Polyphenolic profile and antioxidant effects of various parts of Artemisia annua L.

An annual Korean weed, Artemisia annua L., has been used as a folk medicine for the treatment of a number of diseases. Remarkably, among the 32 polyphenols characterized in various parts of plant tissue, including flowers, leafs, stems and roots, 10 compounds were detected for the first time using liquid chromatography–tandem mass spectrometry (LC/MS/MS). The quantification method was validated using structurally related external standards with determination coefficients (R²) ≥0.9995. The limits of detection and quantitation were 0.068–3.932 and 0.226–13.108 mg/L, respectively. The recoveries estimated at 50 and 100 mg/L ranged between 60.6–92.2 and 61.3–111%, respectively, with relative standard deviations <12%. The roots contained the largest concentration of identified components, while the flowers contained the least. The antioxidant capacity evaluated in terms of 1,1‐diphenyl‐2‐picrylhydrazyl and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical cation‐scavenging activities and reducing power was highest in the roots and lowest in the flowers. The findings are well correlated and suggest that the antioxidant capacities principally depend upon the polyphenol concentrations in each part of the plant. Copyright © 2015 John Wiley & Sons, Ltd.     

桂油与β-环糊精包合物的研究

采用共沉淀法制备了桂油与 β -环糊精的包合物 ,并用差热分析仪和气相色谱仪对包合物进行了分析 ,研究结果表明该方法包合桂油有利用率高、操作简便等优点 ,有利于拓宽桂油的应用面。     

钯－硝酸镁基体改进剂石墨炉原子吸收光谱法测定六味地黄丸中锡

本实验建立了钯－硝酸镁基体改进剂石墨炉原子吸收光谱法测定六味地黄丸中锡的方法。研究了基体改进剂的加入方式对测定的影响。方法的相对标准偏差为２．５％，回收率在９０％～１１０％之间，检出限为０．３ｎｇ／ｍＬ。本方法可用于中成药中锡的测定。     

Thermoluminescent (TL) trap characteristics in irradiated oregano herb

The aim of this article is to investigate in a very simple way the trap characteristics of the irradiated oregano herb. The preparation of the polymineral dust obtained from the herb is described and then, through a fading experiment, the characteristics of the trapping levels responsible of the thermoluminescent emission are discussed.     

电感耦合等离子体原子发射光谱/质谱法在中药微量元素及形态分析中的应用

本文对近年来电感耦合等离子体原子发射光谱法（ICP－AES）和电感耦合等离子体质谱（ICP－MS）在中药微量元素及形态分析中的应用进行了评述，讨论了应用ICP－AES／MS技术测定中药微量元素样品处理和各种分析方法，引用文献58篇。微波消解可作为中药微量元素分析理想的样品处理方法；ICP－AES／MS及各种联用技术在中药微量元素含量及形态分析中将发挥越来越重要的作用。     

Nitrogen-doped Carbon Dots: Application of Hg Ions Detection in Rannasangpei

Nitrogen-doped carbon dots(N-CDs) were hydrothermally synthesized by folic acid, which revealed excited wavelengtli-dependent blue emission and the specific recognition ofHg ions by fluorescence quenching. A wide range response and a fitted linear relationsliip for Hg ions detection were established to show that tliese blue-emission N-CDs achieved a satisfying Hg ions detection in Chinese traditional herb, Rannasaiigpei(RSNP). Tlie results from the N-CDs FL quenching gave the similar outcomes with those from ICP-OES measurement, which exhibited the possibility of the simple FL method of Hg ions detection by N-CDs in Chinese traditional herbs.     

Comparative analysis of main bio‐active components in the herb pair Danshen‐Honghua and its single herbs by ultra‐high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

A sensitive, reliable, and powerful ultra‐high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method was developed for simultaneous quantification of the 15 main bio‐active components including phenolic acids and flavonoids within 13 min for the first time. The proposed method was first reported and validated by good linearity (r ² > 0.9975), limit of detection (1.12–7.01 ng/mL), limit of quantification (3.73–23.37 ng/mL), intra‐ and inter‐day precisions (RSD ≤ 1.92%, RSD ≤ 2.45%), stability (RSD ≤ 5.63%), repeatability (RSD ≤ 4.34%), recovery (96.84–102.12%), and matrix effects (0.92–1.02). The established analytical methodology was successfully applied to comparative analysis of main bio‐active components in the herb pair Danshen‐Honghua and its single herbs. Compared to the single herb, the content of most flavonoid glycosides was remarkably increased in their herb pair, and main phenolic acids were decreased, conversely. The content changes of the main components in the herb pair supported the synergistic effects on promoting blood circulation and removing blood stasis. The results provide a scientific basis and reference for the quality control of Danshen‐Honghua herb pair and the drug interactions based on variation of bio‐active components in herb pairs.     

The Changes in Bioactive Compounds and Antioxidant Activity of Chia (Salvia hispanica L.) Herb under Storage and Different Drying Conditions: A Comparison with Other Species of Sage

Studies on herb chia (Salvia hispanica L.) are very limited. Therefore, the aim of this study was to assess how different drying methods and periods of storage affect the bioactive properties of the herb Salvia hispanica and to compare it with other species of sage (Salvia officinalis L. and Salvia sclarea L.). In fresh herbs, directly after drying (freeze-drying, natural drying, and drying at 30, 40, and 50 °C), and after storage (3, 6, and 12 months), the following analyses were performed: content of total carotenoids and total polyphenols, polyphenol profile (including 25 compounds), and antioxidant activity. Additionally, the basic chemical compositions of the herbs were analyzed. To the best of our knowledge, the content of total carotenoids and the quantitative polyphenol profile in Salvia hispanica and Salvia sclarea were evaluated for the first time. The obtained results showed that the barely investigated herb Salvia hispanica is rich in polyphenolic compounds and shows high antioxidant activity. In all the tested species, rosmarinic acid was the most abundant polyphenolic compound. The use of different drying methods allowed us to determine that freeze-drying was the most effective for preserving polyphenols and carotenoids. Long-term storage up to 12 months resulted in a gradual reduction in antioxidant activity and in the content of polyphenols and carotenoids.     

中药材全息指纹谱图库的建立

 设计并建立了中药材全息指纹谱图库软件 ,用于存放各种指纹谱及其相关信息。该数据库将为中药分析及其质量控制提供帮助与参考。