Prototypical Nonelectrochemical Method for Surface Regeneration of an Integrated Electrode in a PDMS Microfluidic Chip

Abstract

A prototypical method for surface regeneration of an integrated electrode in a microfluidic chip is demonstrated. A platinum wire as working electrode was mounted in a polydimethylsiloxane chip vertical to the chip through the channel. The regeneration of the electrode was easily achieved by drawing the platinum wire out for 5 mm, because the area exposed to the channel or the stream would be altered. With continuous motion, the wire electrode can maintain a fresh surface just like a dropping mercury electrode. The current–time curve and open circuit potential (OCP) of dopamine solution show the performance of this prototypical system.     

Advanced Immobilization and Amplification for High Performance Protein Chips

With the success of high-throughput DNA microarrays, protein biochips have been intensively investigated and broadly used in bioscience research, clinic diagnosis, drug discovery, and other applications. However, there is great need to significantly improve the sensitivity of protein chips, especially in early diagnosis. A major challenge of improving sensitivity is that protein detection does not have an effective amplification method, such as PCR for DNA microarrays. Construction of unique biofilms for efficient immobilization of protein probes and innovation of new amplification schemes could play a critical role in performance improvement of protein biochips. With dramatic developments in microfabrication, nanotechnologies, and biotechnologies, enormous progress has been made, particularly in improving biosensing sensitivity. This article reviews new advances in protein biochip technologies with emphasis on novel approaches for efficient probe immobilization and nanomaterials-assisted signal amplification for high performance protein chips. Prominent progress in integration of protein microarrays with microfluidic platforms is briefly discussed. The major challenges and perspectives on the future of protein biochips are also addressed.     

Effect of the aging on lees and other alternative techniques on the low molecular weight phenols of Tempranillo red wine aged in oak barrels

The effect of different alternative techniques to the traditional aging on lees on the low molecular weight phenolic compounds of red wines was study as well as their evolution during the aging in oak wood barrels for six months. The study was carried out with Tempranillo red grapes from two consecutive vintages. The techniques assayed were the traditional aging on lees with or without the addition of exogenous β-glucanase enzymes, the use of yeast derivative preparations also with or without the addition of exogenous β-glucanase enzymes, the micro-oxygenation applied together with the aging on lees, and the use of non-toasted oak wood chips.     

微通道中应用"流动汇聚"形成分散体系

在微流控器件内集成具有流动汇聚功能的几何结构以研究液--液系统中液滴的形成. 相图显示液滴的大小为流量以及两种液体流量比的函数, 它包括两个区域, 在一个区域中液滴大小和孔隙宽度接近, 而在另一区域液滴大小则取决于``汇聚'后的细流直径, 从而能形成远小于孔隙的液滴. 单分散和多分散乳状液均可以被生成.     

基于图像的磨粒在线监测系统关键技术研究

针对磨粒在线监测系统开发要求,对其中的关键技术进行了深入研究.构建了基于显微图像分析和微泵进样的磨粒在线监测系统,给出了图像检测子系统和微流控分析芯片的详细设计原理和方法;利用粗糙集理论对磨粒特征参数进行了优化;根据系统光路特点,对磨粒图像进行了基于彩色特征的转换,并通过与背景图像的差值处理来快速提取磨粒目标.最后,利用颗粒计数器和铁谱分析技术对系统性能进行了检验,结果表明本系统磨粒统计与识别准确率分别达到90%和95%,具有较高的检测精度,满足磨粒在线监测要求.     

Fire Retarded Insulating Sheets from Recycled Materials

In this work we aimed at forming partially recycled polymer composites of appropriate mechanical properties and flame retardancy. Multilayer composite structures proved to be suitable to fulfill all of these requirements. Core-shell structures presented here contain two-component thermosets, i.e. epoxy, recycled polyurethane and polyisocyanurate, as matrices reinforced with waste fillers such as short basalt fibers and wood chips. Flame retardancy and mechanical properties of the core-shell structures were investigated by the conventional methods of characterization. The developed cost-effective multilayer composites can be applied as heat and sound insulating panels e.g. multifunctional sheets for construction or automotive industry.     

微流控芯片毛细管电泳在蛋白质分离分析中的应用研究进展

重点介绍了近年来国内外在微流控芯片毛细管电泳法用于蛋白质分离分析方面的研究进展。按照分离模式的不同,综述了各种应用于蛋白质分离的微流控芯片毛细管电泳系统,讨论了抑制芯片中的蛋白吸附的各种方法,并展望了芯片毛细管电泳系统在蛋白质分离领域的发展前景。引用文献47篇。     

微流控纸芯片在环境分析检测中的应用

近年来,微流控纸芯片由于低成本、便携化、检测快等优点,在需要快速检测的环境分析领域中展现出了巨大的应用前景.该综述从微流控纸芯片在环境分析中的应用角度,总结归纳了微流控纸芯片在环境分析中的最新研究进展,并展望了其在未来的发展趋势与挑战.论文内容引用150余篇源于科学引文索引(SCI)与中文核心期刊中的相关论文.该综述包...     

基于微流控技术的细胞水平高通量药物筛选系统的研究进展

药物筛选是新药研发的关键步骤,创新药物的发现需要采用适当的药物作用靶点对大量化合物样品进行筛选.高通量筛选系统能够实现数千个反应同时测试和分析,大大提高了药物筛选的实验规模和效率.其中基于细胞水平的高通量药物筛选系统因为更加接近人体生理条件,成为主要的筛选模式.而目前发展成熟的高通量细胞筛选系统主要基于多孔板,存在细胞...