Characterization of bovine serum albumin adsorption on chromium and AISI 304 stainless steel, consequences for the Pseudomonas fragi K1 adhesion

Adsorption of BSA on the surface of chromium and 304 stainless steel, has been characterized by Contact Angle Measurements, X-ray Photoelectron Spectroscopy (XPS) and Infrared Reflection Absorption Spectroscopy (IRRAS). Bacterial adhesion has been tested and compared on these two materials before and after pre-conditioning the surface with BSA. Chromium and stainless steel surfaces, when covered by a natural oxide layer, exhibit different energetic characteristics as shown by their γ_s^- and γ_s^LW respective values. These data vary upon immersion in BSA solutions, tending towards common values for duration of immersions. After immersion in BSA solutions, the evolution of the N 1s XPS signal, specific for the BSA, suggests that the surface is nearly saturated in a few minutes. Longer times of immersion only lead to a re-ordering of the adsorbed layer. Immersion tests in dilute BSA solutions (0.01 g/l) enabled us to make clear a higher reactivity of chromium towards the protein compared to stainless steel. These differences are cancelled at higher BSA concentrations (1 g/l). IRRAS spectra of BSA adsorbed on the two substrates demonstrated the appearance of amide I and amide II bands with small shifts and intensity variations supporting orientation changes of the protein when the concentration or immersion time varies. A model for the building up of the BSA layer is proposed, which accounts for these data. Chromium and stainless steel surfaces, also have different behaviours towards adhesion of Pseudomonas fragi K1, whereas surfaces that are pre-conditioned by BSA behave in a similar way. The overall number of adherent bacteria is decreased on stainless steel, whereas it is hardly affected on chromium. On both surfaces, the fraction of viable cells is increased.     

AISI304钢表面低电压等离子体基离子注入层摩擦磨损性能研究

采用高频低电压等离子体浸没离子注入（ＨＬＰＩＩＩ）技术对ＡＩＳＩ３０４不锈钢表面进行了氮离子注入处理;用球－盘摩擦磨损试验机考察了注入处理后钢表面改性层的摩擦磨损性能;用扫描电子显微镜（ＳＥＭ）、俄歇电子能谱仪（ＡＥＳ）和Ｘ射线衍射（ＸＲＤ）考察了改性层的相组成、Ｎ元素的深度分布及磨损机理。研究结果表明：ＨＬＰＩＩＩ处理能够显著提高样品的摩擦学性能,且其摩擦学性能对温度有较大的依赖性,４００℃下处     

Effect of silver on antibacterial properties of stainless steel

The microstructural variation and antibacterial properties of the AISI 304 stainless steel containing silver (Ag) element have been investigated by means of optical microscopy (OM), grazing incidence X-ray diffractometry (GIXRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). Furthermore, the antibacterial testing was performed according to JIS Z2801:2000 specification. As the alloy contained Ag elements, the microstructure of the alloys was a mixture of (α + γ + Ag-rich compound)-phases. The amounts of α phase and Ag-rich compound increased as Ag contents increased. The Ag-rich compound has FCC structure with the lattice parameter a = 0.251 nm. No precipitates were found within the matrix and grain boundaries in the present alloys after SHT. Moreover, when the alloy is added to Ag element, antibacterial property was seen obvious against E. coli. It has an AR nearly of 100%.     

45~#钢/GCr15钢摩擦副的滚动磨损特性研究

通过对液压式试验台夹具进行改造 ,研制了往复滚动试验装置 ,以实现对运动位移和表面摩擦力的精确控制 .利用该装置考察了表面摩擦力变化对 45 #钢 / GCr15钢摩擦副滚动磨损特性的影响 .结果表明 :随着表面摩擦力的增加 ,钢的滚动磨损明显加剧 ,磨损机制亦发生变化 .