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951.
针对600MW火电机组低压加热器系统内部泄漏故障,提出一种基于神经网络的以最大故障分离度为目标的寻优技术。采用征兆模糊计算方法对典型故障样本进行规整化处理,建立了低加内部泄漏故障诊断的神经网络模型。结合征兆缩放优化技术和神经网络诊断模型,对不同负荷下不同程度低加内部泄漏故障进行实时仿真实验。实验表明上述方法对不同负荷下程度迥异的低加故障均可得到具有高故障分离度的正确诊断结果,可准确诊断低加内部泄漏故障,具有较好的工程实用性。 相似文献
952.
Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single‐Molecule Tracking Study
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Leonhard Möckl Prof. Dr. Thisbe K. Lindhorst Prof. Dr. Christoph Bräuchle 《Chemphyschem》2016,17(6):829-835
We present a method to artificially induce network formation of membrane glycoproteins and show the precise tuning of their interconnection on living cells. For this, membrane glycans are first metabolically labeled with azido sugars and then tagged with biotin by copper‐free click chemistry. Finally, these biotin‐tagged membrane proteins are interconnected with streptavidin (SA) to form an artificial protein network in analogy to a lectin‐induced lattice. The degree of network formation can be controlled by the concentration of SA, its valency, and the concentration of biotin on membrane proteins. This was verified by investigation of the spatiotemporal dynamics of the SA‐protein networks employing single‐molecule tracking. It was also proven that this network formation strongly influences the biologically relevant process of endocytosis as it is known from natural lattices on the cell surface. 相似文献
953.
Simultaneous Voltammetric Determination of Heavy Metals by Use of Crown Ether‐modified Electrodes and Chemometrics
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Andreu González‐Calabuig David Guerrero Núria Serrano Manel del Valle 《Electroanalysis》2016,28(4):663-670
A three‐sensor array consisting of a graphite‐epoxy composite electrode (GEC), 4‐carboxybenzo‐18‐crown‐6‐GEC and 4‐carboxybenzo‐15‐crown‐5‐GEC was employed for the simultaneous determination of Cd(II), Pb(II) and Hg(II) by differential pulse anodic stripping voltammetry (DPASV). Sensors were firstly studied for the determination of Hg(II); secondly, peak current responses confirmed that all sensors showed differentiated response for the three considered metals. A response model was developed to resolve mixtures of Cd(II), Pb(II) and Hg(II) at the µg L?1 level; Discrete Wavelet Transform was selected as preprocessing tool and artificial neural network used for the modelling of the obtained responses. 相似文献
954.
刘春月 《数学的实践与认识》2016,(18):69-75
配送企业通过结成联盟、实施共同配送可以集约资源,降低各配送成本,改善社会效益.针对联盟协作下配送企业间的利润分配问题构建收益分配的基础模型,引入风险,资源,商誉、品牌、市场地位等因素进行二次修正,用数值模拟的方法,揭示共同配送联盟利益分配机理.研究结论表明,修正后的利益分配机制,能有效地避免共同配送成员"搭便车"现象及平均主义思想,更能够体现分配的科学性.对促进配送企业积极参与共同配送、保持物流联盟的稳定具有重要意义. 相似文献
955.
Dr. Aina Rebasa-Vallverdu Dr. Stefano Valente Dr. Agostino Galanti Maria Sbacchi Francesco Vicentini Laura Morbiato Beatrice Rosetti Prof. Pierangelo Gobbo 《European journal of organic chemistry》2023,26(40):e202300529
The bottom-up fabrication of synthetic cells (protocells) from molecules and materials, is a major challenge of modern chemistry. A significant breakthrough has been the engineering of protocells capable of chemical communication using bio-derived molecules and ex situ stabilised cell machineries. These, however, suffer from short shelf-lives, high costs, and require mild aqueous conditions. In this Concept Article we analyse the chemistry at the heart of protocell communication to highlight new opportunities for synthetic chemists in protocell engineering. Specifically, we (i) categorise the main bio-derived chemical communication machineries in enzyme cascades, DNA strand displacement, and gene-mediated communication; (ii) review the chemistries of these signal transduction machineries; and (iii) introduce new types of bio-inspired, fully synthetic artificial enzymes to replace their natural counterparts. Developing protocells that incorporate synthetic analogues of bio-derived signal transduction machineries will improve the robustness, stability, and versatility of protocells, and broaden their applications to highly strategic fields such as photocatalysis and fine chemicals production. 相似文献
956.
957.
Immune cell engineering is an active field of ongoing research that can be easily applied to nanoscale biomedicine as an alternative to overcoming limitations of nanoparticles. Cell membrane coating and artificial nanovesicle technology have been reported as representative methods with an advantage of good biocompatibility for biomimetic replication of cell membrane characteristics. Cell membrane-mediated biomimetic technique provides properties of natural cell membrane and enables membrane-associated cellular/molecular signaling. Thus, coated nanoparitlces (NPs) and artificial nanovesicles can achieve effective and extended in vivo circulation, enabling execution of target functions. While coated NPs and artificial nanovesicles provide clear advantages, much work remains before clinical application. In this review, first a comprehensive overview of cell membrane coating techniques and artificial nanovesicles is provided. Next, the function and application of various immune cell membrane types are summarized. 相似文献
958.
959.
The aim of this paper is to review recent literature regarding the determination of phosphorus species by ion chromatography (IC), and describe the implementation of new developments in sample treatment and ion chromatography methodology for the analysis of these compounds. Ion-exchange methods using both carbonate/hydrogencarbonate and hydroxide selective columns in combination with self-regenerating membrane and solid-phase-based suppressors enable determination of phosphate down to ppb levels. New technology, particularly on-line electrolytic hydroxide generators and electrolytic self-regenerating suppressor devices, has allowed the use of elution gradients in both carbonate/hydrogencarbonate and hydroxide selective systems, improving sensitivity and reducing total analysis time for samples containing phosphate together with other inorganic anions. In addition to a review of these developments, optimization and application of chromatographic methods using reversed stationary phases and cationic and/or zwitterionic surfactants is also discussed.The objective of most of the IC methods developed for phosphorus species is the determination of phosphate and total phosphorus. Therefore, sample treatment and separation conditions specifically developed for this purpose are also described. In addition, application of IC to the analysis of other inorganic (reduced and condensed) and organic (phytates, alkyl phosphate, and phosphonates) phosphorus species is discussed along with methodology and relevant applications in water analysis and other miscellaneous fields. 相似文献
960.
Shalova IN Asryants RA Sholukh MV Saso L Kurganov BI Muronetz VI Izumrudov VA 《Macromolecular bioscience》2005,5(12):1184-1192
The ability of synthetic polyanions to suppress thermo-aggregation of the oligomeric enzymes (glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, and aspartate aminotransferase) has been established. The ability of the polyanions to reduce the thermo-aggregation increased in the order poly(methacrylic acid) < poly(acrylic acid) < sodium poly(styrene sulphonate), which agreed well with the increase, in the same order, of the charge density of the chains. The lengthening of the chains, as well as the rise in their relative content, resulted in an increase of the ability to reduce thermo-aggregation, mentioned above. Complete prevention of the enzyme aggregation was achieved when highly charged polyanions of a relatively high degree of polymerization were used in a concentration sufficient to solubilize the protein. Complexing with the polyanions prevented thermo-aggregation of the enzymes, but not their thermo-denaturation. The adverse effect of the complexing polyanions on the catalytic activity was reduced by the addition of a synthetic polycation, which resulted in a significant reactivation (up to 40%) of the enzyme. The possibility of preventing the thermo-aggregation of enzyme molecules and then partly restoring the enzyme activity, appears to be of particular interest when studying the aggregation mechanism of proteins that are prone to form the amyloid structures responsible for the development of neurodegenerative diseases like Alzheimer's disease, bovine spongiform encephalopathy and Huntington disease. This finding can also be considered as an important step in the creation of artificial chaperones. 相似文献