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101.
L. Ouali S. Stoll E. Pefferkorn A. Elaissari V. Lanet C. Pichot B. Mandrand 《先进技术聚合物》1995,6(7):541-546
The paper describes a study of the kinetics and mechanism of the coagulation of two types of immunoassays using sensitized latexes. The positive response to the first test is based on the aggregation of the gamma globulin (IgG)-coated polystyrene latexes in the presence of IgM rheumatoid antigen. The second test is relative to the heteroaggregation of two types of sensitized latexes induced by the presence of human chorionic gonadotropin (HCG). In the latter test, two identical polystyrene latexes bearing carboxylic acid surface groups were sensitized by covalent coupling of monoclonal antibodies specific for the αHCG determinant on one type of latex and for the βHCG determinant on the other type. Using the Coulter Counter method, the aggregate size distribution c(n) was determined as a function of the number n of elementary constituents, thus enabling calculation of the number N(t) and weight S(t) average sizes of the aggregates. The temporal variations of the average sizes were compared with typical situtions of reaction-limited aggregation processes in order to characterize the mechanism of aggregation induced by antibody–antigen reactions. 相似文献
102.
103.
Yan Feng ZHANG Zhi Xian GAO Qing Min ZHANG Shu Gui DAI 《中国化学快报》2006,17(8):1021-1024
Aldicarb, 2-methyl-2-(methylthio)propionaldehyde O-(methylcarbamoyl)oxime, is a systemic N-methylcarbamate insecticide and has been used on a variety of crops. It is moderately water soluble and considered as one of the most acutely toxic pesiticides. Ald… 相似文献
104.
Thermal Buckling Analysis of FGM Sandwich Circular Plates Under Transverse Nonuniform Temperature Field Actions北大核心CSCD
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Based on the von Kármán geometric nonlinear plate theory, the displacement⁃type geometric nonlinear governing equations for FGM sandwich circular plates under transverse nonlinear temperature field actions were derived. With the immovable clamped boundary condition, the analytical formula for dimensional critical buckling temperature differences of the system was obtained from the solution of the linear eigenvalue problem. Moreover, the 2⁃point boundary value problem of ordinary differential equations was solved with the shooting method. The effects of geometric parameters, constituent material properties, gradient indexes, temperature field parameters and layer⁃thickness ratios on the critical buckling temperature differences, the thermal postbuckling equilibrium paths, and the buckling equilibrium configurations of FGM sandwich circular plates, were investigated. The results show that, with the increases of the thickness⁃radius ratio, the relative thickness of the FGM layer and the gradient index, the FGM sandwich circular plate's critical buckling temperature difference will increase monotonically. Given a fixed radius and a fixed total thickness, the postbuckling deformation of the FGM sandwich circular plate will decrease significantly with the relative thickness of the FGM layer. © 2023 Editorial Office of Applied Mathematics and Mechanics. All rights reserved. 相似文献
105.
Yu. V. Kokhanenko 《International Applied Mechanics》2006,42(9):1045-1051
A thin rectangular sandwich plate with isotropic linear elastic layers is considered. The plate is in a plane-strain state
under uniaxial compression. An exact statement of the buckling problem is given. Its approximate solution is found by the
finite-difference method. The concept of base scheme is used to formulate discrete problems in explicit and compact form.
As an example, the critical parameters of the plate are calculated using a computation optimization procedure. Its efficiency
is demonstrated
__________
Translated from Prikladnaya Mekhanika, Vol. 42, No. 9, pp. 98–105, September 2006. 相似文献
106.
107.
The dynamic thermomechanical problem for thin-walled laminated elements is formulated based on the geometrically linear theory and Kirchhoff–Love hypotheses. A simplified model of vibrations and dissipative heating of structurally inhomogeneous inelastic bodies under harmonic loading is used. The mechanical properties of materials are described using strain-dependent complex moduli. A nonstationary vibration-heating problem is solved. The dissipative function, derived from the stationary solution, is used to specify internal heat sources. The amplitude–frequency characteristics and spatial distributions of the main field variables are studied for a sandwich beam subjected to forced vibrations 相似文献
108.
Minghui Chen Zhibiao Yu Daofeng Liu Tao Peng Kun Liu Shuying Wang Yonghua Xiong Hua Wei Hengyi Xu Weihua Lai 《Analytica chimica acta》2015
Two patterns of signal amplification lateral flow immunoassay (LFIA), which used anti-mouse secondary antibody-linked gold nanoparticle (AuNP) for dual AuNP-LFIA were developed. Escherichia coli O157:H7 was selected as the model analyte. In the signal amplification direct LFIA method, anti-mouse secondary antibody-linked AuNP (anti-mouse-Ab-AuNP) was mixed with sample solution in an ELISA well, after which it was added to LFIA, which already contained anti-E. coli O157:H7 monoclonal antibody-AuNP (anti-E. coli O157:H7-mAb-AuNP) dispersed in the conjugate pad. Polyclonal antibody was the test line, and anti-mouse secondary antibody was the control line in nitrocellulose (NC) membrane. In the signal amplification indirect LFIA method, anti-mouse-Ab-AuNP was mixed with sample solution and anti-E. coli O157:H7-mAb-AuNP complex in ELISA well, creating a dual AuNP complex. This complex was added to LFIA, which had a polyclonal antibody as the test line and secondary antibody as the control line in NC membrane. The detection sensitivity of both LFIAs improved 100-fold and reached 1.14 × 103 CFU mL−1. The 28 nm and 45 nm AuNPs were demonstrated to be the optimal dual AuNP pairs. Signal amplification LFIA was perfectly applied to the detection of milk samples with E. coli O157:H7 via naked eye observation. 相似文献
109.
We reported here for the first time on the use of cotton thread combined with novel gold nanoparticle trimer reporter probe for low-cost, sensitive and rapid detection of a lung cancer related biomarker, human ferritin. A model system comprising ferritin as an analyte and a pair of monoclonal antibodies was used to demonstrate the proof-of-concept on the dry-reagent natural cotton thread immunoassay device. Results indicated that the using of novel gold nanoparticle trimer reporter probe greatly improved the sensitivity comparing with traditional gold nanoparticle reporter probe on the cotton thread immunoassay device. The assay avoids multiple incubation and washing steps performed in most conventional protein analyses. Although qualitative tests are realized by observing the color change of the test zone, quantitative data are obtained by recording the optical responses of the test zone with a commercial scanner and corresponding analysis software. Under optimal conditions, the cotton thread immunoassay device was capable of measuring 10 ng/mL human ferritin under room temperature which is sensitive enough for clinical diagnosis. Moreover, the sample solution employed in the assays is just 8 μL, which is much less than traditional lateral flow strip based biosensors. 相似文献
110.
Rong-Liang Liang Xu-Ping Xu Tian-Cai Liu Jian-Wei Zhou Xian-Guo Wang Zhi-Qi Ren Fen Hao Ying-Song Wu 《Analytica chimica acta》2015
Alpha-fetoprotein (AFP), a primary marker for many diseases including various cancers, is important in clinical tumor diagnosis and antenatal screening. Most immunoassays provide high sensitivity and accuracy for determining AFP, but they are expensive, often complex, time-consuming procedures. A simple and rapid point-of-care system that integrates Eu (III) chelate microparticles with lateral flow immunoassay (LFIA) has been developed to determine AFP in serum with an assay time of 15 min. The approach is based on a sandwich immunoassay performed on lateral flow test strips. A fluorescence strip reader was used to measure the fluorescence peak heights of the test line (HT) and the control line (HC); the HT/HC ratio was used for quantitation. The Eu (III) chelate microparticles-based LFIA assay exhibited a wide linear range (1.0–1000 IU mL−1) for AFP with a low limit of detection (0.1 IU mL−1) based on 5ul of serum. Satisfactory specificity and accuracy were demonstrated and the intra- and inter-assay coefficients of variation (CV) for AFP were both <10%. Furthermore, in the analysis of human serum samples, excellent correlation (n = 284, r = 0.9860, p < 0.0001) was obtained between the proposed method and a commercially available CLIA kit. Results indicated that the Eu (III) chelate microparticles-based LFIA system provided a rapid, sensitive and reliable method for determining AFP in serum, indicating that it would be suitable for development in point-of-care testing. 相似文献