Effect of Steeped Black Tea (Camellia sinensis (L.) var. assamica) on Immunoglobulin Titer and Lymphocyte Proliferation in Responses to Hepatitis B Vaccine in Mice

Effect of steeped black tea (Camellia sinensis (L.) var. assamica) on immunoglobulin titer (IgM and IgG) and lymphocyte proliferation in responses to hepatitis B vaccine inBALB/c mice has been investigated. The study was divided into two stages i.e. the determination of immunoglobulin titer and the test of lymphocytes proliferation. In the first stage mice were divided into 5 groups each consisting of 5 mice. Group I, II and III was given steeped black tea respectively with a dose of 600 mg/kg bw; 1.2 g/kg bw and 2.4 g/kg bw. Group IV was given Stimuno^® with a dose of 6.5 mg/kg bw, and group V was given aquadest as negative control. All groups were induced by hepatitis B vaccine on day-0 (after 7 days of acclimatization). Serum was taken on day-14 and 21 for measurement of IgM titer and IgG, respectively. In the second stage, the mice were grouped as in the first stage, then all groups were induced by hepatitis B vaccine at day-0 and day-7. On day-27 lymphocyte was isolated and then tested for the growth and proliferation of lymphocytes. The results of this study showed that the steeped black tea has an effect in increasingIgM and IgG titer of BALB/cmice induced by hepatitis B vaccine, where the most effective dose was 1.2 g/kg bw. Steeped black tea also could increase lymphocytes proliferation in mice BALB/cinduced by hepatitis B vaccine, where the most effective dose was 1.2 g/kg bw.     

EXPRESSION AND SECRETION OF preS CONTAINING HEPATITIS B SURFACE ANTIGEN IN VACCINIA VIRUS SYSTEM

The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK~- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2+S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1+preS2+S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1.     

血液乙肝病毒基因检测

全世界每年死于乙型肝炎病毒相关疾病的人数已达60万人,应用荧光定量PCR法检测能够提高乙肝病毒基因的检出率,正确掌握该技术方法能够更加有效地进行疾病诊断和预防工作。基于此,研究针对荧光定量PCR法检测乙肝病毒基因的方法进行探讨。     

乙型肝炎疫苗预防效果探讨

目的分析研究乙型肝炎疫苗预防效果。方法选取2013年4月—2014年4月中山市三乡医院收治的220例儿童作为研究对象,220例对象全部以经过乙型肝炎疫苗接种,随机分为观察组、对照组,对照组儿童没有给予增强乙型肝炎疫苗接种,观察组儿童则在乙肝疫苗接种的基础之上给予加强乙型肝炎疫苗接种,对两组儿童乙型肝炎疫苗的预防效果进行对比。结果观察组儿童的抗-HBs阳性率和抗-HBc阳性率显著高于对照组(P0.05);观察组儿童的HBs Ag阳性率明显低于对照组(P0.05)。结论乙肝疫苗预防接种免疫人群效果良好,增强乙肝疫苗接种可以有效降低大规模疫苗接种当中不可预知因素对其预防效果的影响,进而使乙肝疫苗预防性接种效果明显提高。     

A Concise Synthesis of the HCV Protease Inhibitor BILN 2061 and Its P3 Modified Analogs

A concise synthesis of BILN 2061 was achieved through more efficient installation of P2 4‐quinoline moiety via S_N2 displacement of the β‐OBs group located on the 4‐hydroxyl proline intermediate, which was prepared from 4‐α‐hydroxyl proline analog via Mitsunobu reaction with inversion of stereochemistry. In addition, a short and practical synthesis for P3 unit is also described herein. Final assembly of four fragments for BILN 2061 was achieved with an overall yield of 58% in 4 steps from P1 to 15a . Furthermore several analogs of BILN 2061 ( WX‐1 – WX ‐ 5 ) containing modifications on P3 unit were synthesized successfully using the same synthetic route as described for the parent inhibitor BILN 2061 .     

Surface-enhanced Raman spectroscopy coupled with gold nanoparticles for rapid detection of amoxicillin residues in duck meat

Antibiotic residues in meat pose serious threats to human beings and animals, and are one of the main food safety concerns all around the world. A novel method based upon surface-enhanced Raman spectroscopy (SERS) coupled with gold nanoparticles was developed in this study for rapid detection of amoxicillin residues in duck meat, one widely used in duck farming. Three different particle sizes of gold nanoparticles were synthesized using 0.3, 0.7, and 1.1?mL 1% trisodium citrate, while 0.7?mL was found to be the optimal volume as Raman enhancement substrate for the detection of amoxicillin residues in duck meat. The amounts of gold nanoparticles (0.5?mL), duck meat extract containing amoxicillin (50?μL), and sodium chloride solution (100?μL), as well as adsorption time (5?min) were optimized by single factor experiments. A calibration curve with the determination coefficient of 0.9661 was established for quantitative analysis of unknown amoxicillin samples. The average recoveries of amoxicillin residues in duck meat extract were 96–139%. This study provided a rapid and simple approach to analyze amoxicillin residues in duck meat in excess of 0.2?mg?L^?1 using SERS coupled with gold nanoparticles, which has the potential for wide applications in the detection of the antibiotic residues in meat.     

咸宁市饮食从业人员乙型肝炎检测结果的分析

以饮食从业人员为检测对象.每人静脉采血5 mL,分离血清,应用ELISA方法进行检测.共检1 640人,HBsAg阳性96人,阳性率为5.85%(96/1640),其中HBsAg阳性血清进一步做HBeAg、抗-HBe、抗-HBc检测,阳性率分别为7.29%(7/96)、67.7%(65/96)、91.6%(88/96).其中男性722人,阳性62名,阳性率为8.59%(62/722),女性918人,阳性34名,阳性率为3.70%.HBsAg阳性率5.85%低于全国平均水平的8.08%.男性阳性率8.59%略低于我国1992年调查的11.3%,女性阳性率3.70%则明显低于我国同期调查的女性HBsAg阳性率8.23%.     

载体表达siRNA抑制HBV复制与基因表达

用载体表达siRNA的方法并通过RNA干扰达到抑制乙型肝炎病毒（HBV）的复制及其基因的表达．针对HBV的S基因选取4个靶位点，并将目的基因与荧光素酶报道基因构成快速筛选系统，从4个位点中筛选到一个有效的作用位点．用半定量RT-PCR、ELISA、Western blot和荧光定量PCR等方法对筛选到的有效位点进行了验证，结果表明，siRNA成功降解了HBVS基因的mRNA，降低了S蛋白的表达水平，有效地抑制了HBV的复制．     

ICP-AES法测定慢性乙肝患者头发中生命元素

建立了用ＩＣＰ－ＡＥＳ法同时分析头发中多种生命元素的方法,方法的相对标准偏差为１．４％￣４．９％,回收率介于９４％￣１２９％之间。测定了东莞地区１５９例在样大学生慢性乙肝患者头发样品中的Ａｌ、Ｂａ、Ｃａ、Ｃｏ、Ｃｒ、Ｃｕ、Ｆｅ、Ｍｇ、Ｍｎ、Ｎｉ、Ｐｂ、Ｓｒ、Ｚｎ等十三种生命元素的含量,并和健康人比较,初步分析了头发中生命元素含量和慢性乙型肝炎的相关性。