TILLING技术的最新进展及其在辐射诱变育种中的应用前景

定向诱导基因组局部突变技术(Targeting Induced Local Lesions IN Genomes, 简称TILLINC)是一种全新的、高通量和低成本反向遗传学研究方法。近年来, 随着突变筛选技术的革新, TILLING技术平台日趋多元化, 使得TILLING技术的操作更为简单﹑快速, 并广泛应用于作物育种研究领域。简要介绍了TILLING技术平台的最新发展动态, 并初步探讨了将辐射诱变处理与TILLING高通量筛选相结合在诱变育种中的应用前景。To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by 12C6+heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to 12C6+ heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.     

450 kV数字化X光照相诊断系统

介绍了450 kV数字化X光照相诊断系统的研制,该系统由450 kV脉冲X光机和抗辐照X光CCD相机组成。450 kV脉冲X光机输出的X射线脉冲半高宽为40 ns,1 m处剂量为5.210-6 C/kg,焦斑2~5 mm,2.5 m处可穿透20 mm的Fe。抗辐照X光CCD相机接收X光能谱范围为10~450 keV,有效成像面积最高可达100 mm100 mm,空间分辨率最高可达3 lp/mm,具有很强的抗电磁干扰能力。进行爆轰实验时在总防护为40 mm铝板的情况下,450 kV数字化X光照相诊断系统的照相视场可视范围超过70 mm,空间分辨率优于1.2 lp/mm。     

稳定律吸引场中学生化的Hanson Russo型增量定理

该文通过建立关于部分和学生化增量的大偏差不等式, 在稳定律吸引场中证得了学生化的Hanson Russo型增量定理.     

微波消解-电感耦合等离子体发射光谱筛选法对电子电气产品塑料部件中有机锡的测定

建立了微波消解-电感耦合等离子体原子发射光谱(ICP-AES)筛选法测定电子电气产品塑料部件中有机锡的方法.结果表明:采用5 mL硝酸、1 mL 30%双氧水和1 mL氟硼酸消解体系,在190 ℃下消解30 min可使绝大多数塑料样品获得较好的消解效果,选取聚氯乙烯(PVC)、聚苯乙烯(PS)和丙烯腈-丁二烯-苯乙烯(ABS)3种典型空白塑料基质进行标准添加回收实验,回收率为88%～102%,相对标准偏差(RSD)小于3%.实际阳性样品的测定结果显示,建立的方法与GC-MS方法的测定结果吻合.该方法简单快速、灵敏度高、精密度好,可满足实际电子电气产品中有机锡筛选的需要.