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21.
Chlorella is one of the most well-known microalgal genera, currently comprising approximately a hundred species of single-celled green algae according to the AlgaeBase. Strains of the genus Chlorella have the ability to metabolize both inorganic and organic carbon sources in various trophic modes and synthesize valuable metabolites that are widely used in many industries. The aim of this work was to investigate the impact of three trophic modes on the growth parameters, productivities of individual cell components, and biochemical composition of Chlorella sorokiniana, Chloroidium saccharofilum, and Chlorella vulgaris cells with special consideration of protein profiles detected by SDS-PAGE gel electrophoresis and two-dimensional gel electrophoresis with MALDI-TOF/TOF MS. Mixotrophic conditions with the use of an agro-industrial by-product stimulated the growth of all Chlorella species, which was confirmed by the highest specific growth rates and the shortest biomass doubling times. The mixotrophic cultivation of all Chlorella species yielded a high amount of protein-rich biomass with reduced contents of chlorophyll a, chlorophyll b, carotenoids, and carbohydrates. Additionally, this work provides the first information about the proteome of Chloroidium saccharofilum, Chlorella sorokiniana, and Chlorella vulgaris cells cultured in molasses supplementation conditions. The proteomic analysis of the three Chlorella species growing photoheterotrophically and mixotrophically showed increased accumulation of proteins involved in the cell energy metabolism and carbon uptake, photosynthesis process, and protein synthesis, as well as proteins involved in intracellular movements and chaperone proteins. 相似文献
22.
Pawe Mateusz Nowak Maria Klag Gabriela Kzka Magorzata Gob Micha Wo
niakiewicz 《Molecules (Basel, Switzerland)》2022,27(15)
This article presents the first successful application of a capillary electrophoresis-microscale thermophoresis tandem technique (CE-MST) for determining the values of equilibrium constant, realized by connecting online the CE and MST instruments using a fused-silica capillary. The acid-base dissociation of fluorescein isothiocyanate, expressed by the acidity constant value (pKa), was used as a model. The measurement procedure consisted of introducing a mixture containing the analyte and a deliberately added interferent into the CE capillary, electrophoretic separation of the analyte from the interferent, the detection of the analyte with a CE-integrated detector, detection with a MST detector, and then stopping the flow temporarily by turning off the voltage source to conduct the thermophoretic measurement. The analysis of migration times, peak areas and MST responses obtained concurrently for the same sample allowed us to determine the pKa value using three independent methods integrated within one instrumentation. The analyte was effectively separated from the interferent, and the acidity values turned out to be consistent with each other. An attempt was also made to replace the standard commercial CE instrument with a home-made portable CE setup. As a result, the similar pKa value was obtained, at the same time proving the possibility of increasing cost efficiency and reducing energy consumption. Overall, the CE-MST technique has a number of limitations, but its unique analytical capabilities may be beneficial for some applications, especially when sample separation is needed prior to the thermophoretic measurement. 相似文献
23.
The optical properties of two-dimensional (2D) perovskites recently receive numerous research focus thanks to the strong quantum and dielectric confinement effects. In addition to the strong excitonic effect at room temperature, 2D perovskites also have appealing features that their optical properties can be flexibly tuned by alternating organic or inorganic layers. Particularly, 2D chiral perovskites and 2D perovskites based heterostructures are emerging as new platforms to extend their functionalities. To optimize performance of 2D perovskites-based optoelectronic devices, it is critical to understand the fundamentals and explore the strategies to engineer their optical properties. This review begins with an introduction to the excitons and self-trapped excitons of 2D perovskites. Subsequently, inorganic/organic layer effects on optical properties and 2D perovskites based heterostructures are discussed. We also discussed the nonlinear optical properties of 2D perovskite. We are looking forward to that this review can stimulate more efforts to understand and optimize the optical properties of 2D perovskites. 相似文献
24.
Two-dimensional layered materials are considered ideal platforms to study novel small-scale optoelectronic devices due to their unique electronic structures and fantastic physical properties. However, it is urgent to further improve the light–matter interaction in these materials because their light absorption efficiency is limited by the atomically thin thickness. One of the promising approaches is to engineer the plasmonic environment around 2D materials for modulating light–matter interaction in 2D materials. This method greatly benefits from the advances in the development of nanofabrication and out-plane van der Waals interaction of 2D materials. In this paper, we review a series of recent works on 2D materials integrated with plasmonic environments, including the plasmonic-enhanced photoluminescence quantum yield, strong coupling between plasmons and excitons, nonlinear optics in plasmonic nanocavities, manipulation of chiral optical signals in hybrid nanostructures, and the improvement of the performance of optoelectronic devices based on composite systems. 相似文献
25.
J. Hajšlová R. Schoula K. Holadová J. Poustka 《International journal of environmental analytical chemistry》2013,93(2-4):163-173
Abstract The suitability of three GC-ECD systems for the separation of a model mixture containing various congeners of PCBs together with persistent chlorinated aromatics (pesticides and their metabolites, industrial chemicals) was tested. Analyses were performed on two parallel capillaries, the stationary phase of one of them was always in routine practice very common 5% phenyl-methylpolysiloxane, the second one was either 50% phenylmethylpolysiloxane or 7% phenyl-7% cyanopropyl-methylpolysiloxane. The number of unresolved (coeluted) analytes was significantly reduced in these systems and thus unbiased quantitation of PCBs and other components used for regulation was possible. The utilization of this type of multidimensional chromatography for rapid and reliable analysis of real samples (fish oil, human fat) was documented. 相似文献
26.
《Analytical letters》2012,45(15):1127-1132
Abstract Disc electrophoresic can be used to determine if uncookee beef and beef products have Seen adulterated with meat from horses, hogs, deer, and buffalo. 相似文献
27.
《Journal of Coordination Chemistry》2012,65(11):1945-1958
To explore anti-tumor activities of manganese complexes, two complexes have been synthesized and characterized. Complex 1 is bridged by 1,10-phenanthroline and 2,4-biphenyl dicarboxylate. The two complexes have strong fluorescent emission and interact with DNA in an intercalative mode. The complexes also exhibit significant cytotoxic specificity and cancer cell inhibition. 相似文献
28.
应用毛细管区带电泳(CZE)分析测定阿片受体、抗独特型抗羟甲芬太尼抗体等生物大分子样品。熔融石英毛细管柱为60cm×100μmi.d.(从进样到检测器长度为50cm),以硼砂-氢氧化钾为缓冲液。结果表明缓冲液的pH影响CZE对阿片受体的分析,当pH为9.0时分析效果最理想,重现性良好,测得的两个主峰与同批样品进行SDS-PAGE分析得到的两个条带相符。用CZE分析抗独特型抗羟甲芬太尼抗体(IgG),表明用PrOteinA-Sepharose亲和色谱分离得到部分纯化的IgG,迁移时间不同于对照豚鼠的IgG。 相似文献
29.
Structured mirror array for two-dimensional collimation of a chromium beam in atom lithography 总被引:1,自引:0,他引:1 下载免费PDF全文
Direct-write atom lithography,one of the potential nanofabrication techniques,is restricted by some difficulties in producing optical masks for the deposition of complex structures.In order to make further progress,a structured mirror array is developed to transversely collimate the chromium atomic beam in two dimensions.The best collimation is obtained when the laser red detunes by natural line-width of transition 7S3 → 7P40 of the chromium atom.The collimation ratio is 0.45 vertically(in x axis),and it is 0.55 horizontally(in y axis).The theoretical model is also simulated,and success of our structured mirror array is achieved. 相似文献
30.
Stability analysis and control synthesis of uncertain Roesser-type discrete-time two-dimensional systems 下载免费PDF全文
We study the stability analysis and control synthesis of uncertain discrete-time two-dimensional(2D) systems.The mathematical model of the discrete-time 2D system is established upon the well-known Roesser model,and the uncertainty phenomenon,which appears typically in practical environments,is modeled by a convex bounded(polytope type) uncertain domain.The stability analysis and control synthesis of uncertain discrete-time 2D systems are then developed by applying the Lyapunov stability theory.In the processes of stability analysis and control synthesis,the obtained stability/stabilzaition conditions become less conservative by applying some novel relaxed techniques.Moreover,the obtained results are formulated in the form of linear matrix inequalities,which can be easily solved via standard numerical software.Finally,numerical examples are given to demonstrate the effectiveness of the obtained results. 相似文献