Monitoring membrane rafts in colorectal cancer cells by means of correlative fluorescence electron microscopy (CFEM)

Detergent-resistant membrane (DRM) rafts have been shown to play a pivotal role in regulating key cell biological processes, such as signal transduction, cellular transport and cell survival. The fine structure of membrane rafts are studied using various different imaging approaches and the outcomes are largely dependent on the detection methodology applied. All these microscopy techniques which employ light-, laser- and photon-optics, electrons as well as atomic force probing are characterized on their turn by their strengths and limitations for membrane raft identification. This explains in part the diversity of definitions available to describe these peculiar membrane structures. We present herewith an alternative and uncomplicated microscopy tool to study fluorescently labelled DRMs with information at the transmission electron microscopical level of the same cell, enabling us to obtain a snapshot of the morpho-functional relationships between the cell's interior and DRMs. The proposed approach of correlative fluorescence electron microscopy (CFEM) can therefore be considered as an additional alternative imaging approach to unravel DRM structure–function relationships from micro- to nanometre length scales, from the cell to the molecule.     

夹芯圆柱壳在水下爆炸载荷作用下的抗冲击特性

采用声固耦合方法对夹芯圆柱壳和等质量的普通圆柱壳在爆炸载荷作用下的应变、速度和加速度进行有限元计算。结果表明:夹芯防护层对爆炸冲击波可起到较好的衰减作用,即通过芯层的塑性变形,耗散了冲击过程中产生的大部分能量,对里面的圆柱壳体起到较好的保护作用,由于夹芯防护层的存在,与等质量的普通圆柱壳相比,夹芯圆柱壳能够承受更强的爆炸冲击波,降低结构的整体变形。     

爆炸容器内冲击波系演化及壳体响应的数值研究

对中心装药爆炸后冲击波的产生、传播和壳体动态响应全过程进行了数值研究。认为RDX瞬时爆炸 ,爆炸近场采用自相似解 ;冲击波传播和波系演化采用PPM (the Piecewise Parabolic Method)格式求解Eu ler方程 ;壳体响应采用有限元方法求解拉氏坐标系下由虚功原理得到的动力学方程。壳体内壁面边界条件分别采用强耦合和弱耦合方法处理。结果表明 :(1)当装药量相同时 ,薄壁壳体振型比厚壁壳体复杂得多 ,振幅也大 ;(2 )当装药量不同 ,壳体厚度相同时 ,爆炸场冲击波的演化过程不同 ;(3)对少量装药 ,产生的冲击波强度低 ,壳体变形小 ,是否考虑内边界运动 ,对计算结果的影响不大 ;(4 )在本文条件下 ,爆炸容器封头顶点所受的载荷最大 ,是最易发生破坏的地方 ,侧壁与爆点所在横截面的交线 ,也易破坏。     

侧向不均匀冲击下环向加筋圆柱壳的动力响应

针对空投鱼雷入水冲击问题,研究了环向加筋圆柱壳在侧向不均匀冲击载荷作用下的动力响应。考虑了壳体剪切变形、转动惯性以及加筋肋骨的拉伸、弯曲和剪切变形,运用Hamilton变分原理推导出了运动方程,并且采用一种半解析有限差分法进行了求解。分析了加肋数目、截面宽度比及加筋形式等因素对结构动力响应的影响。     

Highly selective coextraction of rhodamine B and dibenzyl phthalate based on high‐density dual‐template imprinted shells on silica microparticles

A simple one‐pot approach based on molecularly imprinted polymer shells dispersed on the surface of silica for simultaneous determination of rhodamine B and dibenzyl phthalate (DBzP) has been developed. Highly dense molecularly imprinted polymer shells were formed in the mixture of acetonitrile and toluene by the copolymerization of methacrylic acid and ethylene glycol dimethacrylate, as well as two templates, rhodamine B and dibenzyl phthalate, directed by the vinyl end groups functional monolayer at surface silica microspheres after 3‐methacryloxypropyl trimethoxysilane modification. The obtained imprinted polymer shells showed large average pore diameter (102.5 nm) and about 100 nm shell thickness. The imprinted particles also showed high imprinting factor (α_RhB = 3.52 and α_DBzP = 3.94), rapid binding kinetics, and excellent selective affinity capacity for rhodamine B and dibenzyl phthalate containing another three competitors in mixed solution. Moreover, the imprinted particles coupled with ultra high performance liquid chromatography was successfully applied to simultaneous analysis of rhodamine B and dibenzyl phthalate in two spiked beverage samples with average recoveries in the range of 88.0−93.0% for rhodamine B and 84.0–92.0% for dibenzyl phthalate with the relative standard deviation lower than 5.1%.     

圆柱壳轴向流固冲击屈曲与塑性失效的实验研究

通过对三种不同径厚比圆柱壳进行的流固冲击实验，分析了它们在流固冲击载荷下的动力响应特点，并与相应的高速撞击与静力屈曲实验作了简单的比较，分析了它们之间的异同点。同时文中对极值冲击倒塌特性进行了详细分析     

用于旋转壳分析的广义样条子域法

本文以非等距Ｂ＿３样条函数为基函数分别建立了旋转壳子午向和环向形函数，其中环向形函数满足周期性条件。采取先子午向插值后环向插值的方式得到旋转壳广义样条子域位移模式。该模式可沿子午向与环向各结线任意设取结点，亦即不同环线可以有不同的结点数，从而大大提高了样条子域法的灵活性，拓宽了其应用范围。     

Thermoelastoplastic axisymmetric stress-strain state of laminated orthotropic shells

A method is proposed for the numerical analysis of the thermoelastoplastic stress-strain state of laminated shells of revolution, made of isotropic and orthotropic materials, under axisymmetric loading. The method is based on the Kirchhoff-Love hypotheses for a layer stack, the theory of deformation along paths of small curvature for isotropic materials, and Hills theory of flow with isotropic hardening for orthotropic materials. The problem is solved by the method of successive approximations. A numerical example is given.__________Translated from Prikladnaya Mekhanika, Vol. 40, No. 12, pp. 84–91, December 2004.     

Non-linear behaviour of free-edge shallow spherical shells: Effect of the geometry

Non-linear vibrations of free-edge shallow spherical shells are investigated, in order to predict the trend of non-linearity (hardening/softening behaviour) for each mode of the shell, as a function of its geometry. The analog for thin shallow shells of von Kármán's theory for large deflection of plates is used. The main difficulty in predicting the trend of non-linearity relies in the truncation used for the analysis of the partial differential equations (PDEs) of motion. Here, non-linear normal modes through real normal form theory are used. This formalism allows deriving the analytical expression of the coefficient governing the trend of non-linearity. The variation of this coefficient with respect to the geometry of the shell (radius of curvature R, thickness h and outer diameter 2a) is then numerically computed, for axisymmetric as well as asymmetric modes. Plates (obtained as R→∞) are known to display a hardening behaviour, whereas shells generally behave in a softening way. The transition between these two types of non-linearity is clearly studied, and the specific role of 2:1 internal resonances in this process is clarified.     

Axisymmetric thermoelastoplastic stress-strain state of flexible laminated transversely isotropic shells

The paper presents a technique for numerical analysis of the elastoplastic stress-strain state of flexible laminated shells of revolution made of isotropic and transversely isotropic materials and subjected to axisymmetric loading and heating. The technique is based on the Kirchhoff-Love hypotheses for the whole laminate. The deformation of the isotropic materials is described using the theory of deformation along paths of small curvature. The deformation of the transversely isotropic material is described using the flow theory with isotropic hardening. The process of loading is divided into steps at each of which the stress-strain state is determined by the method of successive approximations. A numerical example is given __________ Translated from Prikladnaya Mekhanika, Vol. 42, No. 12, pp. 76–86, December, 2006.