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Electrokinetic gating, functioning as a micro-valve, has been widely employed in microfluidic chips for sample injection and flow switch. Investigating its valving performance is fundamentally vital for microfluidics and microfluidics-based chemical analysis. In this paper, electrokinetic gating valve in microchannels was evaluated using optical imaging technique. Microflow profiles at channels junction were examined, revealing that molecular diffusion played a significant role in the valving disable; which could cause analyte leakage in sample injection. Due to diffusion, the analyte crossed the interface of the analyte flow and gating flow, and then formed a cometic tail-like diffusion area at channels junction. From theoretical calculation and some experimental evidences, the size of the area was related to the diffusion coefficient and the velocity of analytes. Additionally, molecular diffusion was also believed to be another reason of sampling bias in gated injection. 相似文献
84.
Bixue Hou Guofu Chen Zhiqi Hao Shan Feng Shuyan Wang Yishan Wang Guozhi Wang 《中国科学A辑(英文版)》1999,42(8):852-858
The experimental results of imaging through flesh tissue using fs electronic holographic gating method is reported. In the
experiment, Ti: sapphire mode-locked laser is used as light source, of which the repetition rate is 100 MHz, central wavelength
800 nm, duration of pulse 20 fs, output power 80 mW. Tissue is a 7 mm thick chicken slice, and the imaged object is a metal
wire with diameter of 0.5 mm. A general CCD is used to record holograms and a clear image of metal wire is obtained. Several
relevant problems are discussed. 相似文献
85.
针对于多目标在机载多普勒盲区测量数据丢失下的跟踪问题,提出了一种鲁棒无偏转换自适应门限的CPHD(Robust Unbiased Converted Measurements-Adaptive Gating-Cardinalized Probability Hypothesis Density,RUCM-AG-CPHD)算法。该算法首先对目标测量信息进行无偏转换,并将无偏转换得到的噪声协方差矩阵做解耦;然后设计增益调节矩阵提高滤波器在目标量测数据丢失下的鲁棒性;最后采用自适应门限去除不相关的量测信息,同时保证检测到新出现的目标,从而有效地降低了算法的计算复杂度。仿真结果表明该算法的有效性和可行性,可以更加准确的估计出目标在盲区内测量信息丢失下的目标个数和状态,且计算量相对于传统的CPHD算法减少了8.6%。 相似文献
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87.
June T. Ruszkowski Raymond Damadian Anthony Giambalvo Antoinette Gomes David Hertz Robert Lufkin S. David Smith Donald Wortham 《Magnetic resonance imaging》1986,4(6):497-502
Spin echo images of the carotid arteries in longitudinal view have been obtained by selection of oblique imaging planes. Blood flow within the lumen in the region of the carotid bifurcation has been visualized through the use of cardiac gating during end diastole. Using a surface coil placed about the mandible, high resolution images [(0.75 mm)2 per pixel) were obtained with scan times typically equal to 9 min and image data matrix equal to 256 × 256. Images obtained with this technique of MRI carotid angiography demonstrate blood flow phenomenon as well as vascular anatomy. 相似文献
88.
Guido Buonincontri Carmen Methner Thomas Krieg T. Adrian Carpenter Stephen J. Sawiak 《Magnetic resonance imaging》2014
Radial acquisitions can suffer from trajectory errors leading to reduced image quality. Here we present a new method of trajectory correction that uses all spokes of a radial acquisition and compare it to an existing method that uses a two-spoke pre-scan calibration. For both methods, estimates of the necessary shifts were made using magnitude or phase data and the performances were compared. The additional effect of B0 correction was considered in all cases. Mouse cardiac scans were used for the comparisons and we also compared the quality of navigator signals obtained from the radial data with each technique. 相似文献
89.
Water confined in nanoscale space behaves quite differently from that in the bulk.For example,in biological aquaporins and in carbon nanotubes,the traversing water molecules form a single file configuration.Water would stay in vapor state in extremely hydrophobic narrow nanopores owing to the physicochemical interactions between the water molecules and the surface of the nanopore.A spontaneous wet-dry transition has been identified in both biological and artificial nanopores.The nanopore is either fulfilled with liquid water or completely empty.Based on this mechanism,the wetting and dewetting processes inside nanopores have been further developed into highly efficient nanofluidic gates that can be switched by external stimuli,such as light irradiation,electric potential,temperature,and mechanical pressure.This review briefly covers the recent progress in the special wettability in nanoconfined environment,water transportation through biological or artificial nanochannels,as well as the smart nanofluidic gating system controlled by the water wettability. 相似文献
90.
Data acquisition and analysis of the time-resolved fluorescence anisotropy is typically a time consuming process preventing usage of this experimental method for monitoring of time-dependent phenomena. We describe a method for pseudo real-time monitoring of the limiting fluorescence anisotropy r(infinity) allowing to track changes of the membrane order occurring on the time scale of minutes. Principle and performance of the method is demonstrated in the time domain with the time-correlated single photon counting detection. DMPC liposomes stained with 1,6-diphenyl-1,3,5-hexatriene (DPH) have been used to test influence of the diffusion membrane potential on the membrane order during the temperature-induced phase transition in DMPC membranes. It has been found that the transmembrane field of the order of -70 mV increases the phase transition temperature by about 1.5 degrees C-2 degrees C. It is proposed that the full advantage of the method can be utilized with a gated detection, which besides a faster data acquisition brings additional advantage of excitation light suppression. The method can be also used for imaging. 相似文献