全文获取类型
收费全文 | 223篇 |
免费 | 30篇 |
国内免费 | 11篇 |
专业分类
化学 | 261篇 |
物理学 | 3篇 |
出版年
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 8篇 |
2020年 | 17篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 13篇 |
2015年 | 15篇 |
2014年 | 7篇 |
2013年 | 15篇 |
2012年 | 10篇 |
2011年 | 14篇 |
2010年 | 8篇 |
2009年 | 13篇 |
2008年 | 15篇 |
2007年 | 16篇 |
2006年 | 17篇 |
2005年 | 19篇 |
2004年 | 16篇 |
2003年 | 15篇 |
2002年 | 6篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 5篇 |
1994年 | 1篇 |
1990年 | 1篇 |
排序方式: 共有264条查询结果,搜索用时 15 毫秒
21.
Nathalie Guarnizo Diego Oliveros Walter Murillo-Arango María Bianney Bermúdez-Cardona 《Molecules (Basel, Switzerland)》2020,25(24)
Plants have innate immune systems or defense mechanisms that respond to the attack of pathogenic microorganisms. Unlike mammals, they lack mobile defense cells, so defense processes depend on autonomous cellular events with a broad repertoire of recognition to detect pathogens, which compensates for the lack of an adaptive immune system. These defense mechanisms remain inactive or latent until they are activated after exposure or contact with inducing agents, or after the application of the inductor; they remain inactive only until they are affected by a pathogen or challenged by an elicitor from the same. Resistance induction represents a focus of interest, as it promotes the activation of plant defense mechanisms, reducing the use of chemical synthesis pesticides, an alternative that has even led to the generation of new commercial products with high efficiency, stability and lower environmental impact, which increase productivity by reducing not only losses but also increasing plant growth. Considering the above, the objective of this review is to address the issue of resistance induction with a focus on the potential of the use of oligosaccharides in agriculture, how they are recognized by plants, how they can be used for commercial products and perspectives. 相似文献
22.
Juyeol Lee Jihun Kang Sukhyun Lee Young Ho Rhee 《Angewandte Chemie (International ed. in English)》2020,59(6):2349-2353
A de novo first collective total synthesis of 11‐deoxylandomycins is reported. A signature step is featured by the Pd‐catalyzed asymmetric addition of alcohol to ene‐alkoxyallenes that assembles oligomeric 2,3,6‐trideoxyoligosaccharides. The unique feature of the protocol is illustrated by a flexible access to various natural 11‐deoxylandomycins as well as non‐natural analogues. 相似文献
23.
Liwen Han 《Journal of carbohydrate chemistry》2013,32(5-6):335-382
AbstractLipoarabinomannan (LAM), mannosyl LAM (ManLAM), and mycolyl-arabinogalactan (mAG) are unique and ubiquitous cell wall constituents of Mycobacterium tuberculosis (M. tb), the bacterium causing tuberculosis (TB). It has been well documented that LAM, ManLAM, and mAG play an important role in mycobacterial infections and in the elicitation of specific immune responses against M. tb in the host. Therefore, LAM, ManLAM, mAG, and related molecules are attractive targets for the development of novel TB diagnostic and therapeutic strategies. Accordingly, numerous research groups have spent great effort on the chemical synthesis and biological studies of mycobacterium-related arabinofuranosyl oligosaccharides and their mimetics and conjugates. This article provides an extensive review about the progresses in this area. Due to the page limit of this journal, the review is published in three parts separately. This part (Part II) is focused on the synthesis of various ManLAM and mAG analogs containing mannose, galactose or galactosamine units and other natural structural motifs, as well as arabinofuranosyl oligosaccharide C-analogs and other derivatives. 相似文献
24.
We examined the utility of a monolithic capillary column in the analysis of pyridylaminated oligosaccharides. Fluorescence detection and mass spectrometry were used to monitor a series of oligosaccharides. Although the total-ion chromatogram appeared similar to that obtained with fluorescence detection, the sensitivity of this technique was limited, especially in the case of smaller oligosaccharides. This limitation was overcome by applying selected ion current monitoring. Further, the capillary column also exhibited good reproducibility. We showed that the retention times obtained by using the monolithic capillary column could be converted into the standard data to enable comparison of the experimental data with the existing data. Furthermore, our studies revealed an important difference in the separation profile, i.e., the monolithic capillary column could resolve smaller oligosaccharides to a greater extent. 相似文献
25.
26.
Zhen Wang Yongmei Xu Bo Yang Gopinath Tiruchinapally Bin Sun Renpeng Liu Steven Dulaney Jian Liu Xuefei Huang 《Chemistry (Weinheim an der Bergstrasse, Germany)》2010,16(28):8365-8375
Heparin (HP) and heparan sulfate (HS) play important roles in many biological events. Increasing evidence has shown that the biological functions of HP and HS can be critically dependent upon their precise structures, including the position of the iduronic acids and sulfation patterns. However, unraveling the HP code has been extremely challenging due to the enormous structural variations. To overcome this hurdle, we investigated the possibility of assembling a library of HP/HS oligosaccharides using a preactivation‐based, one‐pot glycosylation method. A major challenge in HP/HS oligosaccharide synthesis is stereoselectivity in the formation of the cis‐1,4‐linkages between glucosamine and the uronic acid. Through screening, suitable protective groups were identified on the matching glycosyl donor and acceptor, leading to stereospecific formation of both the cis‐1,4‐ and trans‐1,4‐linkages present in HP. The protective group chemistry designed was also very flexible. From two advanced thioglycosyl disaccharide intermediates, all of the required disaccharide modules for library preparation could be generated in a divergent manner, which greatly simplified building‐block preparation. Furthermore, the reactivity‐independent nature of the preactivation‐based, one‐pot approach enabled us to mix the building blocks. This allowed rapid assembly of twelve HP/HS hexasaccharides with systematically varied and precisely controlled backbone structures in a combinatorial fashion. The speed and the high yields achieved in glycoassembly without the need to use a large excess of building blocks highlighted the advantages of our approach, which can be of general use to facilitate the study of HP/HS biology. As a proof of principle, this panel of hexasaccharides was used to probe the effect of backbone sequence on binding with the fibroblast growth factor‐2 (FGF‐2). A trisaccharide sequence of 2‐O‐sulfated iduronic acid flanked by N‐sulfated glucosamines was identified to be the minimum binding motif and N‐sulfation was found to be critical. This provides useful information for further development of more potent compounds towards FGF‐2 binding, which can have potential applications in wound healing and anticancer therapy. 相似文献
27.
Gil Garrote Herminia Domínguez Juan C. Parajó 《Applied biochemistry and biotechnology》2001,95(3):195-207
Milled corncob samples were mixed with water and heated to obtain a liquid phase containing oligosaccharides, sugars, and
acetic acid as main reaction products (autohydrolysis reaction). To hydrolyze the sugar oligomers to the correspondent monomers,
sulfuric acid was added to the autohydrolysis liquors to reach 0.5–2 wt% of solution, and the reaction media were heated at
101.5–135°C. With this operational procedure, sugar solutions suitable as fermentation media (containing xylose as the major
component) were obtained. The kinetics of the posthydrolysis step was characterized on the basis of experimental data concerning
the time courses of the concentrations of xylooligosaccharides, xylose, furfural, and acetic acid. The concentrations of other
reaction byproducts (glucose or arabinose) were also measured. 相似文献
28.
Chemical Synthesis Elucidates the Immunological Importance of a Pyruvate Modification in the Capsular Polysaccharide of Streptococcus pneumoniae Serotype 4
下载免费PDF全文
![点击此处可从《Angewandte Chemie (International ed. in English)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Dr. Claney L. Pereira Andreas Geissner Dr. Chakkumkal Anish Prof. Dr. Peter H. Seeberger 《Angewandte Chemie (International ed. in English)》2015,54(34):10016-10019
Carbohydrate modifications are believed to strongly affect the immunogenicity of glycans. Capsular polysaccharides (CPS) from bacterial pathogens are frequently equipped with a pyruvate that can be placed across the 4,6‐, 3,4‐, or 2,3‐positions. A trans‐2,3‐linked pyruvate is present on the CPS of the Gram‐positive bacterium Streptococcus pneumoniae serotype 4 (ST4), a pathogen responsible for pneumococcal infections. To assess the immunological importance of this modification within the CPS repeating unit, the first total synthesis of the glycan was carried out. Glycan microarrays containing a series of synthetic antigens demonstrated how antibodies raised against natural ST4 CPS specifically recognize the pyruvate within the context of the tetrasaccharide repeating unit. The pyruvate modification is a key motif for designing minimal synthetic carbohydrate vaccines for ST4. 相似文献
29.
《化学:亚洲杂志》2017,12(10):1027-1042
The importance of carbohydrates is evident by their essential role in all living systems. Their syntheses have attracted attention from chemists for over a century. Most chemical syntheses in this area focus on the preparation of carbohydrates from naturally occurring monosaccharides. De novo chemical synthesis of carbohydrates from feedstock starting materials has emerged as a complementary method for the preparation of diverse mono‐ and oligosaccharides. In this review, the history of de novo carbohydrate synthesis is briefly discussed and particular attention is given to methods that address the formation of glycosidic bonds for potential de novo synthesis of oligosaccharides. Almost all methods of this kind involve the formation of dihydropyran intermediates. Recent progress in forming dihydropyrans by Achmatowicz rearrangement, hetero‐Diels–Alder cycloaddition, ring‐closing metathesis, and other methods is also elaborated. 相似文献
30.
Erdélyi M d'Auvergne E Navarro-Vázquez A Leonov A Griesinger C 《Chemistry (Weinheim an der Bergstrasse, Germany)》2011,17(34):9368-9376
The dynamics of the glycosidic bond of lactose was studied by a paramagnetic tagging‐based NMR technique, which allowed the collection of an unusually large series of NMR data for a single compound. By the use of distance‐ and orientation‐dependent residual dipolar couplings and pseudocontact shifts, the simultaneous fitting of the probabilities of computed conformations and the orientation of the magnetic susceptibility tensor of a series of lanthanide complexes of lactose show that its glycosidic bond samples syn/syn, anti/syn and syn/anti ?/ψ regions of the conformational space in water. The analysis indicates a higher reliability of pseudocontact shift data as compared to residual dipolar couplings with the presently available weakly orienting paramagnetic tagging technique. The method presented herein allows for an improved understanding of the dynamic behaviour of oligosaccharides. 相似文献