甜菜糖蜜酒精废液生产菌体蛋白饲料初探

甜菜糖蜜酒精废液含有大量的有机物，pH值低，BOD、CDO值高，直接排和会造成环境的严重污染，又损失了一些有机物，在此废液中可直接接入酵母菌，使其大量繁殖，生产出的菌体粗蛋白含量达41．7％，CDO值降低了20％以上，减轻了环境污染。     

Pseudo cyclic ionophores: ‘Binary-effect’ of quinolinyloxy groups at both ends of oligoethylene glycols on the conformational stabilization of their complexes with alkali metal salts

A series of noncyclic neutral ionophores has been synthesized by the reaction of oligoethylene glycol dihalides with 8-quinolinol. Complexation properties for alkali metal picrates were evaluated from solvent extraction and bulk liquid membrane transport experiments. Complexation profiles of the newly synthesized ionophores with a hexyl chain were similar to those of their homologues without the hexyl chain in the extraction experiments. Among them, the pentaethylene glycol derivatives showed the highest extraction efficiency and selectivity towards potassium ion. From the¹H NMR spectra (400 MHz), the change in chemical shifts of the aromatic protons upon the addition of alkali metal thiocyanates suggested the existence of a stabilization effect which is caused by intramolecular stacking conformations between the quinoline rings during complexation. Aryl stacking interactions depend on the size of the cations and on the chain length of the oligoethylene glycol. The relationship between transport ability towards alkali metal cations and lipophilicity of these ionophores is also discussed.     

Fluorescence anisotropy decay study of self-association of bacterial luciferase intermediates

The fluorescence dynamics parameters of the fluorescent transient flavin-luciferase species from the typesVibrio fischeri andPhotobacterium leiognathi are presented. The fluorescence anisotropy decay is a single exponential function for both types. The correlation time is 70 ns for theP. leiognathi fluorescent transient intermediate (2°C, aqueous buffer, pH 7.0), consistent with the rotational correlation time of the luciferase macromolecule (77 kD) to which the flavin fluorophore is rigidly attached. In contrast, for theV. fischeri species the observed correlation time for the anisotropy decay function is 133 ns. This suggests that protein self-association occurs in theV. fischeri case and this is confirmed by filtration, where the fluorescent transient fromV. fischeri does not pass through a 100,000 molecular weight cutoff membrane, whereas theP. leiognathi species does. The filtration method also demonstrates self-association in the luciferase peroxyflavin and photoflavin fromV. fischeri. A monomer-dimer equilibrium also explains the previously reported high correlation times for theV. harveyi luciferase-flavin species. It is proposed that the self-association competes with the lumazine protein interaction in the bioluminescence reaction.     

Altered protein synthesis in rat kidney cells exposed to semiconductor materials

It is thought that the extensive industrial use of arsenic, gallium and indium, which have applications as the materials for III–V semiconductors, will increase human exposure to these compounds in the near future. We have undertaken the development of new biological indicators for assessing exposure to these elements. Element-specific alterations in protein synthesis patterns were expected to occur following exposure to arsenic compounds. We examined alterations in protein synthesis in primary cultures of rat kidney proximal tubule epithelial cells by sodium arsenite, gallium chloride and indium chloride, utilizing two-dimensional gel electrophoresis. After incubation with the chemicals for 20 h, newly synthesized proteins were labeled with [³⁵S]methionine. A protein with a molecular weight (M_r) of 30 000 was markedly induced on exposure to 10 μM arsenite or 300 μM gallium chloride, and synthesis of proteins with M_r values of 85 000, 71 000, 65 000, 51 000, 38 000 and 28 000 were also increased by exposure to arsenite and gallium chloride. No significant changes were observed upon exposure to indium. Some of these increased proteins could be heat-shock proteins.     

改性壳聚精膜用于醇水混合液分离的研究──Ⅲ．超细粉末填充壳聚精膜

首次制备了用Ｓｉ３Ｎ４，ＳｉＯ２，ＴｉＨ４超细粉末填充的改性壳聚精膜，并将其用于乙醇／水混合液的分离中。实验表明壳聚糖膜经超细粉末填充后强度增大，溶胀度减小。用于乙醇／水混合液渗透蒸发分离时，分离因子和渗透通量都有提高，并在填充量为１６．７％附近出现极大值。随着料液中乙醇含量的增大，渗透通量减小，分离因子增大；随着料液温度的升高渗透通量显著增大，分离因子则稍有下降。并简单讨论了超细粉末在乙醇／水混合液分离中的作用。     

铸膜液溶剂体系的物理参数与BCA不对称膜的透气性

铸膜液的溶剂体系（溶剂和非溶剂）对不对称膜形态的形成有重要作用。为了探索溶剂和透气性间的关系，本文考虑到了制备ＢＣＡ梯度密度不对称膜的各种溶剂的一系列物理参数，得到了一些定性结果，并指出了更全面、明确、解释两者关系的研究方向。     

Recognition of Monohydrogen Anions of Geometrical and Positional Isomers of Dicarboxylic Acids Based on Polymeric Liquid Membranes Incorporating Polyamine Host

Macrocyclic lipophilic polyamines were applied as the sensory elements of polymeric liquid membrane electrodes. These hosts gain the anion receptor functionality upon protons uptake from the aqueous solutions. The electrodes studied were able to distinguish potentiometricaly isomers of monocharged and doublecharged forms of ethylene and benzene dicarboxylic acids. The selectivity of interaction between protonated hosts and anionic guests relays not only on the electrostatic interactions, but on hydrogen bounds formations as well. The influence of lipophilicity of protonated hosts on the potential response generation was discussed.     

Taura 综合征病毒主要结构蛋白的表达与纯化

根据Taura综合征病毒(TSV)基因组,设计特异性引物,从感染病毒组织中提取组织总RNA后扩增,分别将3个主要结构蛋白基因VP1、VP2和VP3克隆到pGEM TEasyVector.与表达载体连接后,导入大肠杆菌中诱导表达,并纯化目的蛋白.诱导表达的融合蛋白分子量分别为54.2×103、43×103和57.1×103,在变性条件下过柱纯化VP1和VP2,一次可以纯化10mg以上纯度较高的蛋白.     

槲寄生蛋白注射液细胞毒活性测定的MTT方法探讨

采用MTT比色法对体外培养的肿瘤细胞进行细胞毒作用实验，验证槲寄生蛋白注射液体外抗肿瘤效果．并且鉴定这种检测方法的有效性。实验结果表明槲寄生蛋白注射液有一定的体外抗肿瘤效果；MTT比色法是一种可用的体外细胞毒作用检测法，为新药品的开发提供了实验依据。     

核酸识体的研究及应用

核酸识体(aptamer)是近年来发展起来的一类经体外人工进化程序筛选出的寡聚核苷酸。它能高效、特异地结合各种配体，在蛋白质的分析检测、医学诊断治疗、生物传感器和分子开关的开发等方面有很大的应用前景，文章对核酸识体的研究和应用进展进行了综述。