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81.
植物凝集素的研究进展 总被引：14，自引：0，他引：14
综述了有关植物凝集素的研究进展,介绍了植物凝集素的分类、糖结合特性、近年来有关植物凝集素蛋白晶体结构的研究,以及其与糖结合能力相关的生物学功能, 相似文献
82.
LIU Jia-Ming LIU Zhen-Bo LI Zhi-Ming HE Hang-Xia LIN Wei-Nü HUANG Ya-Hong WANG Fang-Mei 《中国化学》2008,26(9):1635-1640
In the presence of heavy atom perturber Pb2＋, silicon dioxide nanoparticle containing fluorescein isothiocyanate (FITC-SiO2) could emit a strong and stable room temperature phosphorescence (RTP) signal on the surface of acetyl cellulose membrane (ACM). It was found in the research that a quantitative specific affinity adsorption (AA) reaction between triticum vulgare lectin (WGA) labeled with luminescent nanoparticle and glucose (G) could be carried on the surface of ACM. The product (WGA-G-WGA-FITC-SiO2) of the reaction could emit a stronger RTP signal, and the ΔIp had linear correlation to the content of G. According to the facts above, a new method to determine G by affinity adsorption solid substrate room temperature phosphorimetry (AA-SS-RTP) was established, based on WGA labeled with FITC-SiO2. The detection limit (LD) of this method calculated by 3Sb/k was 0.47 pg•spot－1 (corresponding to a concentration value 1.2×10－9 g•mL－1, namely 5.3×10－9 mol•L－1), the sensitivity was high. Meanwhile, the mechanism for the determination of G by AA-SS-RTP was discussed. 相似文献
83.
刘殿功;梅连青;吴传京 《武汉大学学报(理学版)》1989,(2):105-111
本文报导以武昌产蚕豆为材料,经抽提,分离纯化而得到高纯度的蚕豆凝集素VFL),经PAGE后,用考马斯亮兰液染色呈一条谱带,用高碘酸—Schiff试液染色呈阳性反应。用Bio-Gel P-100凝胶层析测定出VFL的表观分子量为46 000左右,经SDS—PAGE和6M盐酸胍—Sepharose 6B凝胶过滤测定,VFL的亚基分子量为23 600。VFL完整分子可能是由两个相同或相近的亚基组成。用凝胶电聚焦祛测定VFL的PI值为6.25。用DNS—Cl法和高压液相测定VFL的N-末端氨基酸为亮氨酸。VFL对兔红细胞、人的四种血型红细胞均有凝集作用,但对AB型红细胞凝集作用较弱。 相似文献
84.
ZHU Qian-kun ZHU Meng-li ZOU Jia-xin FENG Pei-chun FAN Gao-tao LIU Zu-bi WANG Wan-jun 《高等学校化学研究》2013,29(6):1153-1158
Lycoris radiata mannose-binding lectin（LRL） is a protein which binds mannose residues specifically. The maturation peptide and three mannose-binding domains（residues 49-57, 80-88 and 113--121） of LRL were identified by sequence analysis. The 3D structure of LRL constructed by homology modeling shaped a flstular triangular prism. Three flanks of the prism are mainly composed of β-sheets and each flank has a mannose-binding domain. According to the docking and dynamics simulation, the bindings of residues 49--57 and 80--88 with mannose are more stable than that of residues 113--121 with it. The key residues for binding mannose are Gin80, Asp82, Ash84 and Tyr88. The study preliminarily analyzed the interaction sites and mechanism of LRL with mannoses, which could be useful for the study on insect-resistance and related drug discovery of LRL. 相似文献
85.
发展了一种新型的磁性纳米粒子应用于人血清中特异性糖蛋白的亲和富集。制备的磁性纳米粒子具有核/壳/壳结构,即由Fe3O4磁性粒子/硅胶层/有机聚合物外层构成。伴刀豆凝集素A（Con A）以共价键合的形式通过短链聚乙二醇固定在粒子表面,实现了人血清中特异性糖蛋白的高效富集。富集的蛋白经过胰蛋白酶酶解后,所得的肽段经离线的二维色谱分离,用高分辨质谱共鉴定出80种蛋白。通过NetNGlyc等搜索软件分析确定其中76种为糖蛋白,分析发现在血清中质量浓度仅为0.00001 g/L的 β -2-glycoprotein 1也得到了鉴定,表明我们发展的磁性纳米粒子与凝集素相结合的方式,可以高效地富集复杂体系中与主要蛋白成分含量相差12个数量级的低丰度糖蛋白。 相似文献
86.
通过一种简易的方法,利用D-半乳糖胺和氯金酸制备出了能够用于肝癌细胞靶向识别的Au纳米颗粒探针.该纳米颗粒形貌和尺寸均一并且生物相容性良好.通过改变反应体系的pH能够对Au纳米颗粒的尺寸进行调控.此外,这种新型的纳米颗粒对RCA120还具有超高的检测灵敏度,实验结果显示其检测限度可以达到2μg·L^-1. 相似文献
87.
Sarkar M. Abe Kawsar Imtiaj Hasan Sultana Rajia Yasuhiro Koide Yuki Fujii Ryuhei Hayashi Masao Yamada Yasuhiro Ozeki 《Molecules (Basel, Switzerland)》2021,26(16)
Lectins facilitate cell–cell contact and are critical in many cellular processes. Studying lectins may help us understand the mechanisms underlying tissue regeneration. We investigated the localization of an R-type lectin in a marine annelid (Perinereis sp.) with remarkable tissue regeneration abilities. Perinereis nuntia lectin (PnL), a galactose-binding lectin with repeating Gln-X-Trp motifs, is derived from the ricin B-chain. An antiserum was raised against PnL to specifically detect a 32-kDa lectin in the crude extracts from homogenized lugworms. The antiserum detected PnL in the epidermis, setae, oblique muscle, acicula, nerve cord, and nephridium of the annelid. Some of these tissues and organs also produced Galactose (Gal) or N-acetylgalactosamine (GalNAc), which was detected by fluorescent-labeled plant lectin. These results indicated that the PnL was produced in the tissues originating from the endoderm, mesoderm, and ectoderm. Besides, the localizing pattern of PnL partially merged with the binding pattern of a fluorescent-labeled mushroom lectin that binds to Gal and GalNAc. It suggested that PnL co-localized with galactose-containing glycans in Annelid tissue; this might be the reason PnL needed to be extracted with haptenic sugar, such as d-galactose, in the buffer. Furthermore, we found that a fluorescein isothiocyanate-labeled Gal/GalNAc-binding mushroom lectin binding pattern in the annelid tissue overlapped with the localizing pattern of PnL. These findings suggest that lectin functions by interacting with Gal-containing glycoconjugates in the tissues. 相似文献
88.
Mauro Marengo Aristodemo Carpen Gianfranco Mamone Pasquale Ferranti Stefania Iametti 《Molecules (Basel, Switzerland)》2022,27(9)
Selected food proteins may represent suitable markers for assessing either the presence/absence of specific food ingredients or the type and intensity of food processes. A fundamental step in the quantification of any protein marker is choosing a proper protocol for solubilizing the protein of interest. This step is particularly critical in the case of solid foods and when the protein analyte is prone to undergo intermolecular disulfide exchange reactions with itself or with other protein components in the system as a consequence of process-induced unfolding. In this frame, gluten-based systems represent matrices where a protein network is present and the biomarker proteins may be either linked to other components of the network or trapped into the network itself. The protein biomarkers considered here were wheat gluten toxic sequences for coeliac (QQPFP, R5), wheat germ agglutinin (WGA), and chicken egg ovalbumin (OVA). These proteins were considered here in the frame of three different cases dealing with processes different in nature and severity. Results from individual cases are commented as for: (1) the molecular basis of the observed behavior of the protein; (2) the design of procedure aimed at improving the recovery of the protein biomarker in a form suitable for reliable identification and quantification; (3) a critical analysis of the difficulties associated with the plain transfer of an analytical protocol from one product/process to another. Proper respect for the indications provided by the studies exemplified in this study may prevent coarse errors in assays and vane attempts at estimating the efficacy of a given treatment under a given set of conditions. The cases presented here also indicate that recovery of a protein analyte often does not depend in a linear fashion on the intensity of the applied treatment, so that caution must be exerted when attributing predictive value to the results of a particular study. 相似文献
89.
Ivan M. Korabel Lidiia V. Panchak Alina R. Zyn Volodymyr O. Antonyuk 《Biomedical chromatography : BMC》2022,36(8):e5386
Nowadays, amaranth is a valuable multipurpose crop and a source of a number of very important biologically active substances. The aim of this study was to develop a comprehensive scheme for obtaining fatty oil, triterpenoids and lectin from the seeds of Amaranthus caudatus L. in one technological cycle. Two variants of the lectin and triterpene compound purification method from amaranth seeds were tested. It was determined that the extraction of triterpene compounds should be carried out after purification of the lectin from degreased seeds. The rationality of this sequence of technological operations is explained by the lability of the lectin and the insolubility in water of triterpene compounds from amaranth seeds. The study also presents a scheme for obtaining squalene from amaranth oil by chromatography on silica gel and proposes a more effective affinity sorbent for purification of the lectin. The use of such a sorbent also opens up the possibility of preserving other water-soluble substances from amaranth seeds. The physicochemical characteristics and carbohydrate specificity of the lectin are described and new data on the results of the interaction of lectin with human and animal erythrocytes are given. The obtained results are discussed in the light of the complex use of raw materials. 相似文献
90.
Mehdi Khoobdel Vahid Rahimi Asgar Ebadollahi Patcharin Krutmuang 《Molecules (Basel, Switzerland)》2022,27(3)
Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), is one of the most destructive stored-product pests that is resistant to a wide range of chemical insecticides. In the present study, we investigated whether a lectin extracted from Polygonum persicaria L. (PPA) can be used as a biorational agent to control such insect pests. Along with the lethal digestive assay, the sub-lethal insecticidal activities of PPA, including the effects on digestive, detoxifying, and antioxidant enzyme activities, were evaluated against S. oryzae adults. The effect of feeding a diet containing PPA and carob extract as a food attractant on the mortality of S. oryzae adults was also investigated. Feeding on the diet containing PPA resulted in a significant mortality of S. oryzae adults with a LC50 (Lethal Concentration to kill 50% of insects) of 3.68% (w/w). The activity of digestive enzymes, including α-amylase, α-glucosidase, TAG-lipase, trypsin, chymotrypsin, elastase, and carboxy- and aminopeptidase, were decreased by the sub-lethal concentration of PPA. Detoxifying and antioxidant enzymes, including esterase, superoxide dismutase, catalase, glutathione-S-transferase, ascorbate peroxidase, glucose 6-phosphate dehydrogenase, and malondialdehyde, were activated in adults affected by PPA. These findings indicated that PPA, in addition to causing digestive disorders, leads to oxidative stress in S. oryzae. The presence of carob extract had no effect on the PPA-induced mortality of the insect. According to the results of the present study, PPA has promising insecticidal efficiency against S. oryzae. In addition, the usage of PPA with a food attractant carob extract in bait traps can be recommended as a new biorational formulation in S. oryzae management. 相似文献