2-(5-溴-2吡啶偶氮)-5-二乙氨基酚显现人体皮肤表面镀锌工具遗留印迹

研究2-(5-溴-2吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)试剂显现镀锌工具遗留印迹的方法。 考察接触时间、时间间隔、试剂浓度、是否洗手等因素对印迹强度及分辨率的影响,并对该方法的可靠性进行验证。 实验结果表明,试剂浓度1 g/L、接触时间10 s以上,间隔时间不超过4 h条件下,均能显出较好的印迹。 该方法可以灵敏地显现镀锌工具遗留印迹,对非铁金属工具遗留印迹检测进行了很好地补充。     

基于波导相位调制器的光谱色散平滑技术实验研究

利用波导相位调制器产生的正弦调频脉冲进行了光谱色散平滑技术实验研究.结果表明该铌酸锂波导相位调制器可以容易地将DFB振荡器产生的单纵模信号展宽到0.1—1.5 nm,远场分析表明焦斑扫动规律与理论计算一致,近场分析表明SSD对近场百微米尺度的调制有显著改善.本研究为高功率激光装置束匀滑方案的优化提供了参考. 关键词： 远场 近场 光谱色散平滑 惯性约束聚变     

激光波前相位因子对飞秒脉冲激光成丝动力学的影响

利用一个全阶色散非线性传输模型采用数值模拟的方法研究了初值条件中描述透镜聚焦效应的相位因子对飞秒脉冲激光成丝动力学的影响.研究发现,利用单色指数相位因子e^{－ik(0)r2/2f}来描述飞秒脉冲的透镜聚焦效应是不准确的,特别是对于周期量级超短激光脉冲的成丝现象,甚至会导致完全不同的成丝动力学过程.为了准确描述飞秒脉冲的透镜聚焦效应,必须采用准确的相位因子e^{－ik(0)r2关键词： 相位因子 成丝 飞秒脉冲 透镜聚焦}     

Probing spin-orbit quenching in Cl (2P) + H2 via crossed molecular beam scattering

In our previous work we investigated electronically non-adiabatic effects in using crossed molecular beam scattering coupled with velocity mapped ion imaging. The prior experiments placed limits on the cross-section for electronically non-adiabatic spin-orbit excitation and electronically non-adiabatic spin-orbit quenching . In the present work, we investigate electronically non-adiabatic spin-orbit quenching for which is the required first step for the reaction of Cl^* to produce ground state HCl+H products. In these experiments we collide Cl (²P) with H₂ at a series of fixed collision energies using a crossed molecular beam machine with velocity mapped ion imaging detection. Through an analysis of our ion images, we determine the fraction of electronically adiabatic scattering in Cl^* +H₂, which allows us to place limits on the cross-section for electronically non-adiabatic scattering or quenching. We determine the following quenching cross-sections σ _quench(2.1 kcal/mol) = 26 ± 21 ?², σ _quench(4.0 kcal/mol) = 21 ± 49 ?², and σ _quench(5.6 kcal/mol) = 14 ± 41 ?².     

Modeling of 31P-NMR spectra of magnetically oriented phospholipid liposomes: A new analytical solution

³¹P-NMR spectroscopy is widely used for studies of phospholipid liposomes, a commonly used model of a biological membrane. For the correct analysis of ³¹P-NMR spectra of the liposomes it is necessary to take into account that they are deformed by the magnetic field of the spectrometer. The liposomes become ellipsoidal and this affects the lineshape of the spectrum. In the present communication we suggest a new analytical formula for modeling of ³¹P-NMR spectra of the prolate phospholipid liposomes. The formula assumes a Lorentzian broadening function and exactly ellipsoidal shape of the liposomes. Based on the formula a program called P-FIT is designed for the practical analysis of the experimental multicomponent spectra of the prolate liposomes. The versatility of the program developed in a Mathematica environment is demonstrated by simulations of a number of ³¹P-NMR spectra with different complexity.     

变分同化结合广义变分最佳分析对微波散射计资料进行海面风场反演

针对微波散射计资料的海面风场反演,本文首先利用QuikSCAT卫星散射计的近实时资料,借助荷兰皇家气象学会的SeaWinds散射计数据处理模式,在不同风速和海域的情况下,采用多解方案结合二维变分同化方法进行风向的模糊去除,达到消除解的不唯一性的目的,且对4种反演结果（低速风、中速风、高速风和近海岸风）与美国国家海洋和大气管理局卫星资料中心的近实时风场数据的对比分析,说明了该技术的有效性.在此基础上,对反演得到的中速风场利用广义变分最佳分析方法进行了调整,调整后的风场结构有了进一步的改善.此方法可为我国散射计资料反演海面风场的研究提供新的思路和方法.     

Implementation of at‐line capillary zone electrophoresis for fast and reliable determination of adenovirus concentrations in vaccine manufacturing

A CZE method was validated and implemented for fast and accurate in‐process determination of adenovirus concentrations of downstream process samples obtained during manufacturing of adenovirus vector‐based vaccines. An analytical‐quality‐by‐design approach was embraced for method development, method implementation, and method maintenance. CZE provided separation of adenovirus particles from sample matrix components, such as cell debris, residual DNA and proteins. The intermediate precision of the virus particle concentration was 6.9% RSD and the relative bias was 2.3%. In comparison, the CZE method is intended to replace a quantitative polymerase chain reaction method which requires three replicates in three analytical runs to achieve an intermediate precision of 8.1% RSD. Given that, in addition, the time from sampling till reporting results of the CZE method was less than 2 h, whereas quantitative polymerase chain reaction requires 3 days, it follows that the CZE method enables faster processing times in downstream processing.     

Quasinormal Modes of the Schwarzschild Black Hole Surrounded by the Quintessence Field in Rastall Gravity

The quasinormal modes of the Schwarzschild black hole surrounded by the quintessence in Rastall gravity are studied using the sixth-order Wentzel-Kramers-Brillouin approximative approach. The effect of the Rastall parameter on the quasinormal modes of gravitational, electromagnetic and massless scalar perturbations is explored. Compared to the case of Einstein gravity, it is found that, when η < 0, the gravitational field, electromagnetic field as well as massless scalar field damp more rapidly and have larger real frequency of oscillation in Rastall gravity, while when η > 0, the gravitational field, electromagnetic field as well as massless scalar field damp more slowly and have smaller real frequency of oscillation in Rastall gravity. It is also found that the gravitational field, electromagnetic field as well as massless scalar field damp more and more slowly and the real frequency of oscillation for the gravitational perturbation, electromagnetic perturbation as well as massless scalar perturbation becomes smaller and smaller as the Rastall parameter η increases. Compared among the quasinormal frequencies of gravitational, electromagnetic and massless scalar perturbations, I find that, for fixed η, (l, n), ∈ and N_q, the oscillation damps most slowly for the gravitational perturbation, mediate for the electromagnetic perturbation and most rapidly for the massless scalar perturbation, and the real frequency of oscillation is the smallest for the gravitational perturbation, mediate for the electromagnetic perturbation and the largest for the massless scalar perturbation in Rastall gravity.     

Isotope effects in the tyrosinase catalysed hydroxylation of l-tyrosine methyl derivatives

To investigate isotope effects in the hydroxylation of [3′,5′-²H₂]-α-methyl- and [3′,5′-²H₂]-N-methyl-l-tyrosine, they were synthesised using acid catalysed isotope exchange at high temperature. The kinetic and solvent deuterium isotope effects on V_max and V_max/K_m parameters of tyrosinase in its action on methylated derivatives of l-tyrosine were determined using the non-competitive spectrophotometric method. Lineweaver–Burk plots were used to consider the inhibition type of O-methyl-l-tyrosine, revealing that it is an uncompetitive inhibitor of tyrosinase.     

复合靶指数富集的配基系统进化技术研究进展

核酸适配体是一类具有高度特异性和亲和力的单链寡核苷酸,被誉为"人工单抗",具有广阔的应用前景。它一般是通过指数富集的配基系统进化（SELEX）技术筛选获得。目前SELEX技术多局限于单一、纯化的可溶性蛋白质靶标。然而,蛋白质的纯化过程繁琐,耗时费力,而且很多靶标（如血清中的低丰度蛋白质或细胞的膜蛋白）很难纯化获得单一纯品。复合靶SELEX技术则可以避免靶标的纯化过程,能够保持靶标的天然构象,并且可以在未明确靶标的组成及结构特性的前提下,通过高通量的盲筛获得一系列特异性核酸适配体。该文主要介绍以未纯化的各种生物样本为复合靶的SELEX技术,以期为核酸适配体的筛选提供新思路。