计算精馏塔理论塔板数和加料板位置的新方法（Ⅱ）

通过作平衡曲线的弦，导出了计算精馏塔理论塔板数的显函数关联式，并确定了式中常数Ｃ、Ｅ的值．该式直接将理论塔板数和操作参数关联，在参数变化较大的范围内，其计算结果与Ｓｍｏｋｅｒ方程的精确解一致．     

Synthesis of affinity column of phosphatidylinositol-3,4-diphosphate

Phosphatidylinositol polyphosphates (PIPx) are related with tyrosine kinase activation, cell proliferation and carcinogenesis. In order to investigate the action mechanism of PIPx, it is desirable to synthesize affinity column of PI-3,4-P2, which is expected to be able to isolate the binding proteins of PI-3,4-P2. Thyramine reacted with CH-Sepharose 4B giving column 13. The p-amino group of 3'-(1',2'-distearoyl-glyceryl)-1-(2-p-aminobenzyl)-3,4-di-O-phosphoryl-myo-inosityl phosphate (12) was diazotized, then diazo-coupled with column 13 to give PI-3,4-P2 affinity column 14. This PI-3,4-P2 affinity column is an effective tool to pick up binding proteins of PI-3,4-P2.     

Constraint Programming Based Column Generation for Crew Assignment

Airline crew assignment problems are large-scale optimization problems which can be adequately solved by column generation. The subproblem is typically a so-called constrained shortest path problem and solved by dynamic programming. However, complex airline regulations arising frequently in European airlines cannot be expressed entirely in this framework and limit the use of pure column generation. In this paper, we formulate the subproblem as a constraint satisfaction problem, thus gaining high expressiveness. Each airline regulation is encoded by one or several constraints. An additional constraint which encapsulates a shortest path algorithm for generating columns with negative reduced costs is introduced. This constraint reduces the search space of the subproblem significantly. Resulting domain reductions are propagated to the other constraints which additionally reduces the search space. Numerical results based on data of a large European airline are presented and demonstrate the potential of our approach.     

Capillary gas chromatography of aromatic bicyclic and tricyclic spiro ketones

Summary The separation of some aromatic bicyclic and tricyclic spiro ketones on fused silica capillary columns coated with polydimethylsiloxane, cyanopropylmethylsiloxane and poly(ethylene glycol) stationary phases was investigated. Retention indices were determined at two temperatures in order to enable understanding of the compounds’ chromatographic behaviour. The respective standard deviation values were 0.8, 0.5 and 0.3 index units. The influence of the polarity of the stationary phases on the chromatographic retention of these cyclic spiro ketones is discussed.     

Capillary gas chromatography of amino acids as their tert-butyldimethylsilyl derivatives in the analysis of serum amino acids in metabolic diseases

Reaction of amino acids with N-methyl-N-(tert-butyldimethylsilyl)trifluoroaceamide (MTbSTFA) in acetonitrile affords good yields of amino acid derivatives with excellent gas chromatographic and mass spectrometric properties. The single-step derivatization procedure is highly reproducible. The TBDMS amino acids are stable at room temperature for at least three days. Only a single peak is observed for each amino acid. The procedure allows simultaneous analysis of asparagine and glutamine together with other serum amino acids. Separation is achieved on a borosilicate glass capillary coated with OV-1. The mass spectra of the TBDMS amino acids possess characteristic diagnostic ions. These properties were used in the sensitive detection by GC-MS and SIM-GC-MS of GABA and pipecolic acid in the serum of a newborn suspected of a Zellweger-type syndrome, which could not be detected by other methods.     

Retention in gas chromatography when there is a large pressure drop in different sections of a coupled column

Methods providing control of the selectivity of a coupled column were compared. The method of vacuum barochromatography has been developed. This method allows the selectivity of a coupled column to be controlled over a broad range of the Rohrschneider scale using medium carrier-gas pressures. The possibility of identifying the components of a complex mixture based on chromatographic spectra was considered.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2941–2947, December, 1996.     

Trichloroacetic acid in the foliage of forest trees

Trichloroacetic acid (TCA) is an atmospheric oxidation product of airborne C₂-chlorocarbons and a widespread air pollutant. It was widely used as a herbicide in the fifties and sixties, in some countries, still today. It occurs in the foliage of conifers and deciduous trees of mountain forests in Germany, e. g. the Black Forest, the Northern Alps or the Erzgebirge. Quantitative determination in the ppb-range is achieved by gas chromatography using thick film capillary columns and electron-capture detection. The levels in spruce needles at two forest sites correlate with the extent of needle loss. The data are supportive of the hypothesis that TCA is causally involved in the induction of mountain forest decline.     

Screening of trihalomethanes by direct aqueous injection using electron capture detection

The disinfection of drinking water with chlorine produces carcinogenic by-products called trihalomethanes or (THM's). These compounds are formed when the chlorine reacts with the organic matter in the raw water. The four major THM's are chloroform, bromodichloromethane, dibromochromethane, and bromoform. Direct aqueous injection is used for the rapid screening THM's and other volatile organic compounds (VOC's) in water by direct on column injection of the water sample in to wide bore capillary columns, to which is attached an electron capture detector.     

Sorption of the peroxocomplex of titanium on silica gel and its analytical use

Summary Sorption of the titanium peroxocomplex on silica gel was studied in dependence on the pH and on the presence of various complexing substances, especially tartaric and citric acids. The distribution coefficients for the titanium peroxocomplex and for some other components were determined. Optimum conditions were established for the separation of small amounts of titanium from a number of accompanying cations and anions on a column and under static conditions.