A cinnamoyl substituted Nile Red-based probe to detect hydrazine

Herein we discovered that a Nile Red-based probe with a cinnamoyl unit was highly selective and sensitive to N₂H₄. Hydrazinolysis by N₂H₄ would release a hydroxyl substituted Nile Red and result in remarkable fluorescence quench. Importantly, Cys/Hcy would not interrupt the N₂H₄ recognition. This is because, for this probe, the combination of the π-π conjugate system can stabilize the ethylene union, which results in the nucleophilic addition of the thiol group of Cys/Hcy becomes non-effective.     

基于功能核酸的水中汞离子荧光检测方法

二价汞离子作为一种重要的环境污染物,一直以来受到国内外广泛关注。基于T-T错配的Hg2+检测极大依赖于汞选择性寡聚核苷酸（MSO）的设计,利用SYBR Green I对目前所报道的汞离子探针进行了优化,研究了若干种MSO探针与Hg2+的结合响应,在对探针二级结构进行分析和讨论的基础上,提出最优的富T探针序列,并由此建立了一种基于SYBR Green I的水中汞离子快速、便捷的荧光检测方法。最终测得3种实际水样的加标回收率在82.8％~101.8％之间,相对标准偏差小于15%,表明该方法受环境基质的影响较小,可应用于实际水样中的汞离子检测。     

Isatindolignanoside A,a glucosidic indole-lignan conjugate from an aqueous extract of the Isatis indigotica roots

A glucosidic indole-lignan conjugate with a novel carbon skeleton, named isatindolignanoside A (1), was isolated from an aqueous extract of the Isatis indigotica roots “ban lan gen”. Its structure was determined by comprehensive analysis of spectroscopic data, enzyme hydrolysis, and electronic CD calculations. Compound 1 is active against Coxsackie virus B3 and represents the first example of natural product having a structural feature of conjugation between indole and lignan, and its plausible biosynthetic pathway is postulated.     

The Conjugate Nets, Cartan Submanifolds, and Laplace Transformations in Space Forms

In this paper we establish the geometric theory of conjugate nets, Cartan submanifolds, and Laplace transformations in sphere and pseudo-sphere spaces. The corresponding theory in cases of projective and Euclidean spaces has been established by Chern, Kamran and Tenenblat.     

Accelerated Poisson-Boltzmann calculations for static and dynamic systems

We report here an efficient implementation of the finite difference Poisson-Boltzmann solvent model based on the Modified Incomplete Cholsky Conjugate Gradient algorithm, which gives rather impressive performance for both static and dynamic systems. This is achieved by implementing the algorithm with Eisenstat's two optimizations, utilizing the electrostatic update in simulations, and applying prudent approximations, including: relaxing the convergence criterion, not updating Poisson-Boltzmann-related forces every step, and using electrostatic focusing. It is also possible to markedly accelerate the supporting routines that are used to set up the calculations and to obtain energies and forces. The resulting finite difference Poisson-Boltzmann method delivers efficiency comparable to the distance-dependent dielectric model for a system tested, HIV Protease, making it a strong candidate for solution-phase molecular dynamics simulations. Further, the finite difference method includes all intrasolute electrostatic interactions, whereas the distance dependent dielectric calculations use a 15-A cutoff. The speed of our numerical finite difference method is comparable to that of the pair-wise Generalized Born approximation to the Poisson-Boltzmann method.     

Fourier acceleration of iterative processes in disordered systems

Technical details are given on how to use Fourier acceleration with iterative processes such as relaxation and conjugate gradient methods. These methods are often used to solve large linear systems of equations, but become hopelessly slow very rapidly as the size of the set of equations to be solved increases. Fourier acceleration is a method designed to alleviate these problems and result in a very fast algorithm. The method is explained for the Jacobi relaxation and conjugate gradient methods and is applied to two models: the random resistor network and the random central-force network. In the first model, acceleration works very well; in the second, little is gained. We discuss reasons for this. We also include a discussion of stopping criteria.     

Linear-quadratic two-person zero-sum differential games,necessary and sufficient conditions: Comment

We give a simpler, easier-to-check, version of the theorem of the paper referred to, i.e., a necessary and sufficient condition for the existence of a saddle point to the linear-quadratic two-person zero-sum perfect information differential game.     

The maximal Fejér operator on real Hardy spaces

We prove that the maximal Fej'er operator is not bounded on the real Hardy spaces H ¹, which may be considered over and . We also draw corollaries for the corresponding Hardy spaces over ² and ². This revised version was published online in August 2006 with corrections to the Cover Date.