基于微流控技术的磁免疫荧光法在EB病毒检测中的应用

EB病毒(Epstein-Barr virus,EBV)的早期诊断能够降低患者发生重大疾病的风险.临床上常用的EBV抗体的检测方法存在耗时长、试剂消耗大和效率低等缺点.相比于传统的检测方法,微流控(microfluidics)技术具有高通量、试剂消耗少,污染少和自动化程度高等优点,磁免疫荧光技术具有检测效率高、信号强等...     

Can preoperative diffusion-weighted MRI predict postoperative hepatic insufficiency after curative resection of HBV-related hepatocellular carcinoma? A pilot study

Liver fibrosis determines the functional liver reserve. Several studies have reported that the apparent diffusion coefficient (ADC) values of diffusion-weighted magnetic resonance imaging (DW-MRI) can assess liver fibrosis. We investigated whether DW-MRI predicts postoperative hepatic insufficiency and liver fibrosis in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Twenty-six patients with HBV-related HCC who received preoperative DW-MRI on a 3-T MRI system were enrolled between July and December 2008. ADC values were measured twice by two observers. Three “b values” were used: 50, 400 and 800 s/mm². Postoperative hepatic insufficiency was defined as persistent hyperbilirubinemia (total bilirubin level >5 mg/dl for more than 5 days after surgery) or postoperative death without other causes. The mean age (21 men and 5 women) was 51.4 years. Three patients experienced postoperative hepatic insufficiency. liver stiffness measurement predicted postoperative hepatic insufficiency, advanced fibrosis (F3–4), and cirrhosis significantly [area under the receiving operator characteristic curve (AUROC)=0.942, 0.771 and 0.818, respectively, with P=.047, 0.048 and 0.006, respectively]; ADC values of DW-MRI, however, did not (AUROC=0.797, 0.648 and 0.491, respectively, with P=.100, 0.313 and 0.938, respectively). Reliability of ADC values between right and left hepatic lobes (ρ=0.868 and ρ=0.910 in the first and second measures of Observer A; ρ=0.865 and ρ=0.831 in the first and second measures of Observer B) was high and the intra- and interobserver reliability (ρ=0.958 in observer A and ρ=0.977 in observer B; ρ=0.929 in the first measure and ρ=0.978 in the second measure between the two observers) were high. All reliability was significant (P<.001). Our results suggest that DW-MRI on a 3-T MRI system is not suitable for predicting postoperative hepatic insufficiency, advanced liver fibrosis, and cirrhosis in patients with HBV-related HCC, despite significantly high reliability.     

Synthesis and Antiviral Properties of Camphor-Derived Iminothiazolidine-4-Ones and 2,3-Dihydrothiazoles

A set of heterocyclic products was synthesized from natural (+)-camphor and semi-synthetic (−)-camphor. Then, 2-Imino-4-thiazolidinones and 2,3-dihydrothiazoles were obtained using a three-step procedure. For the synthesized compounds, their antiviral activity against the vaccinia virus and Marburg virus was studied. New promising agents active against both viruses were found among the tested compounds.     

RT-PCR技术在凡纳滨对虾健康苗种培育中的应用

应用PCR和RT-PCR技术对WSSV、IHHNV和TSV这3种对虾病毒在凡纳滨对虾育苗过程中各个环节进行了检测,防止亲虾、卵、无节幼体、仔虾、亲虾饵料、丰年虫、水体等诸环节受到以上3种病毒携带或感染,保证培育出的凡纳滨对虾仔虾是高健康的虾苗,为凡纳滨对虾健康养殖的可持续发展提供一条可行的途径．     

基于液相色谱-质谱代谢组学方法研究中药定喘汤对呼吸道合胞病毒感染的疗效

呼吸道合胞病毒(RSV)容易引发下呼吸道感染,尤其是小儿毛细支气管炎.中药定喘汤用于RSV感染的治疗在中国有着广泛的临床实践基础.由于中药体系的复杂性,其治疗机制和主要药效成分还不够明确.采用代谢组学方法分析中药药效,可以为传统中药治疗提供现代科学论证.该研究对大鼠各干预组分别采用中药定喘汤全方,宣、降、清分解剂以及利...     

体积排阻色谱法定量检测9种型别人乳头瘤病毒原液中病毒样颗粒

据统计,5％以上的人类癌症由人乳头瘤病毒(HPV)导致.HPV疫苗的使用,尤其是多价HPV疫苗的使用,可有效预防HPV感染和肿瘤的发生.例如,9价HPV疫苗可有效预防90％以上HPV相关癌前病变.人乳头瘤病毒样颗粒(VLP)是HPV疫苗的唯一抗原.VLP由360份衣壳蛋白L1组成.VLP的含量测定对HPV原液和HPV疫...     

一类时滞分数阶计算机病毒模型的稳定性研究

本文研究一类改进的时滞分数阶计算机病毒模型正平衡点的稳定性问题.利用线性化方法和拉普拉斯变换获得模型对应的线性化系统的特征方程,通过讨论特征方程的根以及横截条件研究时滞和正平衡点稳定性之间的关系,推导了Hopf分支出现时时滞临界值的计算公式,并选择恰当的系统参数进行数值模拟以验证理论分析的合理性.     

Synthesis of Pyrimidine Conjugates with 4-(6-Amino-hexanoyl)-7,8-difluoro-3,4-dihydro-3-methyl-2H-[1,4]benzoxazine and Evaluation of Their Antiviral Activity

A series of pyrimidine conjugates containing a fragment of racemic 7,8-difluoro-3,4-dihydro-3-methyl-2H-[1,4]benzoxazine and its (S)-enantiomer attached via a 6-aminohexanoyl fragment were synthesized by the reaction of nucleophilic substitution of chlorine in various chloropyrimidines. The structures of the synthesized compounds were confirmed by ¹H, ¹⁹F, and ¹³C NMR spectral data. Enantiomeric purity of optically active derivatives was confirmed by chiral HPLC. Antiviral evaluation of the synthesized compounds has shown that the replacement of purine with a pyrimidine fragment leads to a decrease in the anti-herpesvirus activity compared to the lead compound, purine conjugate. The studied compounds did not exhibit significant activity against influenza A (H1N1) virus.     

糖芯片研究

糖芯片是继基因芯片、蛋白质芯片、组织芯片等之后发展起来的一种很有前景的生物检测技术,具有检测样品用量少、特异性高、高通量等优点,可以大大提高糖化学研究的效率。本文介绍利用共价结合法和非共价吸附法制备二维糖芯片,利用聚合反应制备三维凝胶芯片以及糖芯片在凝集素功能研究、病毒转染机制研究、细菌检测和免疫学研究等方面的应用,最后对糖芯片今后的发展进行了展望。