Oligonucleotide-Based Approaches to Inhibit Dengue Virus Replication

Dengue fever is one of the most common viral infections affecting humans. It is an expanding public health problem, particularly in tropical and subtropical regions. No effective vaccine or antiviral therapies against Dengue virus (DENV) infection are available. Therefore, there is a strong need to develop safe and effective therapeutic strategies that can reduce the burden and duration of hospitalizations due to this life-threatening disease. Oligonucleotide-based strategies are considered as an attractive means of inhibiting viral replication since oligonucleotides can be designed to interact with any viral RNA, provided its sequence is known. The resultant targeted destruction of viral RNA interferes with viral replication without inducing any adverse effects on cellular processes. In this review, we elaborate the ribozymes, RNA interference, CRISPR, aptamer and morpholino strategies for the inhibition of DENV replication and discuss the challenges involved in utilizing such approaches.     

连续激光辐照ZnSe/MgF2/K9滤光片的透射特性研究

分析了连续激光辐照ZnSe/MgF2/K9滤光片引起透射特性的变化。在室温条件下,用波长0.632 8μm激光作为探测光束,测量了1.06μm连续激光辐照ZnSe/MgF2/K9滤光片温升引起薄膜折射率的改变,导致探测光束通过干涉滤光片后透过率的热致非线性变化。在光斑直径0.75mm条件下,测量了不同功率激光辐照ZnSe/MgF2/K9滤光片引起温升随时间的变化。在激光功率30W,辐照时间2.52s条件下,实验观测到ZnSe/MgF2/K9滤光片薄膜破坏温度约为90℃,辐照时间10s时干涉滤光片形成的薄膜龟裂形貌。     

激光干涉光刻法制作100 nm掩模

介绍了一种利用激光干涉光刻技术得到特征图形,并通过离子束刻蚀将图形转移到铬层上,从而获得掩模的方法。针对掩模透光率以及对干涉图形对比度可能产生影响的两个参数分别进行了数值仿真,从而证明此方法的可行性和参数的优化选择。自搭干涉光刻实验系统,用257 nm的激光光源实现光刻,得到特征尺寸为100 nm的图形,再经过离子束刻蚀,最终得到周期200 nm、线宽100 nm的掩模。     

同步光干涉方法对BEPCⅡ储存环束流的测量

利用同步光的可见光波段空间干涉方法,对北京正负电子对撞机储存环的束流横向截面垂直尺寸进行了测量。在同步模式下,测得束流横向截面垂直尺寸为155 μm,测量结果与理论值符合得很好。此方法避免了同步光衍射带来的测量误差,并使结果更精确,且对其它非相干小尺寸光源也同样适用。     

多相等离子体气动激励流动特性

等离子体激励器电极组相位不同便产生多相等离子体气动激励,建立了粒子图像测速仪流场参数测试系统,利用粒子图像测速仪技术,研究了非对称布局等离子体气动激励诱导空气流动特性,分析了多相等离子体气动激励对诱导空气流动速度的影响。结果表明：粒子图像测速仪流场测试系统能够准确地反映等离子体气动激励诱导空气流动的流场空间结构,等离子体气动激励诱导空气流动是平行于激励器的近壁面射流,多相等离子体气动激励能够增大等离子体气动激励诱导气流速度,或者使等离子体气动激励影响流场区域增大。粒子图像测速仪系统是深入研究等离子体气动激励的流场结构最佳的方式之一。     

多光束激光干涉光刻图样

利用电磁理论,研究了多光束激光干涉图样的产生原理,结合计算机数值模拟和相关实验结果,分析了干涉图样的影响因素。研究结果表明：多光束激光干涉图样可以看成是多组余弦分布的平行线条纹的叠加;相干光束的偏振方向、入射方向、光束间相差是干涉图样的重要影响因素,改变这些因素,余弦分布的平行线条纹的振幅、置、周期和方向发生变化,图样也随之变化。     

厄米-高斯电磁光束在杨氏干涉实验中的偏振度变化

基于随机电磁光束相干和偏振性的统一理论和交叉谱密度矩阵传输公式,将杨氏双缝干涉实验作为双缝衍射现象来处理,在考虑缝宽情况下,研究了厄米-高斯电磁光束在杨氏干涉实验中的偏振度变化,讨论了入射厄米-高斯电磁光束的偏振度、自相关和互相关长度、模阶数和双缝的遮拦比对观察屏上偏振度的影响。结果表明：观察屏上的偏振度随双缝面上偏振度和互相关长度的增加而增大,随自相关长度减小而增大;当模阶数为偶数时,观察屏轴上偏振度取极大值,模阶数为奇数时,取极小值。     

P波段微波注入反相器模数转换电路的响应

利用设计的三反相器模数（A/D）转换电路,开展了P波段微波注入实验。采用眼图观测法对电路的线性响应进行了有效测量,推导了实验线路中微波注入效率公式,利用一种实时温度检测电路来验证芯片工作状态的稳定性,并利用统计检验的方法分析了不同芯片及电路板等对实验数据获取的影响,从而保证了实验的有效性和可信度。重点研究了注入微波的幅值、频率、脉宽及重复频率等参数对反相器正常工作的影响。实验结果表明:当注入微波信号脉宽大于70 ns时,电路信号在微波脉冲结束后,相邻脉冲脉宽变化10%的非线性干扰功率阈值,比使电路信号噪声容限降低50%的功率阈值大4~6 dB,电路信号脉宽变化30%的功率阈值比脉宽变化10%的大2~3 dB,在功率小于32 dBm的实验中得到的最大非线性干扰为脉宽变化约40%。非线性干扰阈值随注入微波信号脉宽变化明显,拐点为40~70 ns。注入微波的重复频率对微波干扰阈值影响很小。     

Research on the optical fiber gas flowmeters based on intermodal interference

In this work, a self-heating type optical fiber flowmeter with high sensitivity was proposed. The core-offset fiber structures were employed to couple a part of signal light into the fiber cladding layer, and the other part of light still propagated in the core layer. The intermodal interference between the two parts of light happened when the cladding modes were coupled back into core layer. Meanwhile, the high power laser was also introduced into fiber to heat the silver film coated on the surface of the cladding layer. When the cool gas flow passed, the temperature of the sensor probe decreased due to the heat transfer process. Because of the thermo-optic effect in the fiber, interference spectrum could be shifted when the temperature was changed. The experimental results showed the resolution of the proposed sensor was 2×10⁻² m/s in the region of 0–8 m/s. The highest sensitivity could achieve 1537 pm/(m/s).