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991.
Petty Sukarsaatmadja 《Tetrahedron letters》2009,50(28):4135-4137
A novel cyclic trimer of imidazole 1, in which imidazole rings are connected by amide bonds, has been synthesized with the help of LiCl as a template for the cyclization. The absorption spectra of 1 indicate the extension of conjugation between imidazole rings and amide bonds. The addition of 3 M equiv of MgCl2 solubilizes 1 in polar organic solvent, suggesting the chelating ability of 1 to Mg cation. 相似文献
992.
M. Monsur Ali Dr. Yingfu Li Prof. Dr. 《Angewandte Chemie (International ed. in English)》2009,48(19):3512-3515
Target detection by the naked eye : The action of an RNA‐cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single‐stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex‐binding dye, the color of the dye changed from blue to purple.
993.
Quantitative methods for food allergens: a review 总被引:1,自引:0,他引:1
Stéphanie Kirsch Séverine Fourdrilis Rowan Dobson Marie-Louise Scippo Guy Maghuin-Rogister Edwin De Pauw 《Analytical and bioanalytical chemistry》2009,395(1):57-67
The quantitative detection of allergens in the food chain is a strategic health objective as the prevalence of allergy continues
to rise. Food allergenicity is caused by proteins either in their native form or in forms resulting from food processing.
Progress in mass spectrometry greatly opened up the field of proteomics. These advances are now available for the detection
and the quantification of traces of allergenic proteins in complex mixtures, and complete the set of biological tests used
until now, such as ELISA or PCR. We review methods classified according to their ability to simultaneously quantify and identify
allergenic proteins and underline major advances in the mass-spectrometric methods.
Stéphanie Kirsch and Séverine Fourdrilis contributed equally to this paper. 相似文献
994.
Diana C. Rambaldi Andrea Zattoni Pierluigi Reschiglian Raffaella Colombo Ersilia De Lorenzi 《Analytical and bioanalytical chemistry》2009,394(8):2145-2149
Self-assembly of the 42-amino-acid-long amyloid peptide Aβ1-42 into insoluble fibrillar deposits in the brain is a crucial event in the pathogenesis of Alzheimer's disease. The fibril
deposition occurs through an aggregation process during which transient and metastable oligomeric intermediates are intrinsically
difficult to be accurately monitored and characterised. In this work, the time-dependent Aβ1-42 aggregation pattern is studied by asymmetrical flow field-flow fractionation with on-line multi-angle light scattering detection.
This technique allows separating and obtaining information on the molar mass (M
r) and size distribution of both the early-forming soluble aggregates and the late prefibrillar and fibrillar species, the
latter having very high M
r. Preliminary results demonstrate that unique information on the dynamic aggregation process can be obtained, namely on the
M
r and size of the forming aggregates as well as on their formation kinetics.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
995.
996.
Adriana Gielbert Linda A. Davis A. Robin Sayers James Hope Andrew C. Gill Maurice J. Sauer 《Journal of mass spectrometry : JMS》2009,44(3):384-396
New forms of transmissible spongiform encephalopathy (TSE) continue to be identified, and consequently sensitive differential diagnosis is increasingly important both for the management of disease in humans and livestock and in providing confidence in the safety of the food chain. TSE diseases are associated with accumulation of protease‐resistant prion protein (PrPSc) and detection of this marker protein is central to diagnosis. Proteolysis by proteinase K (PK) generates protease‐resistant products (PrPres) with partially variable N‐termini. The conformation(s) of PrPSc and thus the points of PK cleavage are thought to be dependent on the strain of prion disease. Western blot (WB) analysis of PrPres gives characteristic migration patterns that can be used to diagnose TSEs, but the relatively low resolution of this technique limits its ability to differentiate certain disease strains. Mass spectrometry (MS) has the capability to resolve these various PK cleavage sites to the level of individual amino acid residues. In the present study multiple selected reaction monitoring (mSRM) was used to detect and quantify PrPres N‐terminal tryptic peptides by MS and thus to define the N‐terminal amino acid profiles (N‐TAAPs) of PrPres characteristic for various TSEs in sheep. The fragmentation behaviour of the N‐terminal tryptic peptides was studied to allow selection of the transitions specific for each peptide. Different PrPres preparation methods were evaluated and the most effective approach applied to differentiate the N‐TAAPs corresponding to various sheep TSE isolates. Marked differences were identified between the N‐TAAPs of bovine spongiform encephalopathy (BSE) and classical scrapie, and between classical scrapie and the experimental strains SSBP/1 and CH1641, thereby validating this approach as a means of TSE‐strain specific diagnosis. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
997.
Shannon L. Cook Olivier L. Collin Glen P. Jackson 《Journal of mass spectrometry : JMS》2009,44(8):1211-1223
Extensive backbone fragmentation resulting in a‐, b‐, c‐, x‐, y‐ and z‐type ions is observed of singly and doubly charged peptide ions through their interaction with a high kinetic energy beam of argon or helium metastable atoms in a modified quadrupole ion trap mass spectrometer. The ability to determine phosphorylation‐sites confirms the observation with previous reports and we report the new ability to distinguish between leucine and isoleucine residues and the ability to cleave two covalent bonds of the proline ring resulting in a‐, b‐, x‐, y‐, z‐ and w‐type ions. The fragmentation spectra indicate that fragmentation occurs through nonergodic radical ion chemistry akin to electron capture dissociation (ECD), electron transfer dissociation (ETD) and electron ionization dissociation mechanisms. However, metastable atom‐activated dissociation mass spectrometry demonstrates three apparent benefits to ECD and ETD: (1) the ability to fragment singly charged precursor ions, (2) the ability to fragment negatively charged ions and (3) the ability to cleave the proline ring that requires the cleavage of two covalent bonds. Helium metastable atoms generated more fragment ions than argon metastable atoms for both substance P and bradykinin regardless of the precursor ion charge state. Reaction times less than 250 ms and efficiencies approaching 5% are compatible with on‐line fragmentation, as would be desirable for bottom‐up proteomics applications. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
998.
František Tureček 《Journal of mass spectrometry : JMS》2010,45(11):1280-1290
Effects of substituted aryl groups on dissociations of peptide aminoketyl radicals were studied computationally for model tetrapeptide intermediates GXD?G where X was a cysteine residue that was derivatized by S‐(3‐nitrobenzyl), S‐(3‐cyanobenzyl), S‐(3,5‐dicyanobenzyl), S‐(2,3,4,5,6‐pentafluorobenzyl), and S‐benzyl groups. The aminoketyl radical was placed within the Asp amide group. Aminoketyl radicals having the S‐(3‐nitrobenzyl) group were found to undergo spontaneous and highly exothermic migration of the hydroxyl hydrogen atom onto the nitro group in conformers allowing interaction between these groups. Competing reaction channels were investigated for aminoketyl radicals having the S‐(3‐cyanobenzyl) and S‐(3,5‐dicyanobenzyl) groups, e.g. H‐atom migration to the C and N atoms of the C≡N group, migration to the C‐4 position of the phenyl ring, and dissociation of the radical‐activated N? Cα bond between the Asp and Gly residues. RRKM kinetic analysis on the combined B3LYP and ROMP2/6‐311++G(2d,p) potential energy surface indicated > 99% H‐atom transfer to the C≡N group forming a stable iminyl intermediate. The N? Cα bond dissociation was negligible. In contrast, peptides with the S‐(2,3,4,5,6‐pentafluorobenzyl) and S‐benzyl groups showed preferential N? Cα bond dissociation that outcompeted H‐atom migration to the C‐4 position and fluorine substituents in the phenyl ring. These computational results are used to suggest an alternative mechanism for the quenching effect on electron‐based peptide backbone dissociations of benzyl groups with electron‐withdrawing substitutents, as reported recently. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
999.
Time‐resolved photodissociation (PD) patterns of singly protonated peptides with arginine at the C‐terminus (C‐arg peptide ions) have been used to classify the dissociation channels into two categories, i.e. high‐energy channels generating v, w and x and low‐energy ones generating b, y and z. x + 1 formed by Cα? CO cleavage seems to be the intermediate ion in high‐energy channels just as a + 1 is for N‐arg peptide ions. Difference in time‐resolved pattern indicates that the two sets of channels, high‐ and low‐energy ones, are not in direct competition. Noncompetitive dissociation is also indicated by the observation of anomalous effect of matrix used in matrix‐assisted laser desorption ionization, a cooler matrix generating more high‐energy product ions both in spontaneous dissociation and in PD. Results from detailed investigation suggest that the two sets of channels start from two (or more) different conformations. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
1000.
We evaluated the hemostatic efficacy of a biological self‐assembling peptide RADA16‐I in a rat kidney injury model. Adult male rats were randomized into five groups: sham operation (no renal excision), no hemostatic agent (control), commercially available gelatin sponge (Gelfoam), 1% RADA16‐I, and 2% RADA16‐I. After left partial nephrectomy, the anesthetized animal was anticoagulated using 300 IU · kg?1 heparin, and the topical hemostatic agent was applied to the injury. Blood loss and mean arterial pressure (MAP) were recorded. As was the case for Gelfoam, 2% RADA16‐I produced marked hemostasis versus controls (p < 0.01). Blood loss with 1% and 2% RADA16‐I was significantly less than controls. The decline in MAP during surgery was less with 2% versus 1% RADA16‐I. RADA16‐I also resulted in less histological tissue responses than Gelfoam. These data suggest that RADA16‐I can stop hemorrhage, with only minimal tissue responses, in experimental renal injury.