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991.
Costas Giaginis Stamatios Theocharis Anna Tsantili‐Kakoulidou 《Journal of separation science》2013,36(24):3830-3836
The advantageous effect of n‐octanol as a mobile phase additive for lipophilicity assessment of structurally diverse acidic drugs both in the neutral and ionized form was explored. Two RP C18 columns, ABZ+ and Aquasil, were used for the determination of logkw indices, and the results were compared with those previously reported on a base‐deactivated silica column. At pH 2.5, the use of n‐octanol‐saturated buffer as the mobile phase aqueous component led to high‐quality 1:1 correlation between logkw and logP for the ABZ+ column, while inferior statistics were obtained for Aquasil. At physiological pH, the correlations were significantly improved if strongly ionized acidic drugs were treated separately from weakly ionized ones. In the latter case, 1:1 correlations between logD7.4 and logkwoct indices were obtained in the presence of 0.25% n‐octanol. Concerning strongly ionized compounds, adequate correlations were established under the same conditions; however, slopes were significantly lower than unity, while large negative intercepts were obtained. According to the absolute difference (diff = logD7.4–logkw) pattern, base‐deactivated silica showed a better performance than ABZ+, however, the latter seems more efficient for the lipophilicity assessment of highly lipophilic acidic compounds. Aquasil may be the column of choice if logD7.4<3 with the limitation, however, that very hydrophilic compounds cannot be measured. 相似文献
992.
Anders Just Pedersen Petur Weihe Dalsgaard Andrej Jaroslav Rode Brian Schou Rasmussen Irene Breum Müller Sys Stybe Johansen Kristian Linnet 《Journal of separation science》2013,36(13):2081-2089
A broad forensic screening method for 256 analytes in whole blood based on a fully automated SPE robotic extraction and ultra‐high‐performance liquid chromatography (UHPLC) with TOF‐MS with data‐independent acquisition has been developed. The limit of identification was evaluated for all 256 compounds and 95 of these compounds were validated with regard to matrix effects, extraction recovery, and process efficiency. The limit of identification ranged from 0.001 to 0.1 mg/kg, and the process efficiency exceeded 50% for 73 of the 95 analytes. As an example of application, 1335 forensic traffic cases were analyzed with the presented screening method. Of these, 992 cases (74%) were positive for one or more traffic‐relevant drugs above the Danish legal limits. Commonly abused drugs such as amphetamine, cocaine, and frequent types of benzodiazepines were the major findings. Nineteen less frequently encountered drugs were detected e.g. buprenorphine, butylone, cathine, fentanyl, lysergic acid diethylamide, m‐chlorophenylpiperazine, 3,4‐methylenedioxypyrovalerone, mephedrone, 4‐methylamphetamine, p‐fluoroamphetamine, and p‐methoxy‐N‐methylamphetamine. In conclusion, using UHPLC–TOF‐MS screening with data‐independent acquisition resulted in the detection of common drugs of abuse as well as new designer drugs and more rarely occurring drugs. Thus, TOF‐MS screening of blood samples constitutes a practical way for screening traffic cases, with the exception of δ‐9‐tetrahydrocannabinol, which should be handled in a separate method. 相似文献
993.
Ashok Kumar Malik Cristina Blasco Yolanda Picó 《Journal of chromatography. A》2010,1217(25):4018-4040
The use of powerful mass spectrometric detectors in combination with liquid chromatography has played a vital role to solve many problems related to food safety. Since this technique is especially well suited for, but not restricted to the analysis of food contaminants within the food safety area, this review is focused on providing an insight into this field. The basic legislation in different parts of the world is discussed with a focus on the situation within the European Union (EU) and why it favors the use of liquid chromatography–mass spectrometry (LC–MS). Main attention in this review is on the achievements that have been possible because of the latest advances and novelties in mass spectrometry and how these progresses have influenced the best control of food allowing an increase in the food safety and quality standards. Emphasis is given to the potential and pitfalls of the different LC–MS approaches as well as in its possibilities to address current hot issues in food safety, such as the development of large-scale multi-residue methods and the identification of non-target and unknown compounds. Last but not least, future perspectives and potential directions are also outlined highlighting prospects and achievements. 相似文献
994.
Thermal Behaviour of Pharmacologically Active Lithium Compounds 总被引:1,自引:0,他引:1
Tobón-Zapata G. E. Ferrer E. G. Etcheverry S. B. Baran E. J. 《Journal of Thermal Analysis and Calorimetry》2000,61(1):29-35
The thermal decompositions of a series of simple lithium compounds (carbonate, sulfate, acetate, citrates, aspartates and
glutamates) currently used in the treatment of manic-depressive psychosis and related disorders were investigated by means
of TG and DTA measurements in oxygen atmosphere. Pyrolysis residues were characterized by infrared spectroscopy. The stabilities
of the hydrates and intermediate phases generated during the degradation processes are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
995.
《印度化学会志》2021,98(9):100102
Malarial infection due to P. falciparum is prominent cause in worldwide fatality. PfCRT, PfDHPS, PfMDR1 and PfDHFR1 play vital role as targets in malarial infection. We chose PfCRT-specific ligands for our study and tested them against all P. falciparum targets. The study was carried out by performing MDS and MD analyses with the NAMD and AutoDock softwares, respectively. The study's goal is to find potential ligands that can act on all malarial targets at various temperatures.The MDS studies revealed structural conformational changes and protein stability from normal human body temperature, 98.6 °F, to maximum human body temperature, 107 °F. This MDS analysis of Plasmodium targets was carried out by creating a graph of RMSD vs time. Further MD analysis revealed that the ligands reported against PfCRT also had a high binding energy against PfDHPS and PfDHFR. As a result, those ligands can also be targeted for Plasmodium falciparum proteins other than the three mentioned above. These ligands can be subjected to SAR and QSAR studies in order to develop novel molecules for malaria treatment. 相似文献
996.
This work resolves peak compression of charged analytes in CEC with strong cation‐exchange stationary phase particles. By combining electrochromatographic peak shape analysis with the results of numerical simulations and confocal laser scanning microscopy in the packed capillaries, we identify electrical field‐induced concentration polarization as the key physical phenomenon responsible for the inherent existence of local electrical field gradients on the scale of an individual support particle. Consequently, positive and negative field gradients exist between and inside the particles along the whole packing. Their intensity depends on the particles cation‐selectivity (governed by the particles volume charge density and the mobile phase ionic strength) and the applied field strength. The interplay of these local field gradients with the analytes retention (intraparticle adsorption) determines whether fronting, tailing, or spiked analyte peaks are observed, and it provides a mechanism by which strongly retained analytes can be eluted over long distances with little zone dispersion. Our analysis explains the “anomalous” peak compression effects with strong cation‐exchange particles, which have been reported more than a decade ago (Smith, N. W., Evans, M. B., Chromatographia 1995, 41, 197–203) and since then remained largely unresolved. 相似文献
997.
淋巴细胞核DNA—吖啶橙体系荧光抑制法筛选抗癌药物的研究 总被引:6,自引:0,他引:6
以人外周血淋巴细胞为标准细胞,用荧光探针吖啶橙(AO)示踪药物和细胞DNA的作用,提出一种用Hadamard变换显微荧光图象分析法初步筛选抗癌药物的新方法,对五晨特异性抗癌药物(CCNSA)的实验结果和药物细胞DNA作用的原理相符,表明此方法地筛选此类抗癌芗,对四种氯代苯甲醛丙氨酸Schiff碱(CA)金属配合物一淋巴细胞DNA作用,研究结果表明:3-CACo、3-CAZn、2-CAK和4-CAD 相似文献
998.
离子迁移率探测器(Ion Mobility Spectrometry, IMS)在大气环境下, 可以直接对一些物质的分子进行分析, 尤其是可实现对爆炸物品和毒品物质分子的在线检测分析, 显示出它广泛的应用前景. IMS~的电场设计, 是影响IMS物质分辨率的重要因素. 对影响IMS电场的因素进行分析后, 重新设计了IMS结构, 通过对新、旧结构的电场进行模拟、比较、分析, 同时用新设计IMS结构对一些物质进行实际测量分析, 所得结果与国际公认结果对比, 误差小于±4%. 证明新结构电场设计合理有效, 与传统IMS电场设计相比, 是优化的IMS电场结构. 相似文献
999.
1000.