岔失稳、拟周期分岔失稳和边界激变导致混沌失稳．通过对各间隙量下转子系统拓扑特性变化和失稳区域的研究,表明滚动轴承间隙量是影响转子系统动力稳定性的一个重要因素．     

TOPOLOGY AND VORTEX STRUCTURES OF A CURVING TURBINE CASCADE WITH TIP CLEARANCE (Ⅰ)- EXPERIMENTAL MODEL AND TOPOLOGICAL FLOW PATTERNS ON BOTH ENDWALLS AND BLADE SURFACES

ＩｎｔｒｏｄｕｃｔｉｏｎＡｓｅｘｐｅｒｉｍｅｎｔａｌｒｅｓｅａｒｃｈｓｈｏｗｓ[1]:Ｍｅａｎｗｈｉｌｅｂｌａｄｅｐｏｓｉｔｉｖｅｃｕｒｖｉｎｇｒｅｄｕｃｅｓｔｈｅｃｒｏｓｓｗｉｓｅｐｒｅｓｓｕｒｅｇｒａｄｉｅｎｔｉｎｔｉｐｃｌｅａｒａｎｃｅ,ｒｅｓｕｌｔｉｎｇｉｎｔｈｅｄｅｃｒｅａｓｅｏｆｔｈｅｒｅｌａｔｉｖｅｌｅａｋａｇｅｔｈｒｏｕｇｈｔｉｐｃｌｅａｒａｎｃｅ ,ｔｈｅｍｉｘｉｎｇｌｏｓｓｇｅｎｅｒａｔｅｄｆｒｏｍｔｈｅｉｎｔｅｒａｃｔｉｏｎｏｆｔｈｅｔｉｐｌｅａｋａｇｅａｎｄｔｈｅｃｒｏｓｓｗｉ…     

高速机构动力学研究进展

高速机械动力学及其控制是机构学研究领域的一个前沿性学科,与其内容相关者涉及多个新兴学科领域.本文首先概述了高速机构动力学基础------柔性多体动力学建模理论及周期时变机械系统动力行为研究的现状及其进展状况.其次,对机械工程中常见的典型高速机构,特别是对高速连杆、凸轮机构动力学的研究现状进行了分门别类的介绍.再次,对带间隙机构动力学的研究给予了一定的关注.相应地,对柔性机构振动主动控制的研究状况也进行了简要介绍.最后,指出上述研究领域内一些值得研究的问题.      

叶片转动对涡轮间隙内部流动影响的数值研究

机匣与叶片的相对转动是影响涡轮叶顶间隙流动的重要冈素之一.对LISA 1.5级轴流涡轮间隙内部流动的数值计算结果表明:叶片转动对涡轮间隙流动有阻塞作用.叶片静止时,由于阻塞作用消失,导致间隙入口速度增大,间隙流鼍增加,并且通过间隙的流体全部卷起形成间隙涡.同时在叶片顶部吸力面侧前缘、中部各形成一个间隙涡,使得间隙流动损失增加.而且转速下降会加剧动叶出口截面气流过偏/偏转不足现象.同时叶片静止时,间隙前部各个弦长截面内静压自间隙入口开始一直呈增加趋势,直到叶片尾缘附近截面,间隙截面内静压才逐渐稳定.     

Viral clearance using monoliths

Clearance of biological impurities is an essential part of the manufacture of biotechnology-derived products such as monoclonal antibodies (mAbs). Salt is required during manufacture to solubilize the mAb product and stabilize it against aggregation, but salt can be a problem later during impurity clearance operations. In this work, the use of a traditional quaternary amine (Q) monolith, and a new salt-tolerant monolith were evaluated for the clearance of pathogenic impurities including viruses, DNA, and host-cell protein (HCP). The impact of flow rate, salt concentration, and presence of mixtures of impurities in the feed stream were evaluated. Both monoliths cleared DNA to the limit of detection at all salt concentrations, and both cleared virus and HCP equally well at no salt. At intermediate salt, clearance of HCP was greater for the salt-tolerant monolith, and only the salt-tolerant monolith cleared virus at elevated salt. In conclusion, monoliths successfully trapped impurities such as DNA, host-cell protein, and viruses, and at flow rates far greater than traditional chromatography columns packed with beads.     

In vitro macrophage uptake and in vivo biodistribution of long-circulation nanoparticles with poly(ethylene-glycol)-modified PLA (BAB type) triblock copolymer

The effect of the PEG-grafted degree in the range of 0–30% on the in vitro macrophage uptake and in vivo biodistribution of poly(ethylene glycol)–poly(lactic acid)–poly(ethylene glycol) (PELE) nanoparticles (NPs) were investigated in this paper. The prepared NPs were characterized in terms of size, zeta potential, hydrophilicity, poly(vinyl alcohol) (PVA) residual on nanoparticles surfaces as well as drug loading. The macrophage uptake and biodistribution including plasma clearance kinetics following intravenous administration in mice of the NPs labeled by 6-coumarin were evaluated. The results showed that, except for the particles size, the hydrophilicity, superficial charges and in vitro phagocytosis amount of NPs are dependent on the PEG content in the copolymers greatly. The higher of the PEG content, the more hydrophilicity and the nearer to neutral surface charge was observed. And the prolonged circulation half-life (t_1/2) of the PELE NPs in plasma was also strongly depended on the PEG content with the similar trend. In particular for PELE30 (containing 30% of PEG content) NPs, with the lowest phagocytosis uptake accompanied the highest hydrophilicity and approximately neutral charge, it had the longest half-life in vivo with almost 12-fold longer and accumulation in the reticuloendothelial system organs close to 1/2-fold lower than those of reference PLA. These results demonstrated that the PELE30 NPs with neutral charge and suitable size has a promising potential as a long-circulating oxygen carrier system with desirable biocompatibility and biofunctionality.