全文获取类型
收费全文 | 68篇 |
免费 | 2篇 |
国内免费 | 13篇 |
专业分类
化学 | 57篇 |
综合类 | 5篇 |
数学 | 2篇 |
物理学 | 19篇 |
出版年
2022年 | 4篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 6篇 |
2012年 | 4篇 |
2011年 | 4篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 3篇 |
2007年 | 5篇 |
2006年 | 4篇 |
2005年 | 2篇 |
2004年 | 5篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
排序方式: 共有83条查询结果,搜索用时 0 毫秒
21.
采用2.71 GHz脉冲微波(脉冲场强为48 kV/m,脉宽为750 ns,脉冲个数分别为200个1次、200个10次、200个20次)辐照SD雄性大鼠和孕鼠,利用流式细胞术、计算机辅助精子分析系统及水迷宫实验分别检测了精子发生过程中生精细胞DNA损伤情况、附睾内精子活力参数变化及子代学习记忆功能。结果表明:2.71 GHz脉冲微波单次辐照会对雄性大鼠的生精过程产生一定的影响,但对附睾内精子质量无明显影响,而多次辐照对精子质量的影响具有累积效应;亲代大鼠在受照后,其子代的生长在短期会受阻,且学习、记忆能力也会受到一定的影响。 相似文献
22.
余多慰 《光谱学与光谱分析》1998,18(3):303-306
利用拉曼散射光谱测试技术,对不同温度处理后获得的各种相对湿度下的鲱精DNA纤维进行脱氧核苷C—H振动与结合水O—H振动行为分析,以揭示其中结合水的数量特征。结果表明,运用25926×I2964/I3024新指标所获数据与文献报道的小牛胸腺DNA水合状态拉曼数据不同,但与小牛胸腺DNA、鲑精DNA的红外、X射线衍射、重量分析及差示扫描量热分析所得数据相吻合,证明拉曼分析公式的修订是成功的。鲱精DNA纤维应与上述二种DNA纤维具有同样的水合状态,每核苷酸对含有9~14个结合水分子。 相似文献
23.
乙酸对DNA空间结构微观损伤的Raman光谱特征 总被引:1,自引:0,他引:1
利用Raman光谱技术从分子水平研究了乙酸对鲱鱼精DNA空间结构微观损伤的特征。实验结果表明,鲱鱼精DNA被乙酸浸润处理后,Raman特征频率与强度均发生了不同程度的变化。证明了乙酸对DNA的损伤是整个分子,包括骨架磷酸基团、脱氧核糖及碱基。不仅是DNA的构象,而且其构型也发生了变化:氢键断裂,B型构象被修改,DNA单、双链断裂,碱基也受到了严重损伤。其碱基损伤严重程度依次为胞嘧啶、胸腺嘧啶、鸟嘌呤和腺膘呤。 相似文献
24.
25.
A method for quantitative determination of fish sperm deoxyribonucleic acid(fsDNA) was developed by using titanium dioxide(TiO2) as an adsorbent and near-infrared diffuse reflectance spectroscopy(NIRDRS). The selective enrichment of fsDNA was proved by comparing the adsorption efficiency of bovine serum albumin, tyrosine and tryptophan, and the low adsorption background of TiO2 was illustrated by comparing the spectra of four commonly-used inorganic adsorbents(alkaline aluminium oxide, neutral aluminium oxide, nano-hydroxyapatite and silica). The spectral feature of fsDNA can be clearly observed in the spectrum of the sample. Partial least squares(PLS) model was built for quantitative determination of fsDNA using 28 solutions, and 13 solutions with interferences were used for validation of the model. The results showed that the correlation coefficient(R) between the predicted and the reference concentration is 0.9727 and the recoveries of the validation samples are in the range of98.2%–100.7% 相似文献
26.
鲱精DNA纤维的差示扫描量热(DSC)谱中,除存在一个高温区的变性峰以外,还有3个低温区的吸热峰,类似于蛋白质DSC分析中出现的“变性前峰”。经过25315K(-20℃)冻结处理的DNA纤维,空间结构稳定性提高,变性前峰峰温增加4~8K。冻结与非冻结两类样品中,变性前峰的焓值分别为-10253和-7765kJ·mol-1。作者探讨了DNA变性前峰的成因,并认为DNA变性过程中的多元性可能与碱基序列特异性有关。 相似文献
27.
建立了多壁碳纳米管修饰玻碳电极(MWNTs/GCE)测定甲巯咪唑(TMZ)的电化学分析新方法,研究了TMZ与DNA的相互作用,探讨了TMZ与DNA相互作用的机理。实验发现TMZ在pH=7.0的磷酸盐缓冲溶液中于0.29V处有一氧化峰,其峰电流与TMZ的浓度在3.0~100μmol/L范围内呈良好的线性关系,检出限(S/N=3)为1.0μmol/L。对30.0μmol/L的TMZ进行11次平行测定,其相对标准偏差(RSD)为3.3%。当不同浓度的鲱鱼精DNA加入TMZ溶液后,其氧化峰电流降低,表明两者发生了插入作用,形成了非电活性化合物。紫外光谱红移增色效应说明TMZ嵌插入DNA双链之间,二者结合比为1∶2,结合常数为9.93×106 L/mol。 相似文献
28.
Non-ionic surfactant vesicles (NSVs), also referred to as niosomes, have been studied as an alternative to conventional liposomes. In this paper, electrochemical inspection of the interaction between Herring sperm DNA and niosomes has been investigated after a simple and novel method for the formation of niosomes on Au electrode. Each step of electrode modification has been confirmed with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The assembly of octadecanethiol (ODT) layer on the electrode surface generates a packed film that introduces a barrier to the interfacial electron transfer (R(et)), and the subsequent immobilization of niosomes onto the self-assembled monolayer (SAM) layer results in a further increase of R(et), due to the formed bilayer almost blocked the redox probe to the electrode surface. When Herring sperm DNA was added, the R(et) value decreased, indicating that the barrier of the redox probe to the surface was disrupted. The addition of DNA caused the formation of some transmembrane channels for the redox probe across the niosomes. A good linear relationship between R(et) value and DNA concentration was found over the 0-0.05 mg mL(-1) concentration range. 相似文献
29.
30.
AbstractAmphibian egg-jelly coat plays an important role in successful fertilization and development. Here, we ask whether proteins like allurin in the jelly coats of frog eggs might influence fertilization rate success. Using in vitro fertilization of Albanian water frog, Pelophylax shqipericus, we found that body cavity eggs or eggs deprived of jelly coat were not fertilized, compromising the success of in vitro fertilization procedure. When de-jellied eggs were inseminated with sperm suspension, the fertilization efficiency is dramatically decreased even inhibited, suggesting that the gel structure is one of the major factors in the achievement of fertilization in the frogs. Fertilization of de-jellied eggs with sperm pre-treated with egg jelly coat, restored the fertilization competency. Such a result suggests that egg jelly coat probably guides the sperm to the egg surface while maintaining the fertilization ability, contributing to a successful in vitro fertilization of Pelophylax shqipericus. 相似文献