Magnolol‐induced inhibition of tumor growth in systemic malignancies

The commentary illustrates the significant tumor attenuating effects of magnolol and refers to the article on “Screening active anti‐breast cancer compounds from Cortex Magnolia officinalis by 2D LC–MS” (X. Hou, et al., J. Sep. Sci. 2013, 36 (4), 706–712). On the basis of the literature, there is definitively need for further studies to fully understand the magnolol reation pathways and to harness the antineoplastic effects.     

傅里叶变换红外光谱法检测脑肿瘤

应用傅里叶变换红外光谱法检测了28例液氮冻存的离体脑肿瘤样本及其残留物(将脑肿瘤样本从ATR的ZnSe晶片上取下,样本在ZnSe晶片上沾染后留下的物质).结果发现,神经鞘瘤和神经上皮组织肿瘤(如星形细胞瘤等)的主要特征吸收峰存在明显差异,因此可从各个特征吸收峰的峰位、峰形及不同谱峰强度比的变化来初步鉴别脑肿瘤的性质,脑肿瘤组织样品残留物的红外光谱也可反映不同性质脑肿瘤的差异.与脂类糖蛋白以及核酸相关的谱带变化分析表明,通过特征峰强比(I1460cm-1/I1400cm-1,I1160cm-1/I1120cm-1和I1160cm-1/I1080cm-1)来鉴别肿瘤的性质与病理诊断结果的符合率超过85%.     

SURFACE MULTI-FUNCTIONALIZATION OF POLY(LACTIC ACID) NANOPARTICLES AND C6 GLIOMA CELL TARGETING in vivo

Polysaccharide coated PLA nanoparticles bearing aldehyde groups were prepared by dialysis of DMSO solution of cholesterol hydrophobic-modified dextran polyaldehyde and PLA against water.The average diameter of the nanoparticles was about 160 nm,and the size distribution was nearly homogenous.The nanoparticles were functionalized simultaneously with CD71 and EGFR antibody through the Schiff's base reaction,and then radiolabeled with ~(99m)Tc.After perfused the radiolabeled nanoparticles into tumor-bearing...     

Quantifying brain tumor tissue abundance in HR‐MAS spectra using non‐negative blind source separation techniques

Given high‐resolution magic angle spinning (HR‐MAS) spectra from several glial tumor subjects, our goal is to differentiate between tumor tissue types by separating the different sources that contribute to the profile of each spectrum. Blind source separation techniques are applied for obtaining characteristic profiles for necrosis, highly cellular tumor and border tumor tissue and providing the contribution (abundance) of each of these tumor tissue types to the profile of each spectrum. The problem is formulated as a non‐negative source separation problem. Non‐negative matrix factorization, convex analysis of non‐negative sources and non‐negative independent component analysis methods are considered. The results are in agreement with the pathology obtained by the histopathological examination that succeeded the HR‐MAS measurements. Furthermore, an analysis to verify to which extent the dimension of the input space, the input features and the number of sources to be extracted from the HR‐MAS data could influence the performance of the source separation is presented. Copyright © 2012 John Wiley & Sons, Ltd.     

Protein-assisted one-pot synthesis and biofunctionalization of spherical gold nanoparticles for selective targeting of cancer cells

All bound up: A small variant of protein?A was used as a biotemplate for the synthesis and biofunctionalization of gold nanoparticles (AuNPs). These functionalized AuNPs were able to bind to antibodies (green blocks, trastuzumab; TZ) with a defined orientation, thus showing promise as bio-nanoparticle systems suitable for selective cell labeling by membrane-receptor-specific recognition.     

肿瘤细胞DNA样品基于二次谐波发生(SHG)的非线性光谱学成像

二次谐波的产生是一个二阶非线性光学过程,这个过程只发生在中心对称系数不为零的非中心对称区域。利用二次谐波显微技术可以对各种具有内源性信号的生物组织进行无损伤实时成像,如结缔组织的胶原纤维或肌细胞的肌动球蛋白。在生物化学和结构生物学中,虽然DNA是由脱氧核糖核苷酸构成而蛋白质是由氨基酸残基组成,但是DNA和蛋白质大分子高级结构的形成机制是相似的。利用光谱学成像技术对不同DNA样品进行检测,获取DNA样品的SHG信号并进行高解析度成像。这些DNA样品包括基因组DNA溶液、细胞核提取物以及培养细胞的细胞核。实验结果表明在常规条件下可以获得基因组DNA溶液和细胞核提取物的SHG信号,但几乎观测不到来自培养细胞核区的SHG信号。通过在培养基中添加少量无水乙醇(体积比小于5%),可以在培养细胞的细胞核区域检测到SHG信号。推测在培养细胞中乙醇和DNA相互作用引起DNA分子构象发生变化,这些变化可能导致了DNA分子非线性光学性质的改变。