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221.
Yu Tan Lin Zhang Peng Bai Zhilong Li RanRan Zhang Fan Yang Li Wang Weibo Liang 《Electrophoresis》2021,42(9-10):1158-1167
With the discovery of circulating cell-free fetal DNA (cffDNA) in maternal plasma, noninvasive prenatal testing became possible. However, analysis of low-level cffDNA against high background maternal DNA remains complicated and challenging. To circumvent this limitation, selective amplification of cffDNA was used in this study. Two kinds of compound markers (namely DIP-STR and SNP-STR), both based on selective amplification, were used here for targeting fetal DNA. By designing two allele-specific forward primers for DIP-STR and SNP-STR, DNA fragments with different DIP/SNP alleles can be selectively amplified. When analyzing maternal plasma DNA, these markers can selectively target paternally inherited fetal alleles whose DIP/SNP allele was not shared with the mother. In this study, 21 families were studied with six DIP-STRs and 11 SNP-STRs. Fetal DNA was successfully detected across plasma samples for at least one marker. Detection rate varied between DIP-STR and SNP-STR markers, and DIP-STR outperforms SNP-STR. Fetal alleles obtained from maternal plasma were double confirmed by genotyping paternal genomic DNA and fetal genomic DNA from amniocentesis. This study demonstrated that selective amplification strategy can be used to target cffDNA in maternal plasma, which will be a promising method for noninvasive prenatal paternity testing. 相似文献
222.
葡萄糖B-Z体系中酸度变化诱导的复杂振荡反应 总被引:1,自引:0,他引:1
本文首次报导了葡萄糖-KBrO_3-丙酮-MnSO_4-H_2SO_4体系的化学振荡反应,在这一体系中改变酸度可产生一系列复杂的振荡现象,当[H_2SO_4]_0>0.36mol·l~(-1)或[H_2SO_4]_0<0.074mol·l~(-1)时,体系分别出现二种不同类型的振荡波形OA和OB,OA振荡存在一诱导期,OB振荡无锈导期;OA的振幅较小,但振荡频率比OB快得多;OB的振荡周期逐渐缩短,但OA.却相反变化.当0.074mol·l~(-1)<[H_2SO_4]_0<0.36mol·l~(-1)时,体系同时出现上述二种类型的振荡波形,中间存在一过渡区域,即产生连续振荡波形.文章讨论了诱导期及过渡时间与[H_2SO_4]_0的关系,对酸度的影响机理作了说明. 相似文献
223.
饱和烃促进渣油热反应初期生焦的考察 总被引:2,自引:6,他引:2
研究了石油渣油饱和烃的热裂化夺氢化学对渣油热反应体系生焦的影响,对由饱和烃-供氢探针、饱和烃-沥青质组成的二元模型反应体系,以及由饱和烃-供氢探针-沥青质组成的三元模型反应体系,分别进行高压热反应;然后关联二元反应体系和三元反应体系中饱和烃夺氢能力及供氢探针的供氢量和沥青质生焦率。结果表明饱和烃夺氢能力可促进沥青质生焦;并且饱和烃的热裂化夺氢反应性能与饱和烃的物理沉积性能相比较,前者更能促进沥青质形成凝聚相而生焦。进而测定四种减压渣油的热反应生焦趋势,发现渣油饱和分的夺氢量与渣油热反应被期的生焦趋势密切相关,而较苛刻的条件下的生焦趋势主要是由原料渣油残炭值所决定。 相似文献
224.
目的观察并分析降钙素原在指导慢性阻塞性肺疾病急性加重期抗感染治疗中的价值。方法研究对象选取2015年5月期间来湖北省宜昌市第二人民医院就诊的100例慢性阻塞性肺疾病急性加重期病例,根据随机数字表法将其分为对照组与观察组各50例,对照组结合临床实际情况制订抗生素治疗方案,观察组则根据血清降钙素原(PCT)指导抗生素应用,对比两组抗生素应用时间、临床治疗效果、住院时间、病情加重与死亡率,并对研究结果相关数据作统计学处理。结果与对照组比,观察组抗生素使用时间、住院时间更短,组间差异显著而具有统计学意义(P0.05);两组临床治疗效果,病情加重率与死亡率差异并无统计学意义(P0.05)。结论在慢性阻塞性肺疾病急性加重期,监测血清降钙素原水平以指导抗感染治疗可有效降低抗生素使用量,促进患者康复,具有积极的临床意义。 相似文献
225.
单摆作谐振动的最大摆角的分析 总被引:2,自引:0,他引:2
本文根据实验上的微小偏差准则和试验中的最小可分辨时间来确定单摆作谐振动时的最大摆角 相似文献
226.
227.
This paper presents a one-server queueing model with retrials in discrete-time. The number of primary jobs arriving in a time slot follows a general probability distribution and the different numbers of primary arrivals in consecutive time slots are mutually independent. Each job requires from the server a generally distributed number of slots for its service, and the service times of the different jobs are independent. Jobs arriving in a slot can start their service only at the beginning of the next slot. When upon arrival jobs find the server busy all incoming jobs are sent into orbit. When upon arrival in a slot jobs find the server idle, then one of the incoming jobs (randomly chosen) in that slot starts its service at the beginning of the next slot, whereas the other incoming jobs in that slot, if any, are sent into orbit. During each slot jobs in the orbit try to re-enter the system individually, independent of each other, with a given retrial probability. 相似文献
228.
We consider an M/PH/1 queue with workload-dependent balking. An arriving customer joins the queue and stays until served if and only if the system
workload is no more than a fixed level at the time of his arrival. We begin by considering a fluid model where the buffer
content changes at a rate determined by an external stochastic process with finite state space. We derive systems of first-order
linear differential equations for the mean and LST (Laplace-Stieltjes Transform) of the busy period in this model and solve
them explicitly. We obtain the mean and LST of the busy period in the M/PH/1 queue with workload-dependent balking as a special limiting case of this fluid model. We illustrate the results with numerical
examples.
相似文献
229.
In solution, the growth rate and the crystal habit are influenced by a number of factors such as supersaturation, temperature, pH of the solution, cooling rate, agitation, viscosity, initial state of the seed crystal and the presence of impurities. The crystallization of orthorhombic β‐MgSO4 · 7H2O, from low temperature aqueous solution by slow cooling process was studied. The metastable zone width, the induction periods (τ) for different supersaturations and the effect of pH on the growth rate of the crystals were investigated. The increase of pH yielded bigger crystals. The structural, optical, thermal and mechanical properties of β‐MgSO4 · 7H2O have been studied using FT‐IR, X‐ray diffraction, TGA‐DTG and micro hardness analyses. 相似文献
230.