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71.
This essay discusses some preliminary thoughts on the development of a rational and modular approach for molecular design in soft matter engineering and proposes ideas of structural and functional synthons for advanced functional materials. It echoes the Materials Genome Initiative by practicing a tentative retro-functional analysis(RFA) scheme. The importance of hierarchical structures in transferring and amplifying molecular functions into macroscopic properties is recognized and emphasized. According to the role of molecular segments in final materials, there are two types of building blocks: structural synthon and functional synthon. Guided by a specific structure for a desired function, these synthons can be modularly combined in various ways to construct molecular scaffolds. Detailed molecular structures are then deduced, designed and synthesized precisely and modularly. While the assembled structure and property may deviate from the original design, the study may allow further refinement of the molecular design toward the target function. The strategy has been used in the development of soft fullerene materials and other giant molecules. There are a few aspects that are not yet well addressed:(1) function and structure are not fully decoupled and(2) the assembled hierarchical structures are sensitive to secondary interactions and molecular geometries across different length scales. Nevertheless, the RFA approach provides a starting point and an alternative thinking pathway by provoking creativity with considerations from both chemistry and physics. This is particularly useful for engineering soft matters with supramolecular lattice formation, as in giant molecules, where the synthons are relatively independent of each other. 相似文献
72.
Yu Zhang Xinyu Ling Xiaoxuan Su Shilin Zhang Dr. Jing Wang Dr. Pingjing Zhang Dr. Wenjian Feng Dr. York Yuanyuan Zhu Prof. Dr. Tao Liu Prof. Dr. Xinjing Tang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(47):21081-21085
Currently CRISPR/Cas9 is a widely used efficient tool for gene editing. Precise control over the CRISPR/Cas9 system with high temporal and spatial resolution is essential for studying gene regulation and editing. Here, we synthesized a novel light-controlled crRNA by coupling vitamin E and a photolabile linker at the 5′ terminus to inactivate the CRISPR/Cas9 system. The vitamin E modification did not affect ribonucleoprotein (RNP) formation of Cas9/crRNA/tracrRNA complexes but did inhibit the association of RNP with the target DNA. Upon light irradiation, vitamin E-caged crRNA was successfully activated to achieve light-induced genome editing of vascular endothelial cell-growth factor A (VEGFA) in human cells through a T7E1 assay and Sanger sequencing as well as gene knockdown of EGFP expression in EGFP stably expressing cells. This new caging strategy for crRNA could provide new methods for spatiotemporal photoregulation of CRISPR/Cas9-mediated gene editing. 相似文献
73.
Wentian Li 《Complexity》2012,17(4):49-53
A previous discussion of a linguistic law called Menzerath's law (the longer a word, the shorter the syllables) in the genomic context was focused on the genome‐chromosome‐base level (the more number of chromosomes in a genome, the smaller the chromosome size). We apply this linguistic metaphor to more appropriate levels of gene, exon, and base. Using the human gene data, we found that the Menzerath's law at these levels holds true: the more number of exons in a gene, the shorted the averaged exon size. Since this negative correlation can be a trivial consequence of the constant size of the messenger RNA coded by the gene, we also exclude this possibility by showing that messenger RNA size increases with the number of exons. This increase of messenger RNA size is however not fast enough for genes with large number of exons to maintain a constant exon size. © 2011 Wiley Periodicals, Inc. Complexity, 2011. 相似文献
74.
Difference spectra based on the magnitude of the quadrupolar coupling of a site has been observed under static conditions utilizing a double frequency sweep pulse sequence to enhance the central transition of a small electric field gradient (EFG) environment. Through the use of convergent sweeps that only cover the inner satellite transitions of the smaller EFG site, an echo spectrum results that favors the smaller site, which can then be used in conjunction with normal echo spectra to create a difference spectrum that consists primarily of the smaller site. The simplification of the static lineshape data permits simulation for the extraction of chemical shift anisotropy (CSA) information for the site. The method is demonstrated using 93Nb NMR for samples with multiple niobium environments due to mixtures of compounds, MgNb2O6/LiNbO3, or due to crystallographic structure, KCa2Nb3O10. 相似文献
75.
提出一种新的基于多元线性回归计算的无畸变极化转移增益法(DEPT)谱编辑方法.该方法使用多元线性回归对谱图中的杂信号进行拟合,获得最佳拟合系数,然后将拟合系数代入拟合公式,通过该公式对DEPT 子谱进行计算,去除杂信号,最终分别得到纯净的CH3、CH2、CH 子谱图.实验结果证明,使用新方法对DEPT 谱进行编辑能够很好地减少杂信号的干扰,得到只含有CHn 信号的子谱.新方法为碳谱的解析尤其是复杂和密集的谱线的解析提供了方便. 相似文献
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Pantoja-Uceda D Santoro J 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2008,195(2):187-195
In this work, we introduce a set of pulse sequences that provide amino acid type identification of the NH correlation signals of proteins. The first pulse sequence is a modification of the CBCA(CO)NH experiment that exploits spin-coupling topologies to differentiate between amino acid types. A set of eight 2D 1H–15N correlation spectra is recorded where the sign of the cross-peaks change from one spectrum to another according to the amino acid type of the preceding residue in the protein sequence. Linear combination of these eight data sets produces four subspectra. Taking also into account the sign of the correlation signals, this method allows the classification of the NH signals into six different groups, depending on the character of the preceding residue. This sequence is complemented with a (CGCBCACO)NH experiment that allows the subdivision of the largest of these groups into two smaller ones. Finally, a modification of the CBCANH experiment led to a similar classification of NH signals into six different groups, but now depending on the type of its own amino acid. The set of pulse sequences is demonstrated with two proteins of small to moderate size. 相似文献
78.
Romen Singh Naorem Bandana Devi Pangabam Sudipta Sankar Bora Gunajit Goswami Madhumita Barooah Dibya Jyoti Hazarika Csaba Fekete 《Molecules (Basel, Switzerland)》2022,27(7)
Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen and responsible for causing life-threatening infections. The emergence of hypervirulent and multidrug-resistant (MDR) S. aureus strains led to challenging issues in antibiotic therapy. Consequently, the morbidity and mortality rates caused by S. aureus infections have a substantial impact on health concerns. The current worldwide prevalence of MRSA infections highlights the need for long-lasting preventive measures and strategies. Unfortunately, effective measures are limited. In this study, we focus on the identification of vaccine candidates and drug target proteins against the 16 strains of MRSA using reverse vaccinology and subtractive genomics approaches. Using the reverse vaccinology approach, 4 putative antigenic proteins were identified; among these, PrsA and EssA proteins were found to be more promising vaccine candidates. We applied a molecular docking approach of selected 8 drug target proteins with the drug-like molecules, revealing that the ZINC4235426 as potential drug molecule with favorable interactions with the target active site residues of 5 drug target proteins viz., biotin protein ligase, HPr kinase/phosphorylase, thymidylate kinase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, and pantothenate synthetase. Thus, the identified proteins can be used for further rational drug or vaccine design to identify novel therapeutic agents for the treatment of multidrug-resistant staphylococcal infection. 相似文献
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