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冷态德士古气化炉流场与停留时间分布的研究 总被引:5,自引:1,他引:5
测定了德士古气化炉的冷态速度分布和停留时间分布。结果表明:德士古气化炉内在轴向2.5~3.0倍直径范围内存在回流,最大回流量为射流量的3.5倍左右;在平均停留时间之前,已有50%左右的物料逸出炉外。本文指出,德士古气化炉中存在的问题,如结渣、耐火砖寿命短、有效气体成分偏低均与炉内流场有关。 相似文献
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Summary For the title compounds 4,4-DADPM, MOCA, 3,3-DCB, 4-ADP and 4-ADPA listing on the EPA priority pollutant list, an analytical practicable, reliable, reproducible and sensitive procedure is required. Therefore a new method has been developed for the routine determination of these toxic aromatic amines in urine at the ppb level. The quantitative determination of amines is a suitable procedure of occupationally exposed persons. Urine sample preparation is done using simple liquid-liquid extraction followed by a precolumn enrichment (PRP1-material; Hamilton). Breakthrough measurements were done using an enrichment column packed with PRP1 material. The capacities of the studied amines ranged from 21.9mg/g to 96.6mg/g, while influent concentrations differed from 28.3mg/l to 332.0mg/l. The advantages of electrochemical detection regarding to selectivity and sensitivity are clearly indicated in this paper. Separation has been achieved applying reversed-phase-high-performance-liquid chromatography (LiChrosorb RP 18/5m) followed by electrochemical or UV-detection. The detection limits employing an electrochemical detector at a potential of 1 V range from 2.2ng to 12.1ng. UV detection at 254 nm and 280 nm is about 10–100 times less sensitive. Recoveries from spiked water samples at the 5ppb levels were 75% to 96% respectively. The standard deviation of the developed procedure varies from 5.3% to 14%. Day-to-day repeatability is good. 相似文献
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桑蚕丝素蛋白初始结构对其矿化作用的影响 总被引:4,自引:0,他引:4
以碱金属离子诱导桑蚕丝素蛋白溶液发生构象转变, 研究了蛋白质初始结构对其矿化作用的影响. FT-IR, XRD和SEM等测试结果显示, 未经任何处理的桑蚕丝素蛋白溶液矿化后形成片状复合物, 其无机相以二水磷酸氢钙(DCPD)为主; 而经过K+和Na+金属离子处理后, 桑蚕丝素溶液的结构由无规线团/螺旋构象向β-折叠发生转变, 矿化后成纤维状, 并相互结合呈现纳米级的三维多孔结构, 其无机相以热力学稳定的羟基磷灰石(HA)为主. 可以认为, 丝素蛋白结构转化为较伸展的β-折叠后, 使得更多的亲水基团暴露在外面, 在丝素蛋白分子不断凝聚成纤过程中, HA结晶快速生长并附着在这些微纤上, 最终形成纤维状的丝素蛋白/HA复合物. 该结果为阐明蛋白质的生物矿化过程及其调控机理提供了理论依据, 同时可以从矿化复合物的形成来反映这些微量元素可能对骨组织形成的影响, 为临床骨组织的修复提供一定的参考. 相似文献
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ICP-MS (inductively coupled plasma mass spectrometry) is shown as a very sensitive method for quantitative determination of Th and U concentration and excretion analysis in urine without any sample pretreatment. The current standard method for incorporation monitoring applies alpha-spectrometry, a very tedious and time consuming technique. ICP-MS offers an attractive alternative for monitoring of thorium and uranium body burdens in occupationally exposed subjects and also larger groups of the general population. A limit of determination of 0.5 ng/L in aqueous solutions and 1 ng/L in urine samples for both elements was achieved, with a precision of about ± 10% in the concentration range of appr. 10 ng/L. Due to the lack of a suitable reference material, the accuracy of the method was tested by comparing some of the results with those obtained by -spectrometry, especially for U. There was a sufficient agreement on both results. 相似文献
78.
ZNF191 (243-368), a new human zinc finger protein, probably relates to some hereditary diseases and cancers, To obtain adequate amount of ZNF191(243-368) for the study of its property, structure and function, three different expression systems of inclusion-body, glutathione S-transferase (GST), and hexahistidine (6 × His) were used and compared. Among these systems, the expression level of ZNFI91(243-368) was increased in inclusion body system under a higher isopropylthio-β-D-galactoside (IPTG) concentration, but the non-target proteins were also increased more, which made its purification more difficult and the yield lower. The expression of His-tag fusion protein was almost not affected by IPTG concentration, temperature and inducing time. At a high IPTG concentration the highest expression yield for GST fusion protein was obtained. And the fusion proteins can be partially purified by a single affinity chromatography step. The fusion protein systems show advantages for expression of these proteins. 相似文献
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Binal N. Shah 《Thermochimica Acta》2006,443(1):78-86
Protein crystals are usually grown in multi-component aqueous solutions containing salts, buffers and other additives. To measure the X-ray diffraction data of the crystal, crystals are rapidly lowered to cryogenic temperatures. On flash cooling, ice frequently forms affecting the integrity of the sample. In order to eliminate this effect, substances called cryoprotectants are added to produce a glassy (vitrified) state rather than ice. Heretofore, the quantity of cryoprotectant needed to vitrify the sample has largely been established by trial and error. In this study, differential scanning calorimetry (DSC) was used to measure the melting (Tm), devitrification (Td) and glass transition (Tg) temperatures of solutions with a range of compositions typical of those used for growing protein crystals, with the addition of glycerol as cryoprotectant. The addition of cryoprotectant raises the Tg and lowers the Tm of bulk solution thereby decreasing the cooling rates required for vitrification of protein crystals. The theoretical Tg value was calculated using the apparent volume fraction using the Miller/Fox equation extended for multi-component systems. The experimental values of Tg were within approximately ±4% of that predicted by the model. Thus, the use of the model holds the promise of a rational method for the theoretical determination of the composition of cryoprotectant requirement of protein crystallization solutions. 相似文献