用自制酶电极测定牛奶中残留青霉素

用自制酶电极测定牛奶中残留青霉素;生物电化学传感器;青霉素残留     

青霉素裂解液的高效液相色谱分析

 利用高效液相色谱法分析测定青霉素裂解液中的主要成分 6 氨基青霉烷酸 (6 APA)、青霉素G钾和苯乙酸 ,并对副产物青霉噻唑酸进行了液相色谱的定性检测。结果表明 ,实验方法简便、快速 ,结果准确 ,可以用于 6 APA生产的质量控制。     

The engineering and immobilization of penicillin G acylase onto thermo‐sensitive tri‐block copolymer system

In this work, the relationships between catalytic performances of penicillin G acylase (PGA) and the molar ratio of carrier, thermo‐sensitive tri‐block polymer, poly (N,N‐diethylacrylamide‐b‐ β‐hydroxyethyl methacrylate‐b‐glycidyl methacrylate) (PDEA‐b‐PHEMA‐b‐PGMA) were studied firstly, and result documented the optimal molar ratio was n_DEA:n_HEMA:n_GMA = 100:47:24, which presented a suitable lower critical solution temperature (LCST) of 35°C and the activity retention ratio of 80.62% (±0.50%). Based on the suitable carrier, immobilization conditions were investigated and optimized. When pH of solution, concentration of PGA, immobilized time, and immobilization temperature were 8.0, 1/10 (m/v), 16 hours, and 36°C, respectively, enzyme loading capacity (L), enzyme activity (Ea), and activity retention ratio (Ar) of PGA arrived at the highest value of 21 223 U, 16 199 U/g, and 93.50% (±0.50%), respectively. Besides, the response rate (Rr) of immobilized PGA was the same as free PGA, the reusable stability (Rs) was 77.00% (±1.00%) after using for 11 times, which indicated that the carrier has better compatibility with L, Ar, Rs, and Rr.     

某半合成青霉素制药废水的水质指纹特性

近年来,地表水中时有抗生素检出,且浓度较高,因此加强抗生素废水的监管势在必行。三维荧光技术快速、简便且灵敏度高,可以反映有机物组成,其光谱被称为水质指纹。选取某典型半合成青霉素制药废水进行水质指纹特性研究。该废水共有4个主要水纹峰,分别位于激发波长/发射波长为360/445,255/445,275/305和230/300 nm附近。在一定浓度范围内,各峰强度与污染物浓度呈现良好的线性关系。该废水的两个峰275/305和230/300 nm可能源于产品中间体左旋对羟基苯甘氨酸邓钾盐、产品阿莫西林和水解产物左旋对羟基苯甘氨酸等。pH对水质指纹有明显影响,这显示出某些荧光污染物可能具有酸碱基团。水质指纹能够用于该种抗生素制药废水的监测。     

青霉素酰化酶在介孔分子筛MCM-41上的固定化研究

通过改变酶固定化时的pH值、温度、时间以及酶用量,研究了固定化条件对MCM-41载体上固定化青霉素酰化酶的酶活及其稳定性的影响,明确了酶活及其稳定性随固定化条件的变化规律,初步分析了青霉素酰化酶在介孔分子筛MCM-41上的固定化过程。     

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

Immobilized penicillin G acylase (PGA) as an important industrial catalyst can catalyze penicillin G potassium (PG) to 6‐aminopenicillanic acid (6‐APA). 6‐APA is an important intermediate for semisynthetic penicillin drugs, which occupies a huge market space in the anti‐inflammatory field; as a result, immobilized PGA occupies a huge market space in the pharmaceutical field. However, at present, there are different degrees of defects in the preparation and production process of immobilized PGAs on the market because of the huge demand; therefore, the performance of immobilized PGA and its productivity will bring huge economic benefits to enterprises. Therefore, research on immobilized PGA has always been a focus. This review first introduces the source, classification, structure, and catalytic mechanism of PGA and then studies the development of immobilization methods, immobilized carriers, reaction media, enzyme activity regeneration, and reactors of immobilized PGA in recent years.     

微波辐射高效共价固定青霉素酰化酶

为提高青霉素酰化酶的共价固定化效率, 在微波辐射条件下将酶蛋白共价固定于介孔泡沫硅(MCFs)的孔道中. 通过正硅酸四乙酯水解缩合制备介孔泡沫硅, 再于微波辅助下将青霉素酰化酶共价固定在其孔道中. 以固定化酶相对活力和活力回收为指标, 考察了加酶量、固定化温度、微波辐射时间等条件对酶固定化效率的影响. 实验结果表明: 当加酶量为60 mg/g, 固定化温度为20 ℃, 微波辐射140 s, 固定化酶相对活力达到178.1%, 表观活力为1191.3 U/g(以湿重计). 与常规方法相比, 微波辅助固定化酶时, 固定化酶相对活力提高34.5%, 固定化时间亦大幅缩短至数分钟, 这为青霉素酰化酶的高效共价固定化提供了一条新的途径.