A strategy to sequence repetitive DNA based on partial restriction enzyme cleavage

The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers.     

Finiteness of the strong global dimension of radical square zero algebras

The strong global dimension of a finite dimensional algebra A is the maximum of the width of indecomposable bounded differential complexes of finite dimensional projective A-modules. We prove that the strong global dimension of a finite dimensional radical square zero algebra A over an algebraically closed field is finite if and only if A is piecewise hereditary. Moreover, we discuss results concerning the finiteness of the strong global dimension of algebras and the related problem on the density of the push-down functors associated to the canonical Galois coverings of the trivial extensions of algebras by their repetitive algebras.     

WHOLE-CELL CLAMP STUDY OF XENOPUS EMBRYONIC CHOLINERGIC NEURONS

Whole-cell clamped myoballs are placed into direct visible contact with the growth cones of isolated neurons in embryonic Xenopus culture to serve as probe of acetylchollne (AcCHo) release in order to determine whether these neurons are cholinergic or not. Using a GQ-seal, whole-cell recording method, the electrophysiological properties of these identified cholinergic neurons are studied. It is found that these embryonic neurons, like adult frog motor neurons, exhibit repetitive firings in a certain embryonic developing stage. A development of repetitive firings is observed simultaneously. Tracing the development of one neuron, we find that the development of repetitive firing is completed at the 48th h after fertilization. Tetrodotoxin (TTX) which blocks Na~+ channels can abolish all firings; and tetraethyl ammonium chloride (TEA), the blocker of K~+ channels, reverses this development, i. e. it makes the repetitive firings disappear again. These data show that the nature of the development of repetiti     

重复频率纳秒脉冲聚四氟乙烯薄膜击穿特性

实验研究了聚四氟乙烯薄膜在重复频率纳秒脉冲下的击穿特性,选用脉冲上升时间约15 ns,脉宽30~40 ns,重复频率1~1 000 Hz。测量并计算了击穿前后的电压电流波形、重复频率耐受时间和施加脉冲个数与击穿特性密切相关的参数。结果表明,重复频率纳秒脉冲下聚四氟乙烯薄膜击穿场强为MV/cm量级,重复频率耐受时间随施加场强和重复频率的增大而减小。薄膜本身性质及油浸时间使实验数据具有分散性,重复频率纳秒脉冲下聚四氟乙烯薄膜击穿应考虑重复频率条件下的热积累效应和材料缺陷。     

1.2MV重复频率脉冲电源的测试诊断系统

为了更好分析系统的放电特性和工作状态,优化脉冲功率源系统的设计,研制了一套1.2MV重复频率高功率微波驱动电源测试诊断系统。通过研究高电压、大电流测试技术和数据采集、信号处理、故障诊断技术,同时运用虚拟仪器和电磁兼容等技术,探索开发了一套重复频率脉冲电压电流测试诊断系统。结合系统的研制需求,通过分析各种数据采集设备的优缺点,硬件选用了凌华公司的PXI数据采集系统,软件采用LabVIEW,设计开发了重复频率快信号的采集诊断程序,研制出实时诊断系统,并在1.2MV重复频率脉冲电源系统中应用,实现了对重复频率电源关键点电压电流的测试以及数据的诊断、处理和远程在线监测,出现故障时报警、存储数据并自动断电等功能。     

非脆弱离散重复控制的二维模型方法

针对一类线性离散系统,提出一种基于二维模型的非脆弱离散重复控制设计方法.通过独立地考虑重复控制系统的控制与学习行为,建立离散重复控制系统的二维模型. 在此基础上,针对重复控制器和反馈控制器具有不确定性的离散重复控制系统,给出了基于线性矩阵不等式的系统稳定性条件和重复控制律. 最后,数值仿真实例验证了所提方法的有效性.     

低抖动快前沿重复频率高压脉冲触发源研制

给出了基于远程和本地两种控制方式,作为低抖动快前沿重复频率高压脉冲触发源系统的设计原理和方法。研制了一台重复频率为0.01~1Hz、脉冲输出幅度为10~20kV、前沿小于10ns、脉宽大于500ns以及抖动小于1ns的高压脉冲触发源。设计上将程控和手动触发信号分别作为重复频率和单次预触发脉冲,驱动后级触发器绝缘栅双极型晶体管,经脉冲变压器变换后控制氢闸流管VE4141放电,实现输出高压脉冲。通过实验结果验证了所采用的设计原理及方法的可行性,给出了外触发脉冲情况下高压脉冲输出的实验结果。     

Wakamatsu-silting complexes

We introduce Wakamatsu-silting complexes (resp., Wakamatsu-tilting complexes) as a common generalization of both silting complexes (resp., tilting complexes) and Wakamatsu-tilting modules. Characterizations of Wakamatsu-silting complexes are given. In particular, we show that a complex T is Wakamatsu-silting if and only if its dual DT is Wakamatsu-silting. It is conjectured that compact Wakamatsu-silting complexes are just silting complexes. We prove that the conjecture lies under the finitistic dimension conjecture.     

全柔性空间机器人运动振动一体化输入受限重复学习控制

探究基座、臂、关节全柔性影响下空间机器人动力学模拟、运动控制及基座、臂、关节三重柔性振动主动抑制的问题,设计了不基于系统模型信息的运动振动一体化输入受限重复学习控制算法.将柔性基座与关节等效为线性弹簧与扭转弹簧,柔性臂视为欧拉-伯努利梁模型,利用拉格朗日方程与假设模态法建立动力学模型,然后,用奇异摄动理论将模型分解为包含刚性变量与臂柔性振动的慢变子系统,包含基座、关节柔性振动的快变子系统,并分别设计相应的子控制器,构成了带关节柔性补偿的一体化控制算法.针对慢变子系统,提出输入受限重复学习控制算法,由双曲正切函数,饱和函数与重复学习项构成,双曲正切函数与饱和函数实现输入力矩受限要求,重复学习项补偿周期性系统误差,以完成对基座姿态、关节铰周期轨迹的渐进稳定追踪.然而,为了同时抑制慢变子系统臂的柔性振动,运用虚拟力的概念,构造同时反映臂柔性振动与系统刚性运动的混合轨迹,提出了基于虚拟力概念的输入受限重复学习控制器,保证基座、关节轨迹精确追踪的同时,对臂的柔性振动主动抑制.针对快变子系统,采用线性二次最优控制算法抑制基座与关节的柔性振动.仿真结果表明:控制器适用于一般柔性非线性系统,满足输入力...     

一种重复频率脉冲导引磁场的设计

介绍了一种重复频率脉冲磁场作为高功率微波源的导引磁场的设计,该磁场电源具有功耗低、发热量小、结构紧凑等优点,符合高功率微波朝重复频率方向发展的需求。从产生脉冲磁场的电流表达式出发,根据涡流损耗不能太大、品质因数要高和电容储能要小的原则,给出了脉冲磁场产生系统的储能电容和充电电压的优化设计方法。最后将此方法应用于Ka波段返波振荡器导引磁场的设计,确定出产生脉冲磁场电路的电容和充电电压的值,并进行了仿真和实验研究,结果与理论要求吻合较好,在重复频率10 Hz条件下能稳定运行,验证了设计的合理性。