排序方式: 共有21条查询结果,搜索用时 15 毫秒
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Desislava I. Mantovska Miroslava K. Zhiponova Milen I. Georgiev Tsvetinka Grozdanova Dessislava Gerginova Kalina Alipieva Svetlana Simova Milena Popova Veneta M. Kapchina-Toteva Zhenya P. Yordanova 《Molecules (Basel, Switzerland)》2021,26(19)
Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L–1 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro multiplicated plants were successively acclimated in a growth chamber and a greenhouse with 92% survival. The number of plastid pigments and the total phenolics content in in vitro cultivated and ex vitro adapted plants were unchanged, and no accumulation of reactive oxygen species (ROS) was detected by staining with 3-3′-diaminobenzidine (DAB) and 2′,7′-dichlorofluorescein diacetate (DCF-DA). Nuclear Magnetic Resonance (NMR) fingerprinting allowed for the identification of the major alterations in metabolome of V. caucasica plants during the process of ex situ conservation. Iridoid glucosides such as verproside, aucubin and catalpol were characteristic for in vitro cultivated plants, while in ex vitro acclimated plants phenolic acid–protocatechuic acid and caffeic acid appeared dominant. The successful initiation of in vitro and ex vitro cultures is an alternative biotechnological approach for the preservation of V. caucasica and would allow for further studies of the biosynthetic potential of the species and the selection of lines with a high content of pharmaceutically valuable molecules and nutraceuticals. 相似文献
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N-取代苯基-N'-(1,3,4-噻二唑-2-基)脲类化合物的合成与生物活性 总被引:5,自引:1,他引:5
通过酰基叠氮化物与2-氨基噻二唑或5-氨基-2-巯基噻二唑反应,合成了10个新的N-取代苯基-N'-(1,3,4-噻二唑-2-基)脲,用核磁共振氢谱、红外光谱和元素分析确证了它们的结构,初步的生测活性试验表明,部分目标化合物2c,2d具有良好的细胞分裂素活性. 相似文献
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重瓣丝石竹试管苗玻璃化发生原因初探 总被引:8,自引:0,他引:8
本文研究了引发重瓣丝石竹 (Gypsophila paniculata L. ) 试管苗玻璃化现象发生的若干因素 .结果表明 ,外植体取材部位显著影响玻璃苗发生频率 ,重瓣丝石竹玻璃苗发生频率以中部茎段最高 , 远高于顶芽和基部茎段. 培养基内较高浓度的细胞分裂素 , 尤为高浓度B A5mg L- 1 ,是影响玻璃苗发生的主要原因 . 琼脂浓度 ,蔗糖及培养温度等对玻璃苗发生率的影响 ,依培养基中激素的状况而异.添加适量硝酸钾 ,可以完全抑制玻璃苗的发生 相似文献
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稀土元素对植物体内细胞分裂素的影响 总被引:6,自引:0,他引:6
本文采用生物鉴定法(bioassay)和高效液相色谱法(HPLE)测定施用稀土元素的作物,从植物内源激素的角度来讨论稀土元素对植物的生理作用影响,测定结果表明:施用稀土元素后,细胞分裂素(Cyto-kinin CTC)平均增长25%左右,说明稀土元素促进了细胞分裂、诱导芽的形成。 相似文献
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Kowalska M Tian F Šmehilová M Galuszka P Frébort I Napier R Dale N 《Analytica chimica acta》2011,701(2):218-223
An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N6-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol–gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface. 相似文献
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