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排序方式: 共有735条查询结果,搜索用时 31 毫秒
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Die vorliegende Arbeit beschāftigt sich mit der Answertung von Autoradiogrammen inhomogen markierter mikroskopischer Strukturen am Densitron. Dazu fertigten wir von herkömmlichen Stripping-Filmen (z. B. ORW O K 106) Mikro-Stufengraukeile an und bestimmten die Schwärzung Sijeder Stufe photometrisch. Graukeile geeigneten Schwärzungsumfangs dienten dann zur Einstellung der Farbtrigger des Densitrons, so daß sich die bei dieser Einstellung gemessenen Farbflächen Fi des jeweiligen Objektes bestimmten Schwärzungswerten zuordnen ließen. Als Modellobjekte verwendelen wir erstens Autoradiogramme von 3H-Thymidin-markierten Tumorzellen. Vergleichsmessungen an unterschiedlich lange exponierten, sonst aber identischen Präparaten ergaben eine gute Reproduzierbarkeit der Meßergebnisse. Als zweites Testobjekt dienlen Autoradiogramme einer nur schwach markierten Struktur im Gehirn von Fröschen. Die an diesem Grenzfall erhaltenen Ergebnisse werden mit Meßwerten verglichen, die vom gleichen Objekt mittels Scanning-Photometrie an einem. Mikroskop-Photometer SMP 01 (OPTON, Oberkoches, BRD) gewonnen wurden. 相似文献
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Dr. Zhicheng Yang Dr. Xiaoxiao Fan He Li Xinsheng Li Sifan Li Prof. Zhiyun Zhang Prof. Hui Lin Prof. Jun Qian Prof. Jianli Hua 《Chemistry (Weinheim an der Bergstrasse, Germany)》2021,27(57):14240-14249
Organic small-molecule fluorophores with near-infrared IIa (NIR-IIa) emission have great potential in pre-clinical detection and inoperative imaging due to the high-spatial resolution and deep penetration. However, developments of the NIR-IIa fluorophores are still facing considerable challenges. In this work, a series of diketopyrrolopyrrole (DPP)-based fluorophores were designed and synthesized. Subsequently, nanomaterial T25@F127 with significant NIR-IIa emission properties was rationally prepared by encapsulating DPP-based fluorophore T25 , and was selected for fluorescence angiography and cerebral vascular microscopic imaging with nearly 800 μm penetrating depth and excellent signal-background ratio of 4.07 and 2.26 (at 250 and 400 μm), respectively. Furthermore, the nanomaterial T25@cRGD with tumor targeting ability can image tiny metastatic tumor on intestine with a small size of 0.3 mm×1.0 mm and high-spatial resolution (SBR=3.84). This study demonstrates that the nanomaterials which encapsulated T25 behave as excellent NIR-IIa fluorescence imaging agents and have a great potential for in vivo biological application. 相似文献
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A. S. Bratus’ E. S. Chumerina 《Computational Mathematics and Mathematical Physics》2008,48(6):892-911
A mathematical model of tumor growth therapy is considered. The total amount of a drug is bounded and fixed. The problem is to choose an optimal therapeutic strategy, i.e., to choose an amount of the drug permanently affecting the tumor that minimizes the number of tumor cells by a given time. The problem is solved by the dynamic programming method. Exact and approximate solutions to the corresponding Hamilton-Jacobi-Bellman equation are found. An error estimate is proved. Numerical results are presented. 相似文献
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In this paper, we deal with a nonlinear impulsive differential equations modelling the chemotherapy of a heterogeneous tumor. We consider the case of several drugs with instantaneous effects. We take into account the interactions between sensitive cells and drug resistant cells. We are interested in the stability of the disease. We also study the loss of stability and the bifurcation of nontrivial solutions. 相似文献
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Zahra Mohammadpour Zahra Sadat Hashemi Fatemeh Malekian Jebeli Sahel Ghasemzadeh Esfandyar Askari Mandana Akbary-Yekta Ramin Sarrami-Forooshani 《Particle & Particle Systems Characterization》2021,38(12):2100162
Iron oxychloride (FeOCl) is known for reactive oxygen species (ROS) generation through Fenton chemistry. The activity of FeOCl is preserved in the slightly acidic pH value of the tumor microenvironment (pH 6.5−6.9). Such property can be advantageous in biobased systems, where ROS generation can be modulated in slightly acidic conditions, which is characteristic of the solid tumor microenvironment. In the present study, BSA-stabilized FeOCl nanosheets (NSs) are synthesized and characterized by transmission electron microscope, Fourier transform infrared spectroscopy, zeta potential analysis, dynamic light scattering, and UV–vis spectroscopy. The morphology of the nanoparticles is flake-like, and their hydrodynamic diameter is around 200 nm. MTT, apoptosis assay, and trypan blue staining evaluate the toxicity of FeOCl NSs toward the 4T1 cell line. It is found that the toxicity of the NSs is higher in physiological conditions of solid tumors (pH 6.5, H2O2 100 × 10−6 m ) than in the conditions of healthy organs (pH 7.4). Specifically, cancer cells are in their late apoptotic stage by more than eight times higher at pH 6.5 than pH 7.4. The toxicity results are in agreement with the in vitro catalytic assay of the NSs. Therefore, the FeOCl NSs can be the building blocks for constructing chemodynamic therapy agents. 相似文献
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Kuo-Hsun Chiu Chih-Ching Chang Yu-Chen Chang Jing-Fang Hsu 《Analytica chimica acta》2010,673(2):160-166
Ultrafine carbon black (ufCB) is a potential hazard to the lung. It causes changes in protein expression and it increases alveolar-capillary permeability in the lung. Label-free quantitative proteomic methods allow a sensitive and accurate analytical method for identifying and quantifying proteins in a protein mixture without chemically modifying the proteins. We used a label-free quantitative proteomic approach that combined and aligned LC-MS and LC-MS/MS spectra to analyze mouse bronchoalveolar lavage fluid (BALF) protein changes associated with exposure to ufCB. We developed a simple normalization method for quantification without spiking the internal standard. The intensities of unchanged peptides were used as normalization factors based on a statistical method to avoid the influence of peptides changed because of ufCB. LC-MS/MS spectra and then database searching were used to identify proteins. The relative abundances of the aligned peptides of identified proteins were determined using LC-MS spectra. We identified 132 proteins, of which 77 are reported for the first time. In addition, the expression of 15 inflammatory proteins and surfactant-associated proteins was regulated (i.e., 7 upregulated and 8 downregulated) compared with the controls. Several proteins not previously reported provide complementary information on the proteins present in mouse BALF, and they are potential biomarkers for the understanding of mechanisms involved in ufCB-induced lung disorders hypothesize that using the label-free quantitative proteomic approach introduced here is well suited for more rigorous, large-scale quantitative analysis of biological samples. We hypothesize that this label-free quantitative proteomic approach will be suited for a large-scale quantitative analysis of biological samples. 相似文献
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