1-乙烯基-3-甲基咪唑离子液体/分子印迹材料用于牛血红蛋白的识别

分子印迹聚合物是一类对目标分子具备特异性辨别能力的高分子吸附剂材料.运用本体聚合法,以牛血红蛋白(BHb)为模板分子,丙烯酰胺(AAM)和碘化1-乙烯基-3-甲基咪唑离子液体在交联剂、引发剂和加速剂的作用下进行聚合,制备的分子印迹材料对牛血红蛋白(BHb)具备特异性识别功能.同时,对识别条件进行了优化和讨论.     

An Assembled Nanocomplex for Improving both Therapeutic Efficiency and Treatment Depth in Photodynamic Therapy

Photodynamic therapy (PDT) shows unique selectivity and irreversible destruction toward treated tissues or cells, but still has several problems in clinical practice. One is limited therapeutic efficiency, which is attributed to hypoxia in tumor sites. Another is the limited treatment depth because traditional photosensitizes are excited by short wavelength light (<700 nm). An assembled nano‐complex system composed of oxygen donor, two‐photon absorption (TPA) species, and photosensitizer (PS) was synthesized to address both problems. The photosensitizer is excited indirectly by two‐photon laser through intraparticle FRET mechanism for improving treatment depth. The oxygen donor, hemoglobin, can supply extra oxygen into tumor location through targeting effect for enhanced PDT efficiency. The mechanism and PDT effect were verified through both in vitro and in vivo experiments. The simple system is promising to promote two‐photon PDT for clinical applications.     

A Crosslinked Nucleic Acid Nanogel for Effective siRNA Delivery and Antitumor Therapy

Functional siRNAs are employed as cross‐linkers to direct the self‐assembly of DNA‐grafted polycaprolactone (DNA‐g‐PCL) brushes to form spherical and nanosized hydrogels via nucleic acid hybridization in which small interfering RNAs (siRNAs) are fully embedded and protected for systemic delivery. Owing to the existence of multivalent mutual crosslinking events inside, the crosslinked nanogels with tunable size exhibit not only good thermostability, but also remarkable physiological stability that can resist the enzymatic degradation. As a novel siRNA delivery system with spherical nucleic acid (SNA) architecture, the crosslinked nanogels can assist the delivery of siRNAs into different cells without any transfection agents and achieve the gene silencing effectively both in vitro and in vivo, through which a significant inhibition of tumor growth is realized in the anticancer treatment.     

Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds

Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.     

修饰硅胶表面印迹牛血红蛋白及其识别性能的研究

本文选用马来酸酐修饰后的硅胶作为载体,丙烯酰胺为功能单体,N,N’-亚甲基双丙烯酰胺为交联剂,牛血红蛋白为模板分子,采用氧化还原悬浮聚合法,合成了具有选择性识别的牛血红蛋白分子印迹聚合物。并用红外光谱(IR)、扫描电子显微镜(SEM)对聚合物进行了表征,结果表明载体表面成功接枝了分子印迹聚合物薄层。同时,选择性吸附实验表明分子印迹聚合物的具有良好的识别性能,能成功的实现水溶液中牛血红蛋白的富集。     

Interaction of giant extracellular Glossoscolex paulistus hemoglobin (HbGp) with zwitterionic surfactant N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS): effects of oligomeric dissociation

The present work focuses on the interaction between the zwitterionic surfactant N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS) and the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp). Electronic optical absorption, fluorescence emission and circular dichroism spectroscopy techniques, together with Gel-filtration chromatography, were used in order to evaluate the oligomeric dissociation as well as the autoxidation of HbGp as a function of the interaction with HPS. A peculiar behavior was observed for the HPS–HbGp interaction: a complex ferric species formation equilibrium was promoted, as a consequence of the autoxidation and oligomeric dissociation processes. At pH 7.0, HPS is more effective up to 1 mM while at pH 9.0 the surfactant effect is more intense above 1 mM. Furthermore, the interaction of HPS with HbGp was clearly less intense than the interaction of this hemoglobin with cationic (CTAC) and anionic (SDS) surfactants. Probably, this lower interaction with HPS is due to two factors: (i) the lower electrostatic attraction between the HPS surfactant and the protein surface ionic sites when compared to the electrostatic interaction between HbGp and cationic and anionic surfactants, and (ii) the low cmc of HPS, which probably reduces the interaction of the surfactant in the monomeric form with the protein. The present work emphasizes the importance of the electrostatic contribution in the interaction between ionic surfactants and HbGp. Furthermore, in the whole HPS concentration range used in this study, no folding and autoxidation decrease induced by this surfactant were observed. This is quite different from the literature data on the interaction between surfactants and tetrameric hemoglobins, that supports the occurrence of this behavior for the intracellular hemoglobins at low surfactant concentration range. Spectroscopic data are discussed and compared with the literature in order to improve the understanding of hemoglobin–surfactant interaction as well as the acid isoelectric point (pI) influence of the giant extracellular hemoglobins on their structure–activity relationship.     

HbA/H2O2: an efficient biomimetic catalytic system for the oxidation of sulfides to sulfoxides

Human hemoglobin (HbA) catalyzed chemo-selective oxidation of sulfides to sulfoxides has been reported using hydrogen peroxide as an oxidant in a phosphate buffer. This biomimetic catalytic procedure was found to be simple, environmentally friendly, selective, and high yielding. Purified human oxyhemoglobin (HbA₀), crude human oxyhemoglobin, and ferryl oxyhemoglobin were used for sulfoxidation. HbA₀ and ferryl oxyhemoglobin (Fe⁺⁴O) efficiently catalyze the sulfoxidation reaction without over oxidation to the sulfone.     

异常血红蛋白酶解肽段硅胶薄板指纹图谱法的改良

本文叙述了改进的硅胶薄板指纹图谱法用于异常血红蛋白结构分析中的肽段分离工作。此法设备简单、省时省料,使用比较简陋的实验条件即可获得较好的实验结果,克服了高效液层法(HPLC)和反相高效液层法(RP—HPLC)要求设备昂贵,高压电泳法有害气体严重以及RP—HPLC的某些失活问题等     

等速电泳分析人血红蛋白

应用等速电泳（ＩＴＰ）分析了２０例健康者、１０例糖尿病患者及３例异常血红蛋白病患者的血红蛋白组分。健康者血红蛋白的ＩＴＰ分析图可分为５个部分,即ＨｂＡ_（１ａ）,ＨｂＡ_（１ｂ）＋ＨｂＡ_（１ｃ）＋ＨｂＦ,ＨｂＡ_０,ＨｂＡ_２和ＨｂＵ,并计算出各组分的均值±标准差和各组分的相对值。病理组的ＩＴＰ分析图和各组分的测定值均与健康者明显不同。     

氧钒离子作用下人血红蛋白构象变化的动态过程及2,3-二磷酸甘油酸的影响

用停流分光光度计检测在有或无DPG结合时Hb与氧钒离子作用过程中自身荧光的变化,发现无DPG的Hb自身荧光只是一个单调的荧光猝灭过程.DPG结合于Hb后的荧光变化则先为荧光猝灭,转而缓慢增加.用氧钒离子结合引起的构象变化及DPG和DPG的水解产物3PGA所起的稳定Hb四级结构作用解释了上述过程.