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181.
Bushra Asad Taimoor Khan Faiza Zareen Gul Muhammad Asad Ullah Samantha Drouet Sara Mikac Laurine Garros Manon Ferrier Shankhamala Bose Thibaut Munsch Duangjai Tungmunnithum Arnaud Lanoue Nathalie Giglioli-Guivarch Christophe Hano Bilal Haider Abbasi 《Molecules (Basel, Switzerland)》2021,26(15)
In vitro cultures of scarlet flax (Linum grandiflorum L.), an important ornamental flax, have been established as a new possible valuable resource of lignans and neolignans for antioxidant and anti-inflammatory applications. The callogenic potential at different concentrations of α-naphthalene acetic acid (NAA) and thidiazuron (TDZ), alone or in combinations, was evaluated using both L. grandiflorum hypocotyl and cotyledon explants. A higher callus induction frequency was observed on NAA than TDZ, especially for hypocotyl explants, with a maximum frequency (i.e., 95.2%) on 1.0 mg/L of NAA. The presence of NAA (1.0 mg/L) in conjunction with TDZ tended to increase the frequency of callogenesis relative to TDZ alone, but never reached the values observed with NAA alone, thereby indicating the lack of synergy between these two plant growth regulators (PGRs). Similarly, in terms of biomass, NAA was more effective than TDZ, with a maximum accumulation of biomass registered for medium supplemented with 1.0 mg/L of NAA using hypocotyls as initial explants (DW: 13.1 g). However, for biomass, a synergy between the two PGRs was observed, particularly for cotyledon-derived explants and for the lowest concentrations of TDZ. The influence of these two PGRs on callogenesis and biomass is discussed. The HPLC analysis confirmed the presence of lignans (secoisolariciresinol (SECO) and lariciresinol (LARI) and neolignan (dehydrodiconiferyl alcohol [DCA]) naturally accumulated in their glycoside forms. Furthermore, the antioxidant activities performed for both hypocotyl- and cotyledon-derived cultures were also found maximal (DPPH: 89.5%, FRAP 866: µM TEAC, ABTS: 456 µM TEAC) in hypocotyl-derived callus cultures as compared with callus obtained from cotyledon explants. Moreover, the anti-inflammatory activities revealed high inhibition (COX-1: 47.4% and COX-2: 51.1%) for extract of hypocotyl-derived callus cultures at 2.5 mg/L TDZ. The anti-inflammatory action against COX-1 and COX-2 was supported by the IC50 values. This report provides a viable approach for enhanced biomass accumulation and efficient production of (neo)lignans in L. grandiflorum callus cultures. 相似文献
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183.
Four potential metabolites of the potassium-competitive acid blocker BYK 405879 (1) were synthesized which might be formed in vivo by enzymatic oxidation of the pyran moiety or the methyl groups attached to the (hetero) aromatic system. In all cases, the oxidation of the parent compound 1 was accompanied by a significant loss of pharmacological activity and by a decrease in lipophilicity. The target compounds 6, 14, 20, and 21 constitute valuable tool substances for the investigation of the metabolic fate of BYK 405879 (1). 相似文献
184.
The new, rapid and sensitive method for the determination of paracetamol (PAR) and its glucuronide (PG) and sulfate (PS) metabolites is proposed. The electrochemical properties of the compounds were examined by cyclic voltammetry (CV) on glassy carbon electrode (GCE). All measurements were carried out in Britton–Robinson buffers (BR) with different pH values over the pH range 1.81–7.24. The preliminary research indicated that PAR could be determined simultaneously with one of its metabolites. The linearity of calibration curves was obtained for concentrations between 1.65×10?5 and 1.65×10?4 M for PAR, 1.53×10?5 and 1.53×10?4 M for PG and 2.17×10?5 and 1.52×10?4 M for PS. 相似文献
185.
Vogliardi S Favretto D Frison G Ferrara SD Seraglia R Traldi P 《Journal of mass spectrometry : JMS》2009,44(1):18-24
Matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry was used for the rapid detection of cocaine, benzoylecgonine and cocaethylene in hair. Different MALDI sample preparation procedures have been tested and the employment of a multi-layer 'graphite-sample-electrosprayed alpha-cyano-4-hydroxycinnamic acid (HCCA)' yielded the best results for standard solutions of the target analytes. The same approach was subsequently applied to hair samples that were known to contain cocaine, benzoylecgonine and cocaethylene, as determined by a classical GC-MS method. It was however necessary to extract hair samples by incubating them in methanol/trifluoroacetic acid for a short time (15 min) at 45 degrees C; 1 microl of the obtained supernatant was deposed on a metal surface treated with graphite, and HCCA was electrosprayed on it. This procedure successfully suppressed matrix peaks and was effective in detecting all the target analytes as their protonated species. The results obtained give further confirmation of the effectiveness of the MALDI for detecting drugs and their metabolites in complex biological matrices. The method can be useful as a fast screening procedure to detect the presence of cocaine and metabolites in hair samples. 相似文献
186.
Haiying Zhang Donglu Zhang Kenneth Ray Mingshe Zhu 《Journal of mass spectrometry : JMS》2009,44(7):999-1016
Identification of drug metabolites by liquid chromatography/mass spectrometry (LC/MS) involves metabolite detection in biological matrixes and structural characterization based on product ion spectra. Traditionally, metabolite detection is accomplished primarily on the basis of predicted molecular masses or fragmentation patterns of metabolites using triple‐quadrupole and ion trap mass spectrometers. Recently, a novel mass defect filter (MDF) technique has been developed, which enables high‐resolution mass spectrometers to be utilized for detecting both predicted and unexpected drug metabolites based on narrow, well‐defined mass defect ranges for these metabolites. This is a new approach that is completely different from, but complementary to, traditional molecular mass‐ or MS/MS fragmentation‐based LC/MS approaches. This article reviews the mass defect patterns of various classes of drug metabolites and the basic principles of the MDF approach. Examples are given on the applications of the MDF technique to the detection of stable and chemically reactive metabolites in vitro and in vivo. Advantages, limitations, and future applications are also discussed on MDF and its combinations with other data mining techniques for the detection and identification of drug metabolites. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
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188.
A fast method for the measurement of metabolites of pyrene in urine was improved by HPLC with fluorescence detector using “heart‐cut” technique. This method can quantify the total amount of pyrene metabolites corresponding to glucuronic acid and sulfate conjugates as well as free 1‐OH‐Py. The hydrolyzed biological fluid was directly injected into the chromatographic system, via a column‐switching system. Pre‐treatment and analysis were performed within 0.5 and 9 min, respectively. Enzymatic hydrolysis has been optimized to not exceed 2 h. The best response function, in the 0.2–10 ng/mL range, is the linear regression, bringing simplicity, good accuracy, and low LOQ. The intra‐ and interday precision values were inferior to 1 and 2%, respectively. The proposed method provided a simple, convenient, and practical procedure to determine the level of the main urinary pyrene metabolites in biological samples. 相似文献
189.
Sasidhar Maddula Lars Mathias Blank Andreas Schmid Jörg Ingo Baumbach 《Analytical and bioanalytical chemistry》2009,394(3):791-800
Detection and immediate quantification of microbial metabolic activities is of high interest in fields as diverse as biotechnology
and infection biology. Interestingly, the most direct signals of microbial metabolism, the evolution of volatile metabolites,
is largely ignored in the literature, and rather, metabolite concentrations in the microbial surrounding or even disruptive
methods for intracellular metabolite measurements (i.e., metabolome analysis) are favored. Here, the development of a multi
capillary column coupled ion mobility spectrometer (MCC-IMS) was described for the detection of volatile organic compounds
from microbes and the MCC-IMS was used for characterization of metabolic activity of growing Escherichia coli. The MCC-IMS chromatogram of the microbial culture off-gas of the acetone-producing E. coli strain BL21 pLB4 revealed four analytes that positively correlated with growth, which were identified as ethanol, propanone
(acetone), heptan-2-one, and nonan-2-one. The occurrence of these analytes was cross-validated by solid-phase micro-extraction
coupled with gas chromatography mass spectrometry analysis. With this information in hand, the dynamic relationship between
the E. coli biomass concentration and the metabolite concentrations in the headspace was measured. The results suggest that the metabolic
pathways of heptan-2-one and nonan-2-one synthesis are regulated independent of each other. It is shown that the MCC-IMS in-line
off-gas analysis is a simple method for real-time detection of microbial metabolic activity and discussed its potential for
application in metabolic engineering, bioprocess control, and health care. 相似文献
190.
Adsorption properties of the clay mineral montmorillonite in the potassium homoionic form (KMT) was investigated to achieve the extraction and concentration of the herbicides atrazine (AT) and propazine (PROP), as well as the main degradation products of atrazine, namely deethylatrazine (DEA), deisopropylatrazine (DIA) and hydroxyatrazine (ATOH). A batch approach was proposed, with recovery percentages for AT, PROP and DIA higher than 90% at concentrations of 0.50 and 2.50 μg L−1. For DEA and ATOH, however, low recoveries were obtained. For DEA, this fact can be explained by its low Kd with KMT, contrary to ATOH, which interacts strongly with the mineral surface, hindering the complete desorption and hence, generating low recovery percentages. The influence of pH, ionic strength and humic acid was studied, and a comparison with the C18 phase as SPE cartridges was carried out. Montmorillonite showed a similar performance to commercial cartridge for concentrations of AT, DEA and PROP, but better recoveries for DIA was obtained using the clay mineral. For ATOH the recovery was also higher on the clay mineral, although for this compound the most suitable SPE cartridge is constituted by cation exchange resin. After the concentration and elution steps, the 0.50 and 2.50 μg L−1 gave chromatographic peak areas that could be easily quantified with an analytical curve obtained in the concentration range between 7.5 and 100 μg L−1. The obtained concentration factors are suitable to allow the application of the method to the monitoring of triazine residues in drinking water. 相似文献