金银花的13C NMR指纹图谱研究

利用PFT-NMR 核磁共振波谱仪测定了金银花特征提取物的¹³C NMR图谱,对金银花提取物的¹³C NMR指纹图谱进行了解析,与其有效成分绿原酸进行了比较,获得了金银花提取物的特征峰,并比较了不同金银花的优劣. 结果表明不同金银花提取物(M1, M2, Z1, Z2)的¹³C NMR指纹图谱都显示出绿原酸的特征共振峰,并具有很好的重现性和高度的特征性,可以成为金银花真伪鉴别的依据;优质(M1, Z1)与劣质(劣质M1,劣质Z1)金银花¹³C NMR图谱的差异进一步说明¹³C NMR指纹图谱可作为鉴别金银花质量的依据.     

黄柏的非水毛细管电泳指纹图谱研究

采用非水毛细管电泳(NACE)法,以pH5.8的醋酸盐-甲醇为背景电解质溶液,运行电压25kV,温度20℃,检测波长210nm,正极压力(5kPa×10s)进样,建立了黄柏药材的NACE指纹图谱。以氯化两面针碱为内标,确定黄柏的NACE指纹峰为13个。测定了10个产地黄柏的NACE指纹图谱,与对照NACE指纹图谱的相似度为0.718~0.982。本方法具有较好的精密度,各指纹峰相对迁移时间的相对标准偏差(RSD)<1.5%,相对峰面积的RSD≤5%。方法可用于黄柏药材的质量控制。     

High Excimer‐State Emission of Perylene Bisimides and Recognition of Latent Fingerprints

High excimer‐state emission in the H‐type aggregate of a novel asymmetric perylene bisimide derivative, 6 , with triethyleneglycol chains and lactose functionalization was achieved in water. Furthermore, its application for enhancing the visualization of transfer latent fingerprints from glass slides to the poly(vinylidene fluoride) (PVDF) membrane was explored, which showed clear images of the latent fingerprint in daylight and under 365 nm ultraviolet illumination.     

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC‐UV‐MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim‐Pack XR‐ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile–0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R² > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines. Copyright © 2014 John Wiley & Sons, Ltd.     

Monitoring the quality consistency of Fufang Danshen Pills using micellar electrokinetic chromatography fingerprint coupled with prediction of antioxidant activity and chemometrics

A fast micellar electrokinetic chromatography fingerprint method combined with quantification was developed and validated to evaluate the quality of Fufang Danshen Pills, a traditional Chinese Medicine, which has been used in the treatment of cardiovascular system diseases, in which the tetrahedron optimization method was first used to optimize the background electrolyte solution. Subsequently, the index of the fingerprint information amount of I was performed as an excellent objective indictor to investigate the experimental conditions. In addition, a systematical quantified fingerprint method was constructed for evaluating the quality consistency of 20 batches of test samples obtained from the same drug manufacturer. The fingerprint analysis combined with quantitative determination of two components showed that the quality consistency of the test samples was quite good within the same commercial brand. Furthermore, the partial least squares model analysis was used to explore the fingerprint–efficacy relationship between active components and antioxidant activity in vitro, which can be applied for the assessment of anti‐oxidant activity of Fufang Danshen pills and provide valuable medicinal information for quality control. The result illustrated that the present study provided a reliable and reasonable method for monitoring the quality consistency of Fufang Danshen pills.     

RAPD技术在东北地区玉米品种鉴别上的应用

应用现代分子生物学RAPD技术,对吉林省主推6个玉米杂交种及其父母本的指纹图谱进行鉴别.在30个随机引物中选出扩增效果明显、特异性高的引物R1和R2并对18份材料进行基因图谱的分析,图谱差异显著、易于区分,说明RAPD技术可用于玉米品种鉴定和纯度鉴定.     

Discrimination of Polygoni Multiflori radix and Cynanchi Auriculati radix using ultra‐high performance liquid chromatography fingerprints and chemical pattern recognition

In this work, a strategy was proposed to discriminate Polygoni Multiflori Radix (PMR) and its adulterant (Cynanchi Auriculati Radix, CAR). Ultra‐high performance liquid chromatography (UHPLC) fingerprints were established to analyze samples containing PMR, CAR and mixtures simultaneously. Multivariate classification methods were applied to analyze the obtained UHPLC fingerprints, including principal component analysis (PCA), partial least square discriminant analysis (PLS‐DA), soft independent modeling of class analogy (SIMCA), support vector machine discriminant analysis (SVMDA) and counter‐propagation artificial neural network (CP‐ANN). A plot of PCA score showed that PMR and CAR samples belonged to separate clusters (PMR class and CAR class), and samples of mixtures were located near PMR or CAR classes. Analysis by PLS‐DA, SVMDA and CP‐ANN performed well for recognition and prediction in terms of PMR and CAR samples. Moreover, the PLS‐DA method performed best in the detection of adulterated samples, even if the adulterant was about 25%.     

Liquid chromatographic fingerprint of 3-methylflavone-8-carboxylic acid established for its synthesis control analysis

A high-performance liquid chromatographic method was proposed for the separation of relative impurities in industrial 3-methylflavone-8-carboxylic acid (MFCA) and its intermediate methyl 3-propionylsalicylate (MPS). Benzoic acid (BA), MPS, 6-chloro-3-methylflavone-8-carboxylic acid (MFCA-Cl) and methyl 5-chloro-3-propionylsalicylate (MPS-Cl) in MFCA, and MPS-Cl in MPS were respectively quantified by an external standard method. As results showed, the linearity of standard curves was excellent with the relative coefficients of over 0.999 for all the detected components, and the intra- and inter-day precisions of impurities determination were satisfactory with the relative standard deviation of not more than 8.0%. Under the selected experimental condition, the chromatographic fingerprints of MFCA and MPS were drawn synthetically, and the transfer of impurities in the stepwise reactions of MFCA manufacture was elucidated. The fingerprints have great potential in instructing the production of MFCA for industrial use and in conducting the conversion of relative impurities.     

Applications of Carbon Dots (CDs) in Latent Fingerprints Imaging

Carbon dots (CDs), a new member of the carbon-based material family, possess unique properties, such as high fluorescence, non-toxicity, eco-friendliness, stability and cost-effectiveness. These properties helped CDs to receive tremendous attention in various fields, namely, biological, opto-electronic, bio-imaging and energy-related applications. Although CDs are widely explored in bio-imaging and bio-sensing applications, their effectiveness in forensic science and technology is comparatively new. In this review, applications of CDs pertaining to latent FPs recovery since 2015 to 2020 is summarized comprehensively.