健脾养胃活血化瘀法治疗慢性萎缩性胃炎临床观察

目的观察治疗慢性萎缩性胃炎时应用健脾养胃活血化瘀法的效果。方法在2014年2月—2015年2月梅州市第二中医医院收治的慢性萎缩性胃炎患者中,选取30例作为研究对象,按照就诊时间,将患者分为实验组(n=15)和参照组(n=15),参照组患者行丽珠得乐治疗,实验组行健脾养胃活血化瘀法,对两组患者的治疗效果进行观察。结果实验组患者的治疗效果明显优于参照组(P0.05),具有统计学意义。结论在慢性萎缩性胃炎临床治疗中,采用健脾养胃活血化瘀法,患者的病情得到有效控制,患者的治疗满意度得到了提升,健脾养胃活血化瘀法在临床中应用具有重要意义。     

Identification and Preliminary Structure-Activity Relationship Studies of 1,5-Dihydrobenzo[e][1,4]oxazepin-2(3H)-ones That Induce Differentiation of Acute Myeloid Leukemia Cells In Vitro

Acute myeloid leukemia (AML) is the most aggressive type of blood cancer, and there is a continued need for new treatments that are well tolerated and improve long-term survival rates in patients. Induction of differentiation has emerged as a promising alternative to conventional cytotoxic chemotherapy, but known agents lack efficacy in genetically distinct patient populations. Previously, we established a phenotypic screen to identify small molecules that could stimulate differentiation in a range of AML cell lines. Utilising this strategy, a 1,5-dihydrobenzo[e][1,4]oxazepin-2(3H)-one hit compound was identified. Herein, we report the hit validation in vitro, structure-activity relationship (SAR) studies and the pharmacokinetic profiles for selected compounds.     

Human Group IIA Phospholipase A2—Three Decades on from Its Discovery

Phospholipase A₂ (PLA₂) enzymes were first recognized as an enzyme activity class in 1961. The secreted (sPLA₂) enzymes were the first of the five major classes of human PLA₂s to be identified and now number nine catalytically-active structurally homologous proteins. The best-studied of these, group IIA sPLA₂, has a clear role in the physiological response to infection and minor injury and acts as an amplifier of pathological inflammation. The enzyme has been a target for anti-inflammatory drug development in multiple disorders where chronic inflammation is a driver of pathology since its cloning in 1989. Despite intensive effort, no clinically approved medicines targeting the enzyme activity have yet been developed. This review catalogues the major discoveries in the human group IIA sPLA₂ field, focusing on features of enzyme function that may explain this lack of success and discusses future research that may assist in realizing the potential benefit of targeting this enzyme. Functionally-selective inhibitors together with isoform-selective inhibitors are necessary to limit the apparent toxicity of previous drugs. There is also a need to define the relevance of the catalytic function of hGIIA to human inflammatory pathology relative to its recently-discovered catalysis-independent function.     

循证护理在慢性宫颈炎合并HPV感染患者治疗指导中的应用

目的探究循证护理在慢性宫颈炎合并HPV感染患者治疗指导中的应用。方法对63例慢性宫颈炎合并HPV感染患者,经循证护理,分别于6个月和12个月追踪HPV清除率。结果 63例经循证护理的慢性宫颈炎合并HPV感染患者,6个月后和12个月后的HPV清除率分别为41.3%(26/63)和58.7%(37/63);42例对照组6个月后和12个月后的HPV清除率分别为28.6%(12/42)和58.7%(17/42)。结论对慢性宫颈炎合并HPV感染患者进行循证护理,缩短了宫颈炎治愈时间并提高HPV清除率。     

Investigation into omacetaxine solution stability for in vitro study

Omacetaxine is a natural product extract originating from Chinese medicine and finding therapeutic use as a potent myelosuppressive agent in leukemia. When planning in vitro cell biology experiments to assess omacetaxine activity against primary leukemic stem cells, it became apparent that the literature rarely describes the in vitro stability of the molecule, although accessible chromatographic methods have been published. Clearly whole organisms vs their component cells will differ in the way in which they handle xenobiotics, with the latter more dependent on physiochemical parameters such as pH and temperature in the absence of active metabolism or excretion. This could impact on the cells' experience of drug in culture. We therefore report here on examination of a modified, high‐performance liquid chromatography (HPLC) method with assessment of degradant production from a 72 h solution stability study, clearly demonstrating that omacetaxine is highly stable in representative cell culture conditions (37 °C, neutral pH) and persists for many days in marked contrast to its short‐half life in vivo. Copyright © 2011 John Wiley & Sons, Ltd.     

基于SPSS的教职工健康体检数据分析研究——以宁波大学为例

为尽早预防疾病, 开展健康管理, 改善教职工的健康现状, 对2018—2021年期间宁波大学教职工的健康体检资料采用SPSS 23.0软件进行统计分析. 结果表明 2018—2021年检出前10种疾病略有不同, 4年间检出率均位于前10位的疾病有甲状腺结节、肺结节、血脂异常、高尿酸血症、心电图异常、乳腺异常. 其中每年血脂异常、肺结节及高尿酸血症检出率男性高于女性(P< 0.05), 甲状腺结节的检出率则是女性高于男性(P<0.05); 甲状腺结节、肺结节和高尿酸血症的检出率呈现逐年升高趋势(P<0.05); 每年检出的疾病随年龄变化的态势各不相同, 其中血脂异常、甲状腺结节、肺结节、心电图异常、前列腺异常的检出率随着年龄的增长而增加(P<0.05). 表明慢性非传染性疾病已成为影响教职工健康的首要危险因素. 因此, 应倡导科学的生活方式, 积极进行健康体检, 建立健康管理档案, 并进一步强化健康管理理念和教职工的健康意识, 尽可能改善教职工的健康状况.     

微量元素硒的抗癌作用

对硒在恶性肿瘤的防治及其作用机理进行了总结和讨论,硒通过调节机体免疫功能、介入致癌物的代谢、抑制癌细胞生长及抗过氧化作用等发挥防癌和抗癌功能。另外,硒对白血病的治疗引起了人们的重视。     

Structural effects on the antitumour activity of a series of di(4-substituted phenyl)tin dichloride complexes with nitrogen-donor ligands

Investigation of the antitumour activity of a series of diorganotin dichloride complexes (4-ZC₆H₄)₂SnCl₂·L₂, where Z = OMe, Me, F, Cl, and CF₃ and L₂ = 2,2′-bipyridyl (bipy), 1,10-phenanthroline (phen) and 2-aminomethylpyridine (amp) is reported. A number of these complexex are shown to exhibit reproducible activity in vivo towards P388 lymphocytic leukaemia in mice. ¹H NMR data are reported for an extended series of (4-ZC₆H₄)₄Sn and the parent dichlorides (4-ZC₆H₄)₂SnCl₂ of the above-mentioned complexes. A correlation is reported between Hammett substituent constants and ¹H chemical shift data. Attempts are made to relate the antitumour activity of the complexes to various structural factors. The dependence of antitumour activity on the electronic effect of group Z and the nature of the ligand L₂ is demonstrated.     

Pharmaceutical Drug Metformin and MCL1 Inhibitor S63845 Exhibit Anticancer Activity in Myeloid Leukemia Cells via Redox Remodeling

Metabolic landscape and sensitivity to apoptosis induction play a crucial role in acute myeloid leukemia (AML) resistance. Therefore, we investigated the effect of metformin, a medication that also acts as an inhibitor of oxidative phosphorylation (OXPHOS), and MCL-1 inhibitor {"type":"entrez-nucleotide","attrs":{"text":"S63845","term_id":"400540","term_text":"S63845"}}S63845 in AML cell lines NB4, KG1 and chemoresistant KG1A cells. The impact of compounds was evaluated using fluorescence-based metabolic flux analysis, assessment of mitochondrial Δψ and cellular ROS, trypan blue exclusion, Annexin V-PI and XTT tests for cell death and cytotoxicity estimations, also RT-qPCR and Western blot for gene and protein expression. Treatment with metformin resulted in significant downregulation of OXPHOS; however, increase in glycolysis was observed in NB4 and KG1A cells. In contrast, treatment with {"type":"entrez-nucleotide","attrs":{"text":"S63845","term_id":"400540","term_text":"S63845"}}S63845 slightly increased the rate of OXPHOS in KG1 and KG1A cells, although it profoundly diminished the rate of glycolysis. Generally, combined treatment had stronger inhibitory effects on cellular metabolism and ATP levels. Furthermore, results revealed that treatment with metformin, {"type":"entrez-nucleotide","attrs":{"text":"S63845","term_id":"400540","term_text":"S63845"}}S63845 and their combinations induced apoptosis in AML cells. In addition, level of apoptotic cell death correlated with cellular ROS induction, as well as with downregulation of tumor suppressor protein MYC. In summary, we show that modulation of redox-stress could have a potential anticancer activity in AML cells.     

大螺距低剂量肺部CT技术在COPD患者中的应用价值

选择2014年1月~2018年12月本院呼吸内科住院部收治的拟实施肺部CT扫描的270例慢性阻塞性肺疾病(COPD)患者作为研究对象,根据不同螺距和辐射剂量,将患者分为大螺距低剂量组、小螺距低剂量组和大螺距常规剂量组,探讨大螺距低剂量肺部CT技术在COPD患者中的应用价值。结果显示,大螺距低剂量组肺实质CT均值标准差和伪影发生率比小螺距低剂量组明显降低,而SNR、CNR和图像质量评分较之明显增高(P<0.05)。大螺距低剂量组扫描时间明显短于另两组(P<0.05),CTDIvol、DLP和ED明显低于大螺距常规剂量组(P<0.05)。大螺距低剂量肺部CT技术在COPD患者中的应用价值满意,可明显提高图像质量和降低辐射剂量。