Preparation and properties of polymer and quantum dot composites

Quantum dots (QDs) were prepared in an organic system through a simple and low-cost wet chemistry method. Polymer beads with a diameter of 60–70 nm and specific functional groups were synthesized by a particular seeded emulsion polymerization technique. QDs were embedded in the polymer beads with the specific functional groups through dissolving and swelling method, which provided the condition for the conjunction of biomolecules and QDs as fluorescent probes. The prepared composites were characterized with UV-Vis, PL, TEM, FTIR, CLSM and conductance titration etc. The results show that QDs are successfully embedded in polymer beads, which breaks the limitation that the conjunction of biomolecules and QDs can be achieved only for those synthesized in aqueous system. __________ Translated from Journal of Shanghai Jiaotong University, 2005, 39(1) (in Chinese)     

光学薄膜氧气传感器研究进展

光学薄膜氧气传感器具有检测精度高、选择性好、抗干扰能力强等优点,近年来日益得到人们的广泛重视,研究工作也在不断深化和拓展,展现出十分广阔的应用前景。本文按光学薄膜氧气传感器的制备方法分类扼要综述了光学薄膜氧气传感器的研究现状,并展望了其研究前景。     

用同步荧光法研究细胞色素C在水溶液中的存在形态

用同步荧光光谱法研究细胞色素Ｃ在水溶液中的存在形态，发现细胞色素Ｃ中色氨酸残基的同步荧光光谱随浓度改变而发生变化，在较稀的溶液中，只观察到色氨酸残基的一个荧光峰，在较浓的溶液中也观察到一个荧光峰，而在这两个浓度之间时，这两个峰共存，可能反映了细胞色素Ｃ在不同浓度溶液中聚集状态的不同，当向存在两个荧光峰的溶液中加入不同浓度的脲，在一定脲浓度范围内，细胞色素Ｃ不发生变性，脲的作用使细胞色素Ｃ的单体浓度     

原子吸收、原子荧光和火焰光谱分析

本文主要根据1991～1993年第一季度国内重要分析刊物所发表的文章以及在此期间重要全国性会议的主要论文,对我国原子吸收光谱法,原子荧光光谱法以及火焰发射光谱分析法的进展作一综述。文中共收集文献692篇。     

Hole Burning and Fluorescence Spectra of Sol-Gel Derived Sm2+-Doped Glasses

Sm2+-doped glasses in the system of Al2O3-SiO2 were prepared by sol-gel processing of metal alkoxides and reacting with H2 gas at 800°C. The hole burning properties of these glasses were investigated. The holes were burned in the 7F0 5D0 line of the Sm2+ ions using a DCM dye laser at 77 K. The hole depth increased with increasing the laser irradiation time, reaching up to 15% of the total intensity within a few hundred seconds. The hole width was 3 cm–1 full width at half maximum. Fluorescence line narrowing spectra of the 5D0 7 F1 transition were analyzed to study the local structure surrounding the Sm2+ ion. It is concluded that the Sm2+ ions are closely coordinated with nine oxygens of the AlO6 group in aluminosilicate glasses and that the addition of Al3+ ions into glass induces an increase in the coordination number of the Sm2+.     

微波消解-氢化物发生原子荧光法测定塑料原料及其制品中的砷、汞

采用微波消解进行样品前处理，以硫脲为预还原剂，用氢化物发生原子荧光法测定塑料原料及其制品中的砷、汞。测定砷和汞的线性范围均为0～8μg／L，砷、汞的检出限分别为0．005、0．076μg／L，测定结果的相对标准偏差分别为4.96％～7．38％、2．94％～7．20％(n=6)，回收率分别为92．0％～103．2％、92．0％～98.0％。     

铽与苯乙酮酸、2,2''''-联吡啶、1,10-菲口罗啉、三苯基氧膦铽三元配合物的合成、结构表征及荧光性能的研究

以硝酸铽、苯乙酮酸（HL）, 2, 2＇-联吡啶（dipy）、 1, 10-菲啰啉（phen）和三苯基氧膦（TPPO）合成了TbL3dipy（H2O）2, TbL3phen（H2O）2和TbL2（TPPO）2NO3 3种新型固态配合物. 用元素分析、电导率、红外光谱和核磁共振谱对其进行了表征, 确定了配合物的组成. IR表明, 配合物中羧酸根可能以单齿方式配位. 1HNMR显示, 苯乙酮酸根配位后苯环上5个氢原子的化学位移移向高场. 室温下测定了配合物的荧光激发光谱, TbL3dipy（H2O）2, TbL3phen（H2O）2和TbL2（TPPO）2NO3最佳激发波长分别为361.0, 359.0和367.0 nm;分别以最佳激发波长测定了配合物的发射光谱, 配合物TbL3dipy（H2O）2, TbL3phen（H2O）2 和TbL2（TPPO）2NO3显示Tb3＋离子的特征发射光谱, TbL3dipy（H2O）2和TbL3phen（H2O）2均产生四条谱带, 分别归属于^5D4-^7Fj（j= 6, 5,4,3）能级跃迁（TbL3dipy（H2O）2： 489.0, 545.0, 584.0, 620.0 nm;TbL3phen（H2O）2： 490.0, 544.0, 583.0, 620.0 nm）;但在相同测定条件下, TbL2（TPPO）2NO3仅显示5D4-7F5（544.0 nm）能级跃迁光谱. 3种配合物中TbL3dipy（H2O）2发光强度最高.     

Application of capillary electrophoretic immunoassay to analysis of estradiol in women's serum

Summary A rapid and sensitive capillary electrophoretic immunoassay is described for determination of estradiol in women's serum. Addition of thermally reversible hydrogel in the buffer, serving as a replaceable packing material, improved the reproducibility of the method. Using a laser-induced fluorescence detector this method can be applied to the determination of estradiol at concentrations as low as 30.6 pg mL¹. Estradiol levels in 16 normal women's serum were measured at the range 115≈370 pg mL¹. The results of this method have been found to correlate well with those of chemiluminescent immunoassay.     

水杨酸与烟草铜锌超氧化物歧化酶的相互作用

利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸（SA）对超氧化物歧化酶（SOD）活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制，求得两者结合的形成常数和配位数，使用Forster非辐射能量转移技术求出了两者的结合位置距色氨酸残基间的距离为2.60nm。     

流动注射在线共沉淀HG-AFS测定痕量铅

建立了流动注射在线共沉淀HG-AFS测定痕量铅的分析方法。方法基于铅在碱性介质中与氢氧化镁共沉淀，沉淀收集在锥形沉淀腔中，用HCl溶洗沉淀和NaBH4反应，产生的氢化物被载气引入原子化器中进行测定。测定铅的RSD为3％(5ng/mL，n=10)，检出限为0．01ng。检出限较直接进样降低了11倍。对国家标准物质中铅的测定结果与标准值相符。