The signal-to-noise level of light emitting diode (LED) fluorimetry using a liquid-core-waveguide (LCW)-based microfluidic capillary electrophoresis system was significantly enhanced using a synchronized dual wavelength modulation (SDWM) approach. A blue LED was used as excitation source and a red LED as reference source for background-noise compensation in a microfluidic capillary electrophoresis (CE) system. A Teflon AF-coated silica capillary served as both the separation channel and LCW for light transfer, and blue and red LEDs were used as excitation and reference sources, respectively, both radially illuminating the detection point of the separation channel. The two LEDs were synchronously modulated at the same frequency, but with 180°-phase shift, alternatingly driven by a same constant current source. The LCW transferred the fluorescence emission, as well as the excitation and reference lights that strayed through the optical system to a photomultiplier tube; a lock-in amplifier demodulated the combined signal, significantly reducing its noise level. To test the system, fluorescein isothiocyanate (FITC)-labeled amino acids were separated by capillary electrophoresis and detected by SDWM and single wavelength modulation, respectively. Five-fold improvement in S/N ratio was achieved by dual wavelength modulation, compared with single wavelength modulation; and over 100-fold improvement in S/N ratio was achieved compared with a similar LCW-CE system reported previously using non-modulated LED excitation. A detection limit (S/N = 3) of 10 nM FITC-labeled arginine was obtained in this work. The effects of modulation frequency on S/N level and on the rejection of noise caused by LED-driver current and detector were also studied. 相似文献
Using a photoelastic modulator‐based novel set‐up, the electric field‐induced in‐plane birefringence and the optical rotatory power (ORP) were measured of an antiferroelectric liquid crystalline compound (12OF1M7) in its various phases using 30 µm homeotropic cells. Some specific signatures of the in‐plane birefringence and of the ORP for the various phases are being established. A relatively small threshold field is needed for the unwinding process of the antiferroelectric phase with a unit cell of four layers [SmCA*(1/2)] compared with that for two layers [SmCA*(0)]. On application of the electric field on the high temperature side of the SmCA*(1/2) phase (80.1–81.5°C), a field‐induced phase transition is shown to occur directly to the SmC* phase, whereas on the lower temperature side (79.4–80.1°C) the transition takes place to SmC* via the SmCA*(1/3) phase. The in‐plane birefringence exhibits a critical power law dependence for the SmC*–SmA transition. The ORP changes sign within the temperature range of the phase with a unit cell of three layers, reflecting a change in the handedness during this phase. Using tilted conoscopy, the results for the biaxiality and the apparent tilt angle for a smectic liquid crystal with a tilt angle greater than 18° in the ferroelectric phase are reported. The biaxiality implies the difference in the refractive indices between the two minor axes of the refractive index ellipsoid. The optical transmittance at visible and IR wavelengths for free‐standing films reveal characteristic reflection bands for these phases. The modulated structures of the reflected bands appear just above the SmCA* phase and below SmCA*(1/3); these are possibly due to an easy deformation of the phase by the surfaces. 相似文献
The laser breakdown time-of-flight mass spectrometry was developed and applied to detect the breakdown patterns of hydrocarbons that are significant to improve the detectability of heavy metals compounded in various mixtures such as particles and hydrocarbons. The laser wavelength dependence of this method was evaluated using 1064, 532, and 266 nm laser outputs. Breakdown processes using 1064 and 532 nm outputs showed less interference of fragmentation for the detection of various heavy metals. The method was applied to various hydrocarbons and it showed the possibility of categorization of hydrocarbon groups using the pattern of breakdown products. 相似文献
Abstract In this article, two static routing algorithms have been proposed and compared to some of the existing algorithms on the basis of blocking probability. The two proposed static routing and wavelength assignment algorithms reduce the blocking probability to maximize the utilization of the network. All of these algorithms are analyzed and compared with four wavelength assignment schemes, which are first-fit, random, most used, and least used. It is shown that our proposed static algorithms give the best performance for first-fit wavelength assignment and most used wavelength assignment strategies with reduced complexity. For least used wavelength assignment and random wavelength assignment, 1 fixed and 2 alternate routing algorithm gives the lowest blocking probability. 相似文献
Grooming uniform all‐to‐all traffic in optical (SONET) rings with grooming ratio C requires the determination of a decomposition of the complete graph into subgraphs each having at most C edges. The drop cost of such a grooming is the total number of vertices of nonzero degree in these subgraphs, and the grooming is optimal when the drop cost is minimum. The determination of optimal C‐groomings has been considered for , and completely solved for . For , it has been shown that the lower bound for the drop cost of an optimal C‐grooming can be attained for almost all orders with 5 exceptions and 308 possible exceptions. For , there are infinitely many unsettled orders; especially the case is far from complete. In this paper, we show that the lower bound for the drop cost of a 6‐grooming can be attained for almost all orders by reducing the 308 possible exceptions to 3, and that the lower bound for the drop cost of a 7‐grooming can be attained for almost all orders with seven exceptions and 16 possible exceptions. Moreover, for the unsettled orders, we give upper bounds for the minimum drop costs. 相似文献
An all-to-all routing in a graph G is a set of oriented paths of G, with exactly one path for each ordered pair of vertices. The load of an edge under an all-to-all routing R is the number of times it is used (in either direction) by paths of R, and the maximum load of an edge is denoted by . The edge-forwarding index is the minimum of over all possible all-to-all routings R, and the arc-forwarding index is defined similarly by taking direction into consideration, where an arc is an ordered pair of adjacent vertices. Denote by the minimum number of colours required to colour the paths of R such that any two paths having an edge in common receive distinct colours. The optical index is defined to be the minimum of over all possible R, and the directed optical index is defined similarly by requiring that any two paths having an arc in common receive distinct colours. In this paper we obtain lower and upper bounds on these four invariants for 4-regular circulant graphs with connection set , . We give approximation algorithms with performance ratio a small constant for the corresponding forwarding index and routing and wavelength assignment problems for some families of 4-regular circulant graphs. 相似文献
A compact 64‐channel hybrid demultiplexer based on silicon‐on‐insulator nanowires is proposed and demonstrated experimentally to enable wavelength‐division‐multiplexing and mode‐division‐multiplexing simultaneously in order to realize an ultra‐large capacity on‐chip optical‐interconnect link. The present hybrid demultiplexer consists of a 4‐channel mode multiplexer constructed with cascaded asymmetrical directional‐couplers and two bi‐directional 17 × 17 arrayed‐waveguide gratings (AWGs) with 16 channels. Here each bi‐directional AWG is equivalent as two identical 1 × 16 AWGs. The measured excess loss and the crosstalk for the monolithically integrated 64‐channel hybrid demultiplexer are about ‐5 dB and ‐14 dB, respectively. Better performance can be achieved by minimizing the imperfections (particularly in AWGs) during the fabrication processes.