MALDI－TOF－MS法分析人血清白蛋白

用基质辅助激光解吸附飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法分析测定了人血清白蛋白。结果表明，全军血液制品研究中心应用Ｒｉｖａｎｏｌ和Ｃｏｈｎ＇ｓ两种方法分离提取的人血清白蛋白，其分子量与理论计算值相吻合，其纯度与市售标准人血清白蛋白相一致，用ＭＡＬＤＩ－ＴＯＦ－ＭＳ法分析鉴定人血清白蛋白，准确、简便和灵敏（仅需ＰＭ浓度样品），且重复性好，日内和日间差的变异系数均小于０．０３％，是ＳＤＳ－ＰＡＧＥ电泳法所无法比拟的。     

Human insulin and desamido human insulin isotherms in ethanol-water reversed phase systems

The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C18) and with 29.8% (w/w) ethanol-water as mobile phase have been determined experimentally. The isotherms of HI in ethanol-water differ from those obtained with the more commonly applied methanol-water and acetonitrile-water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: qHI = (8.4C(HI) + 3C(HI)CdHI)/(1 - 0.05C(HI) - 0.14CdHI + 0.04C(HI)CdHI) and qdHI = (11.4CdHI + 2C(HI)CdHI)/ (1 - 0.05C(HI) - 0.14CdHI + 0.04C(HI)CdHI)     

环境样品中痕量邻苯二甲酸酯的分离与测定

本文研究了以小粒径（１０－４０μ）硅胶柱色谱分离富集、反相高效相色谱分析测定大气颗粒物、土主植物样品中痕量邻苯二甲酸酯的方法，方法操作简单，回收率高，空白值低，利用该方法分析了部分环境样品中邻苯二甲酯酯含量。     

The ICP-MS approach to environmental studies

The certification of marine materials for trace metals is a process which challenges every technique involved, especially if a technique is as recent as inductively coupled plasma mass spectrometry (ICP-MS), Developmental work was required for several materials (natural waters, biological materials, marine sediments). It is reviewed here, in an attempt to show how one can take full advantage of ICP-MS. This includes a review of the digestion procedures developed for the multielement analysis of biological materials and marine sediments in order to minimize spectroscopic interferences. The multielement analysis of natural waters is also reviewed, in particular that of saline waters which requires a separation of the analytes from the alkali and alkaline earths elements and a preconcentration of the analytes on a column of silicaimmobilized 8-hydroxyquinoline. The potential of performing this separation/preconcentration procedure on-line is showed using both published and original results. Finally, the application of ICP-MS to speciation is illustrated by the determination of methylmercury in biological materials after extraction, and by the determination of arsenic species by high performance liquid chromatography coupled to ICP-MS.Presented in part at the 1989 European Winter Conference on Plasma Spectrochemistry, Reutte, AustriaThe work described was carried out while the author was a Research Associate at the Analytical Chemistry Section, Chemistry Division, National Research Council of Canada, Ottawa, K1A OR9, Canada     

光度法测定水样中铝

研究了铝与对乙酰基偶氮胂(ASApA)显色反应,在pH 4.7的HOAc-NaOAc缓冲溶液中,Al3+与ASApA显色形成1:2络合物,最大吸收波长位于612 nm,ε-8.19×103L·mol-1·cm-1,铝量在0～2.8μg·ml-1范围内遵守比耳定律,用于水样中铝的测定,结果满意。     

Determination of Organochlorine Pesticides in Sediments from Authie Bay Using Microwave Assisted Solvent Extraction

The aim of this study was to develop an analytical method to determine the organochlorine concentrations in sediments. Combination of Microwave assisted Solvent Extraction (MASE) and capillary gas chromatography with specific detection (electron capture detector) was a viable approach for the determination of pesticides in solid matrixes. In this study, MASE development was focused on the selection of a suitable extraction solvent for all the target analytes. MASE procedure was validated by comparison with conventional methods such as Soxhlet and sonication extraction. The proposed method was then applied to determine the organochlorine insecticides concentrations in samples from Authie Bay (France). Environmental water samples were analysed and five target analytes were detected in concentrations from 0.03 to 0.56 ng/g of dry sediment. These investigations showed the accumulation and the persistency of these products in sediments in spite of the fact they were banned a few decades ago.     

Determination of uranium in solution at the ng 1−1 level by time-resolved laser-induced spectrofluorimetry: application to geological samples

Time-resolved laser-induced spectrofluorimetry was used for the determination of uranium at the ng 1^?1 level in alkaline water with high chloride and sulphide contents in order to obtain a better understanding of uranium mobilization under natural reducing conditions. The optimum complexing medium, limit of detection and results obtained for several waters from different localities in the Pyrenees are presented. These data seem to confirm that even at very low uranium concentrations, thermodynamic control by a mineral phase takes place.     

鲁米诺-双氧水-铬(Ⅲ)化学发光体系测定化探样品中痕量砷

用鲁米诺－双氧水－铬（Ⅲ）化学发光体系结合巯基棉对Ａｓ（Ⅲ）的富集分离，建立了快速的测定化探样品中痕量砷的新方法，检测线性范围为１．０×１０－４～１．０×１０１ｍｇ／Ｌ，检出限为３．４×１０－５ｍｇ／Ｌ。用于实际化探样品测定，相对标准偏差为５．５％～９．０％。     

微量元素对人体健康的影响

阐述了微量元素的生理作用以及对人体健康的影响和微量元素平衡的重要性，对临床医学及日常生活有一定的参考价值。     

Determination of L-Dopa and L-Dopamine in Aqueous Solutions Using In-Loop SPME Coupled with LC

A new in-loop solid-phase microextraction (in-loop-SPME) technique, based on an aluminum capillary tube coupled to HPLC, is described for on-line isolation, concentration, and analysis of analytes from aqueous samples. L-Dopa and L-dopamine, in aqueous solutions, were selected as model compounds. The main conditions affecting extraction of the analytes from aqueous samples, desorption, injection, and chromatographic separation were investigated. The method is simple and reproducible. Using the proposed method, reliable determination of L-dopa and L-dopamine at parts-per-billion concentrations was achieved. The calibration plots were linear in the range of 2.5–1500 ng mL⁻¹ with correlation coefficients of 0.999 and 0.998 for L-dopa and L-dopamine, respectively. The detection limits were 0.5–1 ng mL⁻¹ with a relative standard deviation less than 4.1%. Concentration factors more than 100-fold were obtained for these compounds.