Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment

This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently.     

汽车碰撞砼护拦过程的数值模拟

将汽车与砼护拦简化为刚体，给出了描述碰撞过程中车辆三维空间运动的Ｅｕｌｅｒ－Ｌａｇｒａｎｇｅ方程－微分代数方程组，采用半显式外插方法求解上述方程组。数值计算得出的车辆各方向上的最大平均加速度、最大侧倾角、最大俯仰角、车轮沿护拦面的最大爬升值及车辆脱离护拦时的弹出角等结果是从乘员风险和车辆轨迹等多角全面评估砼护拦的安全性能的重要依据。针对美国ＮｅｗＪｅｒｃｙ型砼护拦的实际算例与文献结果吻合较好。     

Quantitative assessment of moisture damage for cacao bean quality using two-dimensional gas chromatography combined with time-of-flight mass spectrometry and chemometrics

Quality control of cacao beans is a significant issue in the chocolate industry. In this report, we describe how moisture damage to cacao beans alters the volatile chemical signature of the beans in a way that can be tracked quantitatively over time. The chemical signature of the beans is monitored via sampling the headspace of the vapor above a given bean sample. Headspace vapor sampled with solid-phase micro-extraction (SPME) was detected and analyzed with comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC × GC–TOFMS). Cacao beans from six geographical origins (Costa Rica, Ghana, Ivory Coast, Venezuela, Ecuador, and Panama) were analyzed. Twenty-nine analytes that change in concentration levels via the time-dependent moisture damage process were measured using chemometric software. Biomarker analytes that were independent of geographical origin were found. Furthermore, prediction algorithms were used to demonstrate that moisture damage could be verified before there were visible signs of mold by analyzing subsets of the 29 analytes. Thus, a quantitative approach to quality screening related to the identification of moisture damage in the absence of visible mold is presented.     

A cost-effective WDM-PON architecture simultaneously supporting wired, wireless and optical VPN services

It is believed that next-generation passive optical networks (PONs) are required to provide flexible and various services to users in a cost-effective way. To address this issue, for the first time, this paper proposes and demonstrates a novel wavelength-division-multiplexed PON (WDM-PON) architecture to simultaneously support three types of services: 1) wireless access traffic, 2) optical virtual passive network (VPN) communications, and 3) conventional wired services. In the optical line terminal (OLT), we use two cascaded Mach-Zehnder modulators (MZMs) on each wavelength channel to generate an optical carrier, and produce the wireless and the downstream traffic using the orthogonal modulation technique. In each optical network unit (ONU), the obtained optical carrier is modulated by a single MZM to provide the VPN and upstream communications. Consequently, the light sources in the ONUs are saved and the system cost is reduced. The feasibility of our proposal is experimentally and numerically verified.     

Remarks on Higgs inflation

We discuss models where the Higgs boson of the electroweak standard model plays the role of the inflaton. We focus on the question of the violation of perturbative unitarity due to the coupling of the Higgs boson either to the Ricci scalar or to the Einstein tensor and discuss the background dependence of the unitarity bounds. Our conclusion is that the simplest model which restricts itself to the standard model Higgs boson without introducing further degrees of freedom has a serious problem. However, in the asymptotically safe gravity scenario, the Higgs boson of the standard model could be the inflaton and no physics beyond the standard model is required to explain both inflation and the spontaneous breaking of the electroweak symmetry of the standard model.     

从“福岛第一核电站事故”看我国核能利用的核安全

文章简要说明了福岛第一核电站事故的起因、发展和后果,并对核电站全厂断电、反应堆压力容器及安全壳超压、氢爆等导致事故恶化的原因作了分析.文章还就导致福岛核事故的相关因素与我国核电站的安全设计及防范对策进行了分析比较.在吸取福岛核事故的经验教训方面,文章提出了若干值得引发关注的启示,作为我国在建和在役核电站的改进,以及新设计核电站的参考.文章还就我国能源的状况,核能发展的必要性,以及核能发展的方针进行了探讨.     

有超车规则的交通流的元胞自动机模型

分析和评论了2014年美国大学生数学建模竞赛（MCM）A题,以及获得outstanding winner奖的6篇论文。首先对试题进行分析,并结合已有文献指出A题的主要解题思路;然后,通过评述获奖论文,指出学生论文的优点及存在的问题;最后,对今年赛题的某些问题进行讨论。     

基于非适应性组合群测的多路存取信道模型的构作

在多路存取信道中当前用户的信息传输是计算机通信网络的一个重要内容,基于组合群测理论构作了多路存取信道模型,介绍了它在多路存取信道中当前用户信息传输方面的应用.     

基因武器威胁及杀伤模型浅析

随着基因嫁接技术的日益成熟,人类可能面临新一轮全球基因武器竞赛.科学家们就发出警告说,能够使一个种族从地球上消失的基因武器有可能在5年内变成现实,原因是阻止生物物科技武器发展"重中之重"——基因武器发展的"机会窗口"正在缩小,因此对基因武器的发展进行研究具有一定的理论和实践意义.论述了基因武器发展对我国安全的威胁以及难以控制的理由,建立了基因武器杀伤模型.     

Effects of rainfall infiltration on deep slope failure

With the finite element method and the limit equilibrium method, a numerical model has been established for examining the effects of rainfall infiltration on the stability of slopes. This model is able to availably reflect the variations in pore pressure field in slopes, dead weight of soil, and the softening of soil strength caused by rainfall infiltration. As a case study, an actual landslide located at the Nongji Jixiao in Chongqing is studied to analyze the effects of rainfall infiltration on the seepage field and the slope stability. The simulated results show that a deep slope failure is prone to occur when rainfall infiltration will lead to a remarkable variation in the seepage field, in particular, for large range pore water pressure increase in slopes. Supported by the Knowledge Innovation Project of Chinese Academy of Sciences (Grant No. KJCX2-SW-L1-4) and the Major State Basic Research Development Program of China (Grant No. 2002CB412703)