Removal of envelope protein-free retroviral vectors by anion-exchange chromatography to improve product quality

We have investigated the role of the retroviral lipid bilayer and envelope proteins in the adsorption of retroviral vectors (RVs) to a Fractogel DEAE matrix. Intact RVs and their degradation components (envelope protein-free vectors and solubilized vector components) were adsorbed to this matrix and eluted using a linear gradient. Envelope protein-free RVs (Env(-)) and soluble envelope proteins (gp70) eluted in a significantly lower range of conductivities than intact RVs (Env(+)) (13.7-30 mS/cm for Env(-) and gp70 proteins vs. 47-80 mS/cm for Env(+)). The zeta (zeta)-potential of Env(+) and Env(-) vectors was evaluated showing that envelope proteins define the pI of the viral particles (pI (Env(+)) < 2 versus 3 < pI (Env(-)) < 4) and that Env(+) and Env(-) vectors have similar zeta-potentials within pH 5 and 8. The results presented herein indicate that the adsorption of retroviral particles occurs through multi-point interaction of the envelope proteins with the cationic groups on the chromatographic matrix. The strength of this adsorption is thus dependent on the amount of envelope protein present in the viral lipid bilayer. In conclusion, AEXc enables the separation of gp70 proteins as well as envelope protein-free vectors constituting a significant improvement to the quality of retroviral preparations for gene therapy applications.     

Conditional limiting distribution of beta-independent random vectors

The paper deals with random vectors in , possessing the stochastic representation , where R is a positive random radius independent of the random vector and is a non-singular matrix. If is uniformly distributed on the unit sphere of , then for any integer m<d we have the stochastic representations and , with W≥0, such that W² is a beta distributed random variable with parameters m/2,(d−m)/2 and (U₁,…,U_m),(U_m+1,…,U_d) are independent uniformly distributed on the unit spheres of and , respectively. Assuming a more general stochastic representation for in this paper we introduce the class of beta-independent random vectors. For this new class we derive several conditional limiting results assuming that R has a distribution function in the max-domain of attraction of a univariate extreme value distribution function. We provide two applications concerning the Kotz approximation of the conditional distributions and the tail asymptotic behaviour of beta-independent bivariate random vectors.     

Geometric quantization, reduction and decomposition of group representations

We consider a Hamiltonian action of a connected group G on a symplectic manifold (P, ω) with an equivariant momentum map and its quantization in terms of a K?hler polarization which gives rise to a unitary representation of G on a Hilbert space . If O is a co-adjoint orbit of G quantizable with respect to a K?hler polarization, we describe geometric quantization of algebraic reduction of J ⁻¹(O). We show that the space of normalizable states of quantization of algebraic reduction of J ⁻¹(O) gives rise to a projection operator onto a closed subspace of on which is unitarily equivalent to a multiple of the irreducible unitary representation of G corresponding to O. This is a generalization of the results of Guillemin and Sternberg obtained under the assumptions that G and P are compact and that the action of G on P is free. None of these assumptions are needed here. Dedicated to Vladimir Igorevich Arnold     

碎纸片的拼接复原方法研究

本文研究了来自同一页印刷品的中、英文文字文件,在规则的横、纵切割方式下,碎纸片的拼接复原方法.文中利用碎纸片图片边缘灰度值数据的匹配度和行间距、字高等文字排版数据,建立了一系列拼接复原模型.首先对仅纵切方式下的碎纸片拼接问题,建立了基于碎片边缘匹配的图像拼接复原模型,根据碎片边缘灰度值向量间的距离来衡量两张图片的匹配度.然后对中、英文碎纸片分别提出基于像素点投影和灰度计数的分类方法,将处在同一行的碎片分为一类,使用拼接复原模型完成行内的拼接.最后通过行间距信息完成了行与行之间的匹配,从而完成了整份文件的拼接复原.     

Analysis of Off-Axial Optical Systems (1)

We have established a new method of aberration analysis for off-axial optical systems which are generalized concepts of co-axial optical systems, by introducing two kinds of newly defined 4-element vectors and expanding these vectors with the help of tensor algebra. In this method, since aberration properties are represented in tensor form, we can easily formulate the aberration relations between different azimuths. We can then evaluate the azimuth dependence of aberration properties in greater detail by separating them into inherent optical properties parts, which are independent of azimuths, and the paraxial ray-tracing part, which includes the expression of the evaluation azimuth.     

用于昆虫外激素研究的微量顶空收集及热解吸气相色谱进样方法初探

描述了一个结合项空气流收集与无溶剂热解吸气相色谱进样的方法。用填充PorapskQ的微量注射器作为吸附管进行气流收集。将收集物不经溶剂洗脱直接进行热解吸进样。用人工合成的昆虫外激素化合物反-7-十二碳单烯乙酸酯（E-7-DA）及顺-5,反-7-十二碳二烯乙酸酯（Z-5,E-7-DDA）测定了方法的回收率,初步探索了运用于昆虫外激素分析的可行性,并讨论了提高回收率的途径。     

On Existence and Weak Stability of Matrix Refinable Functions

We consider the existence of distributional (or L ₂ ) solutions of the matrix refinement equation where P is an r×r matrix with trigonometric polynomial entries. One of the main results of this paper is that the above matrix refinement equation has a compactly supported distributional solution if and only if the matrix P (0) has an eigenvalue of the form 2 ⁿ , . A characterization of the existence of L ₂ -solutions of the above matrix refinement equation in terms of the mask is also given. A concept of L ₂ -weak stability of a (finite) sequence of function vectors is introduced. In the case when the function vectors are solutions of a matrix refinement equation, we characterize this weak stability in terms of the mask. August 1, 1996. Date revised: July 28, 1997. Date accepted: August 12, 1997.     

利用有限域上非零向量的道路图结构与内积构作结合方案

Um（n,R）表示域F上n元非零向量之集．本文先确定了有限域上n≥5维非零向量的道路图结构,然后利用有限域Fq上Um（n,R）／EnR中具有相同范数的向量的道路图结构与内积构作了具有多个结合的结合方案,并计算出相应的参数．     

捷联惯导系统静基座解析粗对准的误差研究

本文讨论了两种陆用捷联惯导系统（ＳＤＩＮＳ） 在静基座情况下解析粗对准的计算方法,求解载体到导航坐标系的变换矩阵时,选择不同的参考矢量将导致失准角不同。计算和仿真结果对比给出了形象的说明。     

农作物冠层光谱信息检测技术及方法综述

相比传统的化学方法及主观视觉测定植物生理信息指标,通过光谱辐射仪对农作物冠层信息的评估更简单、快速和精确。本文结合近年来农作物群体信息的获取方法,综述了不同类型光谱仪尤其以国际上应用最广泛的Cropscan多光谱辐射仪在农作物群体叶面积指数、生物量、氮素及叶绿素的预测,病虫害的监测及产量预测中的应用。总结了不同类型的的植被指数(VIs)、冠层光谱的获取及光谱分析方法,比较了不同农作物建立相关模型的回归系数。总体上建立的数学模型的相关系数较高,能实现对农作物各种生理信息等的检测。此外,将多光谱辐射仪与多种传感器相结合所得到的综合信息对于全面评价农作物生长情况具有重要指导价值。