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Masami Harimoto Masayuki Yamato Akihiko Kikuchi Teruo Okano 《Macromolecular Symposia》2003,195(1):231-236
We describe a new culture system utilizing the temperature-responsive polymer grafted surface for designing of cell position and layered tissue reconstruction. Organizing of the hepatic tissue structure by controlling the culture system, that is patterned co-culture and layered cell sheet co-culture achieved by moving the cultured cells from the culture surface, resulted in regulation of the hepatocyte function. The technique for cell sheet manipulation would promote the liver tissue engineering in quality. 相似文献