Silk fibroin is a useful protein polymer for biomaterials and tissue engineering. In this work, porogen leached scaffolds prepared from aqueous and HFIP silk solutions were reinforced through the addition of silk particles. This led to about 40 times increase in the specific compressive modulus and the yield strength of HFIP‐based scaffolds. This increase in mechanical properties resulted from the high interfacial cohesion between the silk matrix and the reinforcing silk particles, due to partial solubility of the silk particles in HFIP. The porosity of scaffolds was reduced from ≈90% (control) to ≈75% for the HFIP systems containing 200% particle reinforcement, while maintaining pore interconnectivity. The presence of the particles slowed the enzymatic degradation of silk scaffolds.
An efficient and highly versatile synthesis of two libraries 1(x,y) and 2-Ar(x,y,z) or 2-R2(x,y,w) based on the privileged benzimidazole scaffold is described. Our design is aimed at obtaining molecules, biased for binding to RNA targets, by incorporating functionalities, which are frequently found in natural RNA-ligands. The library construction was realized with the use of SPOS in high average yields and purity. Monitoring and quantitation of intermediates and final products were performed by the use of NMR spectroscopy using DMFu as an internal standard. 相似文献
The naturally occurring neocryptolepine (5-Methylindolo [2,3-b]quinoline) and its analogs exhibited prominent anticancer and antimalarial activity. However, the main problem of this class of compounds is their poor aqueous solubility, hampering their bioavailability and preventing their clinical development. To overcome the problem of insolubility and to improve the physicochemical and the pharmacological properties of 5-Methylindolo [2,3-b]quinoline compounds, this work was designed to encapsulate such efficient medical compounds into mesoporous silica oxide nanoemulsion (SiO2NPs). Thus, in this study, SiO2NPs was loaded with three different concentrations (0.2 g, 0.3, and 0.6 g) of 7b (denoted as NPA). The findings illustrated that the nanoparticles were formed with a spherical shape and exhibited small size (less than 500 nm) using a high concentration of the synthesized chemical compound (NPA, 0.6 g) and good stabilization against agglomeration (more than −30 mv). In addition, NPA-loaded SiO2NPs had no phase separation as observed by our naked eyes even after 30 days. The findings also revealed that the fabricated SiO2NPs could sustain the release of NPA at two different pH levels, 4.5 and 7.4. Additionally, the cell viability of the produced nanoemulsion system loaded with different concentrations of NPA was greater than SiO2NPs without loading, affirming that NPA had a positive impact on increasing the safety and cell viability of the whole nanoemulsion. Based on these obtained promising data, it can be considered that the prepared NPA-loaded SiO2NPs seem to have the potential for use as an effective anticancer drug nanosystem. 相似文献
Summary: Paper describes basic characteristics of synthesis and properties of aliphatic polyesters used for tissue engineering. Described is also synthesis of polyester containing block copolymers suitable for surface modification. Described are methods used for scaffold fabrication with required porosity. In particular, presented are methods according to which scaffolds are made from prefabricated polyester micro- and nanoparticles. 相似文献
Abstract: This article reviews the most recent applications of Raman spectroscopy (RS) in tissue engineering and regenerative medicine (TERM). Raman spectroscopy can potentially alleviate the current lack of capabilities to capture important biological data beyond the standard metabolite and pH indicators in tissue bioreactors. Key process components in bioreactors that are important to evaluate cellular integrity and functionality of tissue-engineered constructs include cells, scaffolds, and proteins. A number of studies are discussed to highlight the capability of RS in noninvasive, nondestructive, and label-free in situ analysis. Protein detection is emphasized due to the urgent need for faster and cheaper techniques. The extensiveness of protein detection studies and the inadequacy of current technology formulate the basis for detailing relevant methods seeking to improve the detection limit, specificity, or multiplexing performance of RS. We argue that RS has great promise to solve the key problem in tissue bioprocessing, namely, inadequate monitoring capabilities that handicap TERM from rising to prevalence in clinical practice. 相似文献
We report the first synthetic route to prepare 3-methyl-4-nitro-5-(2-alkylethenyl)isoxazoles in high yields and exclusively as E-diastereoisomers. 相似文献
Enantioselective catalysis has emerged as a powerful synthetic paradigm and has accelerated the development of new methods to make diverse chiral molecules. Generally, these reactions are very sensitive to the steric and electronic environment present in the catalyst as well as the substrates. With this scenario, the presence of an additional component in the reaction mixture is expected to add complexity in achieving the enantioselective variants. Herein, we report that various enantioenriched molecules could be obtained from multiple starting materials in one pot. The reaction of aminoaromatics A with alkynols B1, B2, B3…?Bn with a AuI/chiral Brønsted acid catalyst afforded AB1*, AB2*, AB3*…ABn*; while, the reaction of alkynols B with aminoaromatics A1, A2, A3…An under the same reaction conditions gave A1B*, A2B*, A3B*…AnB * . 相似文献
Polymeric electrospun meshes are highly attractive as versatile platforms for numerous biomedical applications, tissue engineering, biosensors, and controlled release of bioactive agents. Herein, we describe the preparation and characterization of multilayered nanofibrous poly(ε-caprolactone) scaffolds with different embelin content by electrospinning technique. In vitro release in physiological and acidic pH and kinetic analysis were performed. The results show that it is possible to modulate the release profile depending on the number and thickness of layers added to drug-loaded scaffold that acts as an embelin reservoir. Electrospun multilayered scaffolds present characteristics, morphology and release profiles that could be very attractive for use as embelin controlled release systems. 相似文献