Analysis of multiplex PCR fragments with PMMA microchip

Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products.     

The most common friend first immunization

In this paper, a standard susceptible-infected-recovered-susceptible(SIRS) epidemic model based on the Watts–Strogatz(WS) small-world network model and the Barabsi–Albert(BA) scale-free network model is established, and a new immunization scheme — "the most common friend first immunization" is proposed, in which the most common friend's node is described as being the first immune on the second layer protection of complex networks. The propagation situations of three different immunization schemes — random immunization, high-risk immunization, and the most common friend first immunization are studied. At the same time, the dynamic behaviors are also studied on the WS small-world and the BA scale-free network. Moreover, the analytic and simulated results indicate that the immune effect of the most common friend first immunization is better than random immunization, but slightly worse than high-risk immunization. However,high-risk immunization still has some limitations. For example, it is difficult to accurately define who a direct neighbor in the life is. Compared with the traditional immunization strategies having some shortcomings, the most common friend first immunization is effective, and it is nicely consistent with the actual situation.     

Epidemic spreading on random surfer networks with infected avoidance strategy

In this paper, we study epidemic spreading on random surfer networks with infected avoidance(IA) strategy. In particular, we consider that susceptible individuals' moving direction angles are affected by the current location information received from infected individuals through a directed information network. The model is mainly analyzed by discrete-time numerical simulations. The results indicate that the IA strategy can restrain epidemic spreading effectively. However,when long-distance jumps of individuals exist, the IA strategy's effectiveness on restraining epidemic spreading is heavily reduced. Finally, it is found that the influence of the noises from information transferring process on epidemic spreading is indistinctive.     

一类具有媒体效应和追踪隔离的SIQR时滞传染病模型

建立了一类具有媒体效应和追踪隔离的SIQR时滞传染病模型,给出了模型的基本再生数R_{\mathrm{0}},并从稳定性、持久性和分支角度对该模型进行了理论分析和数值模拟。研究结果表明,由媒体报道产生的时滞\tau在各影响因子的临界值处出现Hopf分支。当\tau固定时,随着媒体的广泛报道,易感者对疾病信息认识的偏差程度\delta不断增加,模型由周期性振荡转为平衡;随着有效接触率最大削减作用{\beta }_{\mathrm{0}}和{\beta }_{\mathrm{00}}的不断增加,模型又由平衡状态转为周期性振荡。还研究了\delta,{\beta }_{\mathrm{0}},{\beta }_{\mathrm{00}}以及被追踪隔离者相关信息的媒体报道准确率\sigma对传染病发展的影响。结果表明,媒体对传染病信息的广泛报道以及提高报道信息的准确率可降低疾病传播,有利于控制传染病。     

一类具有非线性发生率的SIR传染病模型的全局稳定性

研究一类具有非线性发生率的SIR传染病模型.应用微分方程定性理论分别得到了该系统无病平衡点、地方病平衡点全局渐近稳定的充分条件,并进行了数值模拟.     

移动环境下网络病毒传播模型及其稳定性研究

考虑网络节点的随机移动, 基于平均场理论 提出一个移动环境下网络病毒传播的数学模型, 利用微分动力学系统理论研究了病毒传播行为. 研究表明, 当病毒基本再生数R₀ ≤ 1时, 网络中病毒逐渐消除, 系统的无病毒平衡点全局渐进稳定; 当R₀ > 1时, 网络中病毒持续存在, 系统的地方病平衡点全局渐进稳定.通过仿真验证了所得结论的正确性.     

潜伏期和染病期均具有传染性的媒介传染病模型的全局稳定性分析

讨论潜伏期和染病期均具有传染性的媒介传染病模型.得到模型基本再生数的表达式,证明了当基本再生数小于1时,无病平衡点是全局渐近稳定的,此时疾病消亡;当基本再生数大于1时,无病平衡点是不稳定的,系统存在全局渐近稳定的地方病平衡点,此时,疾病将在人群中持续存在,数值模拟验证了理论结果.     

Dynamic analysis of an SIR epidemic model with state dependent pulse vaccination

An SIR epidemic model with state dependent pulse vaccination is proposed in this paper. Using the Poincaré map, the differential inequality and the method of qualitative analysis, we prove the existence and the stability of positive order-1 or order-2 periodic solution for this model. Moreover, we show that there is no periodic solution with order larger than or equal to three. Numerical simulations are carried out to illustrate the feasibility of our main results and the suitability of state dependent pulse vaccination is also discussed.     

Epidemic Spread in Networks Induced by Deactivation Mechanism

We have studied the topology and epidemic spreading behaviors on the networks in which deactivation mechanism and long-rang connection are coexisted. By means of numerical simulation, we find that the clustering coefficient C and the Pearson correlation coefficient r decrease with increasing long-range connection μ and the topological state of the network changes into that of BA model at the end （when μ = 1）. For the Susceptible-Infect-Susceptible model of epidemics, the epidemic threshold can reach maximum value at μ = 0.4 and presents two different variable states around μ= 0.4.     

一类传染病模型的全局解

研究一个具时滞和扩散的传染病模型,该模型数学上可用非线性反应扩散方程组描述,重点研究了该方程组解的定性性质.用上下解的方法证明了该方程组全局解的存在唯一性.