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11.
Use of specific polysaccharide‐immobilized monodisperse poly(glycidyl methacrylate) core–silica shell microspheres for affinity purification of lectins 下载免费PDF全文
Volodymyr Antonyuk Silvia Grama Zdeněk Plichta Iryna Magorivska Daniel Horak Rostyslav Stoika 《Biomedical chromatography : BMC》2015,29(5):783-787
Immobilization of polysaccharides (yeast mannan and gum arabic) on the macroporous poly(glycidyl methacrylate) monodisperse microspheres coated with silica (SiO2)‐containing amino groups on the surface was used to prepare affinity sorbents for lectin purification. The efficiency of isolating mannose specific Pisum sativum lectin was demonstrated on sorbent with immobilized yeast mannan and that of galactose specific Glycine hispida lectin on sorbent with immobilized gum arabic. The microspheres with immobilized polysaccharides can be used for selecting an affinity sorbent for purification of other mannose‐ and galactose‐specific lectins. In contrast to yeast mannan, the gum arabic immobilized on the microspheres possesses much narrower specificity and is suitable for purification of only those galactose specific lectins which interact well with l ‐rhamnose or l ‐arabinose. The synthesized macroporous particles are capable of immobilizing 50 mg of polysaccharide per 1 g of the matrix, which is 10 times higher than the capacity of epoxy‐activated Sepharose 6B. That makes it possible to obtain the same lectin quantity using a column of 10 times smaller volume. Another advantage of novel affinity sorbents comparing corresponding Sepharose gels is the possibility of sorbent drying after use. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
12.
酵母甘露聚糖硫酸酯化前后结构与生物活性比较研究 总被引:5,自引:2,他引:3
对酵母甘露聚糖及硫酸酯化酵酯母甘露聚糖的相对分子质量、红外光谱、离子色谱、比旋光度、IO4^-氧化、SO4^2-含量、Smith降解、电泳及THP-1细胞分泌IL-la的水平等进行了测定,结果表明,硫酸酯化后相对分子质量增加,IR光谱[α]D^20均有明显变化,电泳迁移加快,IO4^-氧化甲酸生成量减少,硫酸酯化位置在非还原末端,C(3)对碱位置稳定,Smith降解结果表明硫酸化前后基本结构无明显变化。硫酸酯化前后样品对THP-1细胞分泌IL-1a水平影响差异显,对IL-6,IL-8,TNFa,IL-2亦有影响。 相似文献
13.
Ramírez HL Cao R Fragoso A Torres-Labandeira JJ Dominguez A Schacht EH Baños M Villalonga R 《Macromolecular bioscience》2006,6(7):555-561
Mannan and carboxymethylcellulose, previously activated by periodate oxidation, were grafted with mono-6-butylenediamino-6-deoxy-beta-cyclodextrin derivatives by reductive alkylation in the presence of sodium borohydride. The formation of supramolecular complexes between these polymers and Naproxen was confirmed by fluorescence spectroscopy. The solubility of the drug was 3.8-4.6 fold increased in the presence of the cyclodextrin-grafted polysaccharides. The in vivo anti-inflammatory property of Naproxen was 1.7 times higher after supramolecular association with beta-cyclodextrin-branched mannan. 相似文献